Výsledky vyhledávání - "Silvia Merli"

Akademický článek

From Placenta to Polycystic Ovarian Syndrome: The Role of Adipokines

Autor: Chiara Sartori, Pietro Lazzeroni, Silvia Merli, Viviana Dora Patianna, Francesca Viaroli, Francesca Cirillo, Sergio Amarri, Maria Elisabeth Street

Publikováno v: Mediators of Inflammation, Vol 2016 (2016)

Adipokines are cytokines produced mainly by adipose tissue, besides many other tissues such as placenta, ovaries, peripheral-blood mononuclear cells, liver, muscle, kidney, heart, and bone marrow. Adipokines play a significant role in the metabolic s

Externí odkaz: https://doaj.org/article/3e8038d5c9464ea48729595331774278

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Production of Soluble Tumor Necrosis Factor Receptor Type I in Escherichia coli: Optimization of the Refolding Yields by a Microtiter Dilution Assay

Autor: Giovanni Cassani, Silvia Merli, Angelo Corti

Publikováno v: Analytical Biochemistry. 230:85-91

In this study optimization of the soluble tumor necrosis factor receptor type I (sTNF-RI) refolding by the use of a micro-renaturation assay in 96-well microplates is described. Microplate wells were filled with buffers varying in pH and urea and sub

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c291dbb243de81383148e6fb9a4c0369
https://doi.org/10.1006/abio.1995.1441

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High Yield Expression and Purification of Human Endothelin-1

Autor: Silvia Merli, S. Germani, Giovanni Cassani, Giorgio Fassina, Gennaro Ciliberto

Publikováno v: Protein Expression and Purification. 5:559-568

A DNA construct encoding human big endothelin (Big ET) preceded by the factor Xa protease recognition site (Ile-Glu-Gly-Arg), fused in frame to the maltose binding protein sequence, has been introduced in DH5-alpha cells. The fusion product (MBP-Big

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad986445633e603ce19937f1b346cf16
https://doi.org/10.1006/prep.1994.1077

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An expression system for the single-step production of recombinant human amidated calcitonin

Autor: Giovanni Cassani, Matteo Villain, Maria Tortora, Silvia Merli, Giorgio Fassina, Antonio Verdoliva, Sandro De Falco, Patrizio Silvi

Publikováno v: Protein expression and purification
7 (1996): 347–354. doi:10.1006/prep.1996.0052
info:cnr-pdr/source/autori:Merli S, De Falco S, Verdoliva A, Tortora M, Villain M, Silvi P, Cassani G, Fassina G/titolo:An expression system for the single-step production of recombinant human amidated calcitonin/doi:10.1006%2Fprep.1996.0052/rivista:Protein expression and purification (Print)/anno:1996/pagina_da:347/pagina_a:354/intervallo_pagine:347–354/volume:7

Amidating mouse pituitary cells (AtT-20) have been engineered to secrete human calcitonin (hCT) in the fully active amidated form, without the need of additional enzymatic or chemical modifications. The 141-residue human calcitonin precursor has firs

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::326d359771b9482a1403c061bb3506e1
https://pubmed.ncbi.nlm.nih.gov/8776751

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Identification of two forms (31-33 and 48 kD) of the urinary soluble p55 tumor necrosis factor receptor that are differentially N- and O-glycosylated

Autor: Angelo Corti, Giovanni Cassani, Silvia Merli, Maria Marino, Luca Bagnasco, Fabrizio D'ambrosio

The structure and the activity of urinary soluble TNF receptor type 1 (sTNF-R1), isolated from the urine of normal individuals, has been characterized and compared with that of recombinant sTNF-R1 expressed in CHO cells and with that of a nonglycosyl

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a420267fe09fb40521363454443d0dbf
http://hdl.handle.net/11567/625555

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Tumor-necrosis-factor (TNF)-alpha quantification by ELISA and bioassay - effects of TNF-alpha-soluble TNF-receptor (p55) complex dissociation during assay incubations

Autor: Giovanni Cassani, Silvia Merli, Claudio Poiesi, Angelo Corti

It has been previously reported that different quantitative results can be obtained when TNF alpha is measured in biological fluids by bioassay and immunoassay. This is thought to be related to the presence of antigenic forms of TNF alpha that cannot

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9bfdefe8260b10176b63d42ec140fa35

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Identification of hydropathically complementary putative contact sequences within epidermal growth factor (EGF) and the EGF receptor

Autor: Siegfried Schwarz, G. Enrico Rovati, Silvia Merli

Publikováno v: Life sciences. 51(1)

Hydropathic complementaries (HC) has been proposed as a novel molecular recognition code for how two proteins can recognize one other and thus form a reversible complex. If a protein contains a segment of a few amino acid residues that is surface-exp

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3feb44979464b17b122d3e2b0056183c
https://pubmed.ncbi.nlm.nih.gov/1614273

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A Single Amino Acid Interchange Yields Reciprocal CTL Specificities for HIV-1 gp160

Autor: Silvia Merli, Richard A. Houghten, Jay A. Berzofsky, Scott D. Putney, Hidemi Takahashi, Bernard Moss, Ronald N. Germain

Publikováno v: Science. 246:118-121

For the IIIB isolate of human immunodeficiency virus type-1 (HIV-1), the immunodominant determinant of the envelope protein gp160 for cytotoxic T lymphocytes (CTLs) of H-2d mice is in a region of high sequence variability among HIV-1 isolates. The ge

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::277fdabeab94b102dac0613b516f8cb0
https://doi.org/10.1126/science.2789433

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Akademický článek

Poster Presentations: pp. 151-248.

Publikováno v: Hormone Research in Paediatrics; Sep2012 Supplement, Vol. 78, p151-248, 98p, 3 Color Photographs, 1 Black and White Photograph, 48 Charts, 6 Graphs

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