Evaluation of two in vitro assays for tumorigenicity assessment of CRISPR-Cas9 genome-edited cells

Autor: Myriam Lemmens, Benoit Fischer, Michael Zogg, Lindsey Rodrigues, Grainne Kerr, Alberto del Rio-Espinola, Fanny Schaeffer, Danilo Maddalo, Valerie Dubost, Alessandro Piaia, Arne Mueller, Ulla Plappert-Helbig, Ulrike Naumann, Jasmin Haegele, Alex Odermatt, Hans-Jörg Martus, Silvana Libertini

Publikováno v: Molecular Therapy: Methods & Clinical Development, Vol 23, Iss , Pp 241-253 (2021)

Off-target editing is one of the main safety concerns for the use of CRISPR-Cas9 genome editing in gene therapy. These unwanted modifications could lead to malignant transformation, which renders tumorigenicity assessment of gene therapy products ind

Externí odkaz: https://doaj.org/article/290bd942646a411a99a58b2d82349198

Identification of marker genes to monitor residual iPSCs in iPSC-derived products

Autor: Myriam Lemmens, Juliane Perner, Leon Potgeter, Michael Zogg, Sineha Thiruchelvam, Matthias Müller, Thierry Doll, Annick Werner, Yoann Gilbart, Philippe Couttet, Hans-Jörg Martus, Silvana Libertini

Publikováno v: Cytotherapy. 25:59-67

Engineered tissues and cell therapies based on human induced pluripotent stem cells (iPSCs) represent a promising approach for novel medicines. However, iPSC-derived cells and tissues may contain residual undifferentiated iPSCs that could lead to ter

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::77e611493a35ce4d60ff2f14fa84fd93
https://doi.org/10.1016/j.jcyt.2022.09.010

Gamma-H2AX immunofluorescence for the detection of tissue-specific genotoxicity in vivo

Autor: Diethilde Theil, Wilfried Frieauff, Silvana Libertini, Hans-Jörg Martus, Ulla Plappert-Helbig

Publikováno v: Environmental and Molecular Mutagenesis. 60:4-16

The phosphorylation of histone H2AX in Serine 139 (gamma-H2AX) marks regions of DNA double strand breaks and contributes to the recruitment of DNA repair factors to the site of DNA damage. Gamma-H2AX is used widely as DNA damage marker in vitro, but

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::dab91305a631f8c24b1a638350da762f
https://doi.org/10.1002/em.22238

Generation of renal Epo-producing cell lines by conditional gene tagging reveals rapid HIF-2 driven Epo kinetics, cell autonomous feedback regulation, and a telocyte phenotype

Autor: Edith Hummler, Sophie L. Dahl, Silvana Libertini, Andreas M. Bapst, Carsten C. Scholz, Ilaria M.C. Orlando, Svende Pfundstein, Faik Imeri, Roland H. Wenger, Patrick Spielmann, Karen A. Nolan, Lisa Crowther, Willy Kuo, Sara Santambrogio, David Hoogewijs, Anna Keodara, Irene Abreu-Rodríguez, Vartan Kurtcuoglu, Weihong Qi

Publikováno v: Kidney international. 95(2)

Erythropoietin (Epo) is essential for erythropoiesis and is mainly produced by the fetal liver and the adult kidney following hypoxic stimulation. Epo regulation is commonly studied in hepatoma cell lines, but differences in Epo regulation between ki

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7c174cf71f52ea61c428ac263bae7e1b
https://pubmed.ncbi.nlm.nih.gov/30502050

Inactivation of TGFβ receptors in stem cells drives cutaneous squamous cell carcinoma

Autor: Owen J. Sansom, Catherine A. Harwood, Gareth J. Inman, Jun Wang, Philip J. Coates, Richard Marais, Nick Barker, Hans Clevers, Jonas Larsson, Lindsay C. Spender, Angela McHugh, Ai Nagano, Catrin Pritchard, Charlotte M. Proby, Celine Pourreyron, Karin J. Purdie, Dimitris Athineos, Aidan M. Rose, Simone Weidlich, Rachel A. Ridgway, Patrizia Cammareri, Claude Chelala, David F. Vincent, Stefan Karlsson, Irene M. Leigh, Gopal P. Sapkota, Andrew P. South, Silvana Libertini, Jasbani H.S. Dayal

Publikováno v: Cammareri, P, Rose, A M, Vincent, D F, Wang, J, Nagano, A, Libertini, S, Ridgway, R A, Athineos, D, Coates, P J, McHugh, A, Pourreyron, C, Dayal, J H S, Larsson, J, Weidlich, S, Spender, L C, Sapkota, G P, Purdie, K J, Proby, C M, Harwood, C A, Leigh, I M, Clevers, H, Barker, N, Karlsson, S, Pritchard, C, Marais, R, Chelala, C, South, A P, Sansom, O J & Inman, G J 2016, ' Inactivation of TGFβ receptors in stem cells drives cutaneous squamous cell carcinoma ', Nature Communications, vol. 7, 12493 . https://doi.org/10.1038/ncomms12493
Nature Communications, 7. Nature Publishing Group
Nature Communications [E], 7. Nature Publishing Group
Nature Communications, Vol 7, Iss 1, Pp 1-14 (2016)
Nature Communications

Melanoma patients treated with oncogenic BRAF inhibitors can develop cutaneous squamous cell carcinoma (cSCC) within weeks of treatment, driven by paradoxical RAS/RAF/MAPK pathway activation. Here we identify frequent TGFBR1 and TGFBR2 mutations in h

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c3436b88b2c09034682df9ba1b22d0f5
https://www.research.manchester.ac.uk/portal/en/publications/inactivation-of-tgf-receptors-in-stem-cells-drives-cutaneous-squamous-cell-carcinoma(dbbe84d0-3ec2-4c50-a957-399731e49e6f).html