Zobrazeno 1 - 10
of 25
pro vyhledávání: '"Siegfried Detke"'
Autor:
Siegfried Detke, R. Elsabrouty
Publikováno v:
Experimental Parasitology. 118:408-419
Leishmania cannot synthesize purines de novo and rely on their host to furnish these compounds. To accomplish this, they possess multiple purine nucleoside and nucleobase transporters. Subcellular fractionation, immunohistochemical localization with
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::063f08b38177addb72852686c2162036
https://doi.org/10.1016/j.exppara.2007.10.010
https://doi.org/10.1016/j.exppara.2007.10.010
Autor:
Siegfried Detke
Publikováno v:
Experimental Cell Research. 313:1963-1978
TOR is an atypical multidrug resistance protein present in the human protozoan parasite, Leishmania. Resistance to the toxic adenosine analog tubercidin was brought about by redirecting the adenosine permease from the plasma membrane to the multivesi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::120dab24c23d331ceea36af3f42cd82d
https://doi.org/10.1016/j.yexcr.2007.02.027
https://doi.org/10.1016/j.yexcr.2007.02.027
Autor:
Siegfried Detke
Publikováno v:
Experimental Parasitology. 115:221-225
A number of peptide tags are available to facilitate the characterization of recombinant proteins. We have tested the bacterial oxaloacetate decarboxylase biotinylation domain for its efficacy in tagging recombinant proteins in vivo in Leishmania. To
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::12cad1490a10fb8d2230f060e28de5ee
https://doi.org/10.1016/j.exppara.2006.08.009
https://doi.org/10.1016/j.exppara.2006.08.009
Autor:
Siegfried Detke
Publikováno v:
Yeast. 14:1257-1265
The nucleoside permease gene (i.e. NUP) from Candida albicans was cloned by complementation of Saccharomyces cerevisiae deficient in nucleoside transport capability. The permease transported adenosine and guanosine and was sensitive to the mammalian
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ef435a631721715a6e8ae17791bd5b82
https://doi.org/10.1002/(sici)1097-0061(1998100)14:14<1257::aid-yea326>3.0.co
https://doi.org/10.1002/(sici)1097-0061(1998100)14:14<1257::aid-yea326>3.0.co
Autor:
Siegfried Detke, Bryan R. Kerby
Publikováno v:
Molecular and Biochemical Parasitology. 60:171-185
Nucleoside analogs are potential anti- Leishmania agents. To better understand how these compounds might lose their effectiveness, Leishmania were independently selected for resistance to inosine dialdehyde or tubercidin. Each of the resistant cells
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1198a1ff127550b9f3241daed725ca73
https://doi.org/10.1016/0166-6851(93)90129-l
https://doi.org/10.1016/0166-6851(93)90129-l
Autor:
Diana, Ortiz, Raquel, Valdés, Marco A, Sanchez, Johanna, Hayenga, Carolyn, Elya, Siegfried, Detke, Scott M, Landfear
Publikováno v:
Molecular microbiology. 78(1)
Leishmania and other parasitic protozoa are unable to synthesize purines de novo and are reliant upon purine nucleoside and nucleobase transporters to import preformed purines from their hosts. To study the roles of the four purine permeases NT1-NT4
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::d915a8c07dfd4eaa5f44b074368babf9
https://pubmed.ncbi.nlm.nih.gov/20735779
https://pubmed.ncbi.nlm.nih.gov/20735779
Identification of a mitochondrial ATP synthase-adenine nucleotide translocator complex in Leishmania
Autor:
Siegfried Detke, Rania Elsabrouty
Publikováno v:
Acta tropica. 105(1)
The ATP synthasome is a macromolecular complex consisting of ATP synthase, adenine nucleotide translocator and phosphate carrier. To determine if this complex is evolutionary old or young, we searched for its presence in Leishmania, a mitochondria co
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1736ecb21c04a1b1d4ae657d3913b599
https://pubmed.ncbi.nlm.nih.gov/17920025
https://pubmed.ncbi.nlm.nih.gov/17920025
Autor:
Siegfried Detke
Publikováno v:
Molecular and biochemical parasitology. 90(2)
The TOR gene (TOxic nucleoside Resistance gene) was mapped to a 2.3 kb fragment on the amplified DNA from tubercidin resistant Leishmania (TUB). This DNA fragment conferred upon wild type cells resistance to tubercidin, inosine dialdehyde, formycin A
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d5fa9fba3e687b2b897a4959c78b4ef9
https://pubmed.ncbi.nlm.nih.gov/9476797
https://pubmed.ncbi.nlm.nih.gov/9476797
Publikováno v:
Molecular and biochemical parasitology. 44(2)
Twelve independent variants were selected from five species of Leishmania for resistance to tunicamycin by exposure of cultured promastigotes to increasing concentrations of this antibiotic, an inhibitor of the microsomal N-acetylglucosamine-1-phosph
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eb8690468e82fd3da6042c0230adc6b1
https://pubmed.ncbi.nlm.nih.gov/1646959
https://pubmed.ncbi.nlm.nih.gov/1646959
Autor:
Marvin R. Paule, Siegfried Detke
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis. 520:131-138
Multiple forms of class III DNA-dependent RNA polymerase have been found in a number of higher eukaryotic cell types (Chambon, P. (1975) Annu. Rev. Biochem. 44, 613–638). Similar multiple forms are reported here from a lower eukaryote, the soil amo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7ff1ec1623c525e25aeb5e84219a52e0
https://doi.org/10.1016/0005-2787(78)90014-x
https://doi.org/10.1016/0005-2787(78)90014-x