On the humanization of VHHs: Prospective case studies, experimental and computational characterization of structural determinants for functionality.

Autor: Fernández-Quintero ML; Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, USA., Guarnera E; Antibody Discovery and Protein Engineering, Merck Healthcare KGaA, Darmstadt, Germany., Musil D; Structural Biology and Biophysics, Merck Healthcare KGaA, Darmstadt, Germany., Pekar L; Antibody Discovery and Protein Engineering, Merck Healthcare KGaA, Darmstadt, Germany., Sellmann C; Antibody Discovery and Protein Engineering, Merck Healthcare KGaA, Darmstadt, Germany., Freire F; iBET, Instituto de Biologia Experimental e Tecnológica, Oeiras, Portugal., Sousa RL; iBET, Instituto de Biologia Experimental e Tecnológica, Oeiras, Portugal., Santos SP; iBET, Instituto de Biologia Experimental e Tecnológica, Oeiras, Portugal., Freitas MC; iBET, Instituto de Biologia Experimental e Tecnológica, Oeiras, Portugal., Bandeiras TM; iBET, Instituto de Biologia Experimental e Tecnológica, Oeiras, Portugal., Silva MMS; iBET, Instituto de Biologia Experimental e Tecnológica, Oeiras, Portugal., Loeffler JR; Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, USA., Ward AB; Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, USA., Harwardt J; Antibody Discovery and Protein Engineering, Merck Healthcare KGaA, Darmstadt, Germany., Zielonka S; Antibody Discovery and Protein Engineering, Merck Healthcare KGaA, Darmstadt, Germany.; Institute for Organic Chemistry and Biochemistry, Technical University of Darmstadt, Darmstadt, Germany., Evers A; Antibody Discovery and Protein Engineering, Merck Healthcare KGaA, Darmstadt, Germany.

Publikováno v: Protein science : a publication of the Protein Society [Protein Sci] 2024 Nov; Vol. 33 (11), pp. e5176.