Liquid-nitrogen-free CTAB DNA extraction method from silica-dried specimens for next-generation sequencing and assembly.

Autor: Quiñones KJO; Institute of Crop Science, College of Agriculture and Food Science, University of the Philippines Los Baños, College, Laguna, Philippines.; Department of Science and Technology, Science Education Institute, Taguig, Manila, Philippines., Gentallan RP Jr; Institute of Crop Science, College of Agriculture and Food Science, University of the Philippines Los Baños, College, Laguna, Philippines., Timog EBS; Department of Forest Biological Sciences, College of Forestry and Natural Resources, University of the Philippines Los Baños, College, Laguna, Philippines., Cruz JRAV; Department of Science and Technology, Science Education Institute, Taguig, Manila, Philippines.; Philippine Rice Research Institute, Maligaya, Science City of Muñoz, Nueva Ecija, Philippines., Macabecha CGA; Department of Science and Technology, Science Education Institute, Taguig, Manila, Philippines.; National Institute of Molecular Biology and Biotechnology (BIOTECH-UPLB), College of Agriculture and Food Science, University of the Philippines Los Baños, College, Laguna, Philippines., Papa IA; National Institute of Molecular Biology and Biotechnology (BIOTECH-UPLB), College of Agriculture and Food Science, University of the Philippines Los Baños, College, Laguna, Philippines., Coronado NB; Institute of Biological Sciences, College of Arts and Sciences, University of the Philippines Los Baños, College, Laguna, Philippines., Bartolome MCB; Department of Science and Technology, Science Education Institute, Taguig, Manila, Philippines., Ceribo DB; Department of Science and Technology, Science Education Institute, Taguig, Manila, Philippines., Madayag RE; Agricultural Systems Institute, College of Agriculture and Food Science, University of the Philippines Los Baños, College, Laguna, Philippines., Magtoltol JB; Agricultural Systems Institute, College of Agriculture and Food Science, University of the Philippines Los Baños, College, Laguna, Philippines., Sienes RKA; Department of Science and Technology, Science Education Institute, Taguig, Manila, Philippines.; Research Division, Mindanao State University at Naawan, Naawan, Misamis Oriental, Philippines., Alvaran BBS; Institute of Crop Science, College of Agriculture and Food Science, University of the Philippines Los Baños, College, Laguna, Philippines.; International Rice Research Institute, Los Baños, Laguna, Philippines., Borromeo TH; Institute of Crop Science, College of Agriculture and Food Science, University of the Philippines Los Baños, College, Laguna, Philippines.

Publikováno v: MethodsX [MethodsX] 2024 May 22; Vol. 12, pp. 102758. Date of Electronic Publication: 2024 May 22 (Print Publication: 2024).