Zobrazeno 1 - 10
of 43
pro vyhledávání: '"Siewert Hugelier"'
Autor:
Changsong Yang, Patricia Colosi, Siewert Hugelier, Daniel Zabezhinsky, Melike Lakadamyali, Tatyana Svitkina
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-20 (2022)
The role of actin filaments in clathrin-mediated endocytosis (CME) is unclear. Here, Yang et al. show that branched actin filaments promote CME by pushing on clathrin coat edges in an epsin-dependent manner, dividing large flat clathrin plaques into
Externí odkaz:
https://doaj.org/article/78d839177d0345c99e08b41d83acdbfa
Autor:
Siewert Hugelier, Wim Vandenberg, Tomáš Lukeš, Kristin S. Grußmayer, Paul H. C. Eilers, Peter Dedecker, Cyril Ruckebusch
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
Abstract Sub-diffraction or super-resolution fluorescence imaging allows the visualization of the cellular morphology and interactions at the nanoscale. Statistical analysis methods such as super-resolution optical fluctuation imaging (SOFI) obtain a
Externí odkaz:
https://doaj.org/article/f9a2e049d318479aae5d45359e047cf9
Autor:
Thijs Roebroek, Wim Vandenberg, François Sipieter, Siewert Hugelier, Christophe Stove, Jin Zhang, Peter Dedecker
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
Performing multiple FRET measurements at once can be challenging. Here the authors report a method to discriminate between overlapping FRET pairs, even if the fluorophores display almost identical absorption and emission spectra, based on the photoch
Externí odkaz:
https://doaj.org/article/9e086db852c1424faa90ec134becce7a
Autor:
Hana Valenta, Siewert Hugelier, Sam Duwé, Giulia Lo Gerfo, Marcel Müller, Peter Dedecker, Wim Vandenberg
Publikováno v:
Biophysical Reports, Vol 1, Iss 2, Pp 100026- (2021)
Multicolor fluorescence imaging is an excellent method for the simultaneous visualization of multiple structures, although it is limited by the available spectral window. More labels can be measured by distinguishing these on properties, such as thei
Externí odkaz:
https://doaj.org/article/dc37e708950f4e82a2bfaa29e64e5bf1
Autor:
Siewert Hugelier, Patrizia Firmani, Olivier Devos, Myriam Moreau, Christel Pierlot, Federico Marini, Cyril Ruckebusch
Publikováno v:
Journal of Spectral Imaging, Vol 5, p a7 (2016)
In order to investigate hyperspectral images, many techniques such as multivariate image analysis (MIA) or multivariate curve resolution–alternating least squares (MCR–ALS) can be applied. When focusing on the use of MCR–ALS, constraints are of
Externí odkaz:
https://doaj.org/article/39aa0ea4afe74c759716dea18858498b
Publikováno v:
Annual Review of Biophysics. 52:139-160
Super-resolution fluorescence microscopy allows the investigation of cellular structures at nanoscale resolution using light. Current developments in super-resolution microscopy have focused on reliable quantification of the underlying biological dat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7dd6d73b3220daeb2caccd48f213cddd
https://doi.org/10.1146/annurev-biophys-111622-091212
https://doi.org/10.1146/annurev-biophys-111622-091212
Autor:
Siewert Hugelier, Hannah Kim, Melina Theoni Gyparaki, Charles Bond, Qing Tang, Adriana Naomi Santiago-Ruiz, Sílvia Porta, Melike Lakadamyali
Publikováno v:
bioRxiv
We introduce a new automated machine learning analysis pipeline to precisely classify cellular structures captured through single molecule localization microscopy, which we call ECLiPSE (Enhanced Classification of Localized Pointclouds by Shape Extra
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7e3005b71958a2f92e5c827c7685182b
https://europepmc.org/articles/PMC10197633/
https://europepmc.org/articles/PMC10197633/
Autor:
Robin Van den Eynde, Fabian Hertel, Bartosz Krajnik, Siewert Hugelier, Alexander Auer, Thomas Schlichthaerle, Ralf Jungmann, Marcel Leutenegger, Wim Vandenberg, Peter Dedecker
We present a novel way to enhance the information content of fluorescence imaging by encoding information in the microscope point-spread function (PSF). In contrast to methods that modify the shape of the PSF itself, our approach encodes the addition
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::72fcb27c28ebccd983cb5e45dfc964f0
https://doi.org/10.1101/2022.10.04.510770
https://doi.org/10.1101/2022.10.04.510770
Autor:
Qing Tang, Sebastian Sensale, Charles Bond, Andy Qiao, Siewert Hugelier, Arian Arab, Gaurav Arya, Melike Lakadamyali
Microtubules in cells consist of functionally diverse subpopulations carrying distinct post-translational modifications (PTMs). Akin to the histone code, the tubulin code regulates a myriad of microtubule functions ranging from intracellular transpor
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9962322a26b20d219b8b0e2a8004b551
https://doi.org/10.1101/2022.09.29.510213
https://doi.org/10.1101/2022.09.29.510213
Publikováno v:
Biophysical Journal. 122:129a-130a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::497e80195f2376d04b544ad09030f87e
https://doi.org/10.1016/j.bpj.2022.11.866
https://doi.org/10.1016/j.bpj.2022.11.866