Zobrazeno 1 - 3
of 3
pro vyhledávání: '"Silvia Y. L. Mak"'
Autor:
Dawn G. L. Thean, Hoi Yee Chu, John H. C. Fong, Becky K. C. Chan, Peng Zhou, Cynthia C. S. Kwok, Yee Man Chan, Silvia Y. L. Mak, Gigi C. G. Choi, Joshua W. K. Ho, Zongli Zheng, Alan S. L. Wong
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-14 (2022)
Screening combinatorial mutants is too massive for wet-lab experiment alone. Here the authors present a machine learning-coupled combinatorial mutagenesis approach to vastly reduce experimental burden for engineering Cas9 genome editing enzymes.
Externí odkaz:
https://doaj.org/article/f615b869b7234e9f9215dee850d25dff
Autor:
Chaya T L Yuen, Dawn G L Thean, Becky K C Chan, Peng Zhou, Cynthia C S Kwok, Hoi Yee Chu, Maggie S H Cheung, Bei Wang, Yee Man Chan, Silvia Y L Mak, Anskar Y Leung, Gigi C G Choi, Zongli Zheng, Alan S L Wong
Publikováno v:
Nucleic acids research. 50(3)
The Cas9 nuclease from Staphylococcus aureus (SaCas9) holds great potential for use in gene therapy, and variants with increased fidelity have been engineered. However, we find that existing variants have not reached the greatest accuracy to discrimi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a56ddb3fcbd02e8269626278c0488f7a
https://pubmed.ncbi.nlm.nih.gov/35051997
https://pubmed.ncbi.nlm.nih.gov/35051997
Autor:
Dawn G. L. Thean, Hoi Yee Chu, John H. C. Fong, Becky K. C. Chan, Peng Zhou, Cynthia C. S. Kwok, Yee Man Chan, Silvia Y. L. Mak, Gigi C. G. Choi, Joshua W. K. Ho, Zongli Zheng, Alan S. L. Wong
Publikováno v:
Nature communications. 13(1)
The genome-editing Cas9 protein uses multiple amino-acid residues to bind the target DNA. Considering only the residues in proximity to the target DNA as potential sites to optimise Cas9’s activity, the number of combinatorial variants to screen th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e649bfee27356473e0372a05cec61a95
https://pubmed.ncbi.nlm.nih.gov/35468907
https://pubmed.ncbi.nlm.nih.gov/35468907