FALCON systematically interrogates free fatty acid biology and identifies a novel mediator of lipotoxicity.

Autor: Wieder N; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, USA; Harvard Medical School, Boston, MA 02115, USA; Department of Neurology with Experimental Neurology and Berlin Institute of Health, Charité, 10117 Berlin, Germany., Fried JC; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, USA; Harvard Medical School, Boston, MA 02115, USA., Kim C; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, USA; Harvard Medical School, Boston, MA 02115, USA., Sidhom EH; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, USA; Harvard Medical School, Boston, MA 02115, USA., Brown MR; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA., Marshall JL; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA., Arevalo C; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA., Dvela-Levitt M; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, USA; Harvard Medical School, Boston, MA 02115, USA; The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel., Kost-Alimova M; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA., Sieber J; Department of Endocrinology, Metabolism and Cardiovascular Systems, University of Fribourg, Fribourg, Switzerland., Gabriel KR; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA., Pacheco J; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA., Clish C; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA., Abbasi HS; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA., Singh S; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA., Rutter JC; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Harvard Medical School, Boston, MA 02115, USA., Therrien M; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA., Yoon H; Department of Cell Biology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USA; Ludwig Center for Cancer Research at Harvard, Boston, MA 02115, USA., Lai ZW; Harvard Chan Advanced Multiomics Platform, Harvard T.H. Chan School of Public Health, Boston, MA 02115, USA., Baublis A; Harvard Chan Advanced Multiomics Platform, Harvard T.H. Chan School of Public Health, Boston, MA 02115, USA., Subramanian R; Laboratory for Surgical and Metabolic Research, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA., Devkota R; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Chemical Biology and Therapeutics Science, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA., Small J; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Harvard Medical School, Boston, MA 02115, USA; Chemical Biology and Therapeutics Science, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA., Sreekanth V; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Divisions of Renal Medicine and Engineering, Brigham and Women's Hospital, Boston, MA 02115, USA., Han M; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA., Lim D; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA., Carpenter AE; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA., Flannick J; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Harvard Medical School, Boston, MA 02115, USA; Division of Genetics and Genomics, Boston Children's Hospital, Boston, MA 02115, USA., Finucane H; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Analytic and Translational Genetics Unit, Mass General Hospital, Boston, MA 02114, USA., Haigis MC; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Department of Cell Biology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USA; Ludwig Center for Cancer Research at Harvard, Boston, MA 02115, USA., Claussnitzer M; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Harvard Medical School, Boston, MA 02115, USA; Metabolism Program, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA., Sheu E; Laboratory for Surgical and Metabolic Research, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA., Stevens B; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Harvard Medical School, Boston, MA 02115, USA; Boston Children's Hospital, F.M. Kirby Neurobiology Center, Boston, MA 02115, USA; Howard Hughes Medical Institute, Boston, MA 02115, USA., Wagner BK; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Chemical Biology and Therapeutics Science, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA., Choudhary A; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Harvard Medical School, Boston, MA 02115, USA; Chemical Biology and Therapeutics Science, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Divisions of Renal Medicine and Engineering, Brigham and Women's Hospital, Boston, MA 02115, USA., Shaw JL; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA., Pablo JL; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA., Greka A; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, USA; Harvard Medical School, Boston, MA 02115, USA. Electronic address: agreka@bwh.harvard.edu.

Publikováno v: Cell metabolism [Cell Metab] 2023 May 02; Vol. 35 (5), pp. 887-905.e11. Date of Electronic Publication: 2023 Apr 18.

Targeting a Braf/Mapk pathway rescues podocyte lipid peroxidation in CoQ-deficiency kidney disease.

Autor: Sidhom EH; Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, USA.; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA., Kim C; Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, USA.; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA., Kost-Alimova M; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA., Ting MT; Department of Neurology, Columbia University Medical Center, New York, New York, USA., Keller K; Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, USA., Avila-Pacheco J; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA., Watts AJ; Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, USA.; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA., Vernon KA; Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, USA.; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA., Marshall JL; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA., Reyes-Bricio E; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA., Racette M; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA., Wieder N; Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, USA.; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA., Kleiner G; Department of Neurology, Columbia University Medical Center, New York, New York, USA., Grinkevich EJ; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA., Chen F; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA., Weins A; Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, USA., Clish CB; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA., Shaw JL; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA., Quinzii CM; Department of Neurology, Columbia University Medical Center, New York, New York, USA., Greka A; Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, USA.; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.

Publikováno v: The Journal of clinical investigation [J Clin Invest] 2021 Mar 01; Vol. 131 (5).

A High Content Screen for Mucin-1-Reducing Compounds Identifies Fostamatinib as a Candidate for Rapid Repurposing for Acute Lung Injury during the COVID-19 pandemic.

Autor: Alimova M; The Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA., Sidhom EH; The Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.; Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, USA., Satyam A; Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts, USA., Dvela-Levitt M; The Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.; Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, USA., Melanson M; The Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA., Chamberlain BT; The Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA., Alper SL; The Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.; Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts, USA., Santos J; The Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA., Gutierrez J; The Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA., Subramanian A; The Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA., Grinkevich E; The Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA., Bricio ER; The Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA., Kim C; The Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.; Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, USA., Clark A; The Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA., Watts A; The Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.; Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, USA., Thompson R; The Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA., Marshall J; The Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA., Pablo JL; The Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA., Coraor J; The Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.; Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, USA., Roignot J; The Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA., Vernon KA; The Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.; Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, USA., Keller K; The Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.; Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, USA., Campbell A; The Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.; Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, USA., Emani M; The Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA., Racette M; The Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA., Bazua-Valenti S; The Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.; Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, USA., Padovano V; The Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA., Weins A; Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, USA., McAdoo SP; Department of Immunology and Inflammation, Imperial College, Hammersmith Hospital, London, UK., Tam FWK; Department of Immunology and Inflammation, Imperial College, Hammersmith Hospital, London, UK., Ronco L; The Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA., Wagner F; The Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA., Tsokos GC; Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts, USA., Shaw JL; The Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA., Greka A; The Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.; Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, USA.

Publikováno v: BioRxiv : the preprint server for biology [bioRxiv] 2020 Jun 30. Date of Electronic Publication: 2020 Jun 30.

Small Molecule Targets TMED9 and Promotes Lysosomal Degradation to Reverse Proteinopathy.

Autor: Dvela-Levitt M; Broad Institute of MIT and Harvard, Cambridge, MA, USA; Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA., Kost-Alimova M; Broad Institute of MIT and Harvard, Cambridge, MA, USA., Emani M; Broad Institute of MIT and Harvard, Cambridge, MA, USA; Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA., Kohnert E; Broad Institute of MIT and Harvard, Cambridge, MA, USA; Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA., Thompson R; Broad Institute of MIT and Harvard, Cambridge, MA, USA; Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA., Sidhom EH; Broad Institute of MIT and Harvard, Cambridge, MA, USA; Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA., Rivadeneira A; Broad Institute of MIT and Harvard, Cambridge, MA, USA; Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA., Sahakian N; Broad Institute of MIT and Harvard, Cambridge, MA, USA; Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA., Roignot J; Broad Institute of MIT and Harvard, Cambridge, MA, USA; Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA., Papagregoriou G; Molecular Medicine Research Center and Laboratory of Molecular and Medical Genetics, Department of Biological Sciences, University of Cyprus, Nicosia, Cyprus., Montesinos MS; Broad Institute of MIT and Harvard, Cambridge, MA, USA; Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA., Clark AR; Broad Institute of MIT and Harvard, Cambridge, MA, USA; Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA., McKinney D; Broad Institute of MIT and Harvard, Cambridge, MA, USA., Gutierrez J; Broad Institute of MIT and Harvard, Cambridge, MA, USA., Roth M; Broad Institute of MIT and Harvard, Cambridge, MA, USA., Ronco L; Broad Institute of MIT and Harvard, Cambridge, MA, USA., Elonga E; Broad Institute of MIT and Harvard, Cambridge, MA, USA., Carter TA; Broad Institute of MIT and Harvard, Cambridge, MA, USA., Gnirke A; Broad Institute of MIT and Harvard, Cambridge, MA, USA., Melanson M; Broad Institute of MIT and Harvard, Cambridge, MA, USA., Hartland K; Broad Institute of MIT and Harvard, Cambridge, MA, USA., Wieder N; Broad Institute of MIT and Harvard, Cambridge, MA, USA; Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA., Hsu JC; Broad Institute of MIT and Harvard, Cambridge, MA, USA., Deltas C; Molecular Medicine Research Center and Laboratory of Molecular and Medical Genetics, Department of Biological Sciences, University of Cyprus, Nicosia, Cyprus., Hughey R; Renal-Electrolyte Division, Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., Bleyer AJ; Section on Nephrology, Wake Forest School of Medicine, Medical Center Blvd., Winston-Salem, NC, USA; Research Unit for Rare Diseases, Department of Pediatrics and Adolescent Medicine, First Faculty of Medicine, Charles University, Prague, Czech Republic., Kmoch S; Section on Nephrology, Wake Forest School of Medicine, Medical Center Blvd., Winston-Salem, NC, USA; Research Unit for Rare Diseases, Department of Pediatrics and Adolescent Medicine, First Faculty of Medicine, Charles University, Prague, Czech Republic., Živná M; Research Unit for Rare Diseases, Department of Pediatrics and Adolescent Medicine, First Faculty of Medicine, Charles University, Prague, Czech Republic., Barešova V; Research Unit for Rare Diseases, Department of Pediatrics and Adolescent Medicine, First Faculty of Medicine, Charles University, Prague, Czech Republic., Kota S; Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA., Schlondorff J; Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA., Heiman M; Broad Institute of MIT and Harvard, Cambridge, MA, USA; The Picower Institute for Learning and Memory, Department of Brain and Cognitive Sciences, MIT, Cambridge, MA, USA., Alper SL; Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA., Wagner F; Broad Institute of MIT and Harvard, Cambridge, MA, USA., Weins A; Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA; Department of Pathology, Brigham and Women's Hospital, Boston, MA, USA., Golub TR; Broad Institute of MIT and Harvard, Cambridge, MA, USA., Lander ES; Broad Institute of MIT and Harvard, Cambridge, MA, USA; Department of Biology, MIT, Cambridge, MA, USA; Department of Systems Biology, Harvard Medical School, Boston, MA, USA., Greka A; Broad Institute of MIT and Harvard, Cambridge, MA, USA; Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA. Electronic address: agreka@broadinstitute.org.

Publikováno v: Cell [Cell] 2019 Jul 25; Vol. 178 (3), pp. 521-535.e23.

High-affinity accumulation of a maytansinoid in cells via weak tubulin interaction.

Autor: Goldmacher VS; Department of Cell Biology, ImmunoGen, Inc., Waltham, Massachusetts, United States of America., Audette CA; Department of Cell Biology, ImmunoGen, Inc., Waltham, Massachusetts, United States of America., Guan Y; Department of Systems Biology, Harvard Medical School, Boston, Massachusetts, United States of America; Renal Division, Brigham and Women's Hospital, Boston, Massachusetts, United States of America., Sidhom EH; Department of Systems Biology, Harvard Medical School, Boston, Massachusetts, United States of America; Renal Division, Brigham and Women's Hospital, Boston, Massachusetts, United States of America., Shah JV; Department of Systems Biology, Harvard Medical School, Boston, Massachusetts, United States of America; Renal Division, Brigham and Women's Hospital, Boston, Massachusetts, United States of America., Whiteman KR; Department of Cell Biology, ImmunoGen, Inc., Waltham, Massachusetts, United States of America., Kovtun YV; Department of Cell Biology, ImmunoGen, Inc., Waltham, Massachusetts, United States of America.

Publikováno v: PloS one [PLoS One] 2015 Feb 11; Vol. 10 (2), pp. e0117523. Date of Electronic Publication: 2015 Feb 11 (Print Publication: 2015).