Analysis of SPI-1 Dependent Type III Secretion and Injection Using a NanoLuc Luciferase-Based Assay

Autor: Sara Vilela, Pais, Sibel, Westerhausen, Erwin, Bohn, Samuel, Wagner

Publikováno v: Methods in molecular biology (Clifton, N.J.). 2427

Studies of bacterial protein secretion have relied on a variety of reporters that allow the tracking of secreted proteins. However, the lack of truly quantitative and highly sensitive reporters has hindered, in particular, the investigation of the ki

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::535b5d3bed82e5c9ca23f46f813807c6
https://pubmed.ncbi.nlm.nih.gov/35619025

Inhibition of autotransporter biogenesis by small molecules

Autor: Wouter S. P. Jong, Nicole N. van der Wel, H. Bart van den Berg van Saparoea, Sabrina M. de Munnik, Sebastiaan Kuhne, Maikel Wijtmans, Abdallah M. Abdallah, Peter van Ulsen, Sibel Westerhausen, Maurice Steenhuis, Samuel Wagner, Joen Luirink, Geert Jan Sterk

Publikováno v: Molecular microbiology, 112(1), 81-98. Wiley-Blackwell
Molecular Microbiology, 112(1), 81-98. Wiley-Blackwell
Steenhuis, M, Abdallah, A M, de Munnik, S M, Kuhne, S, Sterk, G J, van den Berg van Saparoea, B, Westerhausen, S, Wagner, S, van der Wel, N N, Wijtmans, M, van Ulsen, P, Jong, W S P & Luirink, J 2019, ' Inhibition of autotransporter biogenesis by small molecules ', Molecular Microbiology, vol. 112, no. 1, pp. 81-98 . https://doi.org/10.1111/mmi.14255
Molecular Microbiology

Summary Disarming pathogens by targeting virulence factors is a promising alternative to classic antibiotics. Many virulence factors in Gram‐negative bacteria are secreted via the autotransporter (AT) pathway, also known as Type 5 secretion. These

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c61046999d649c8df1b14bd2eb48abbb
https://research.vu.nl/en/publications/44577a99-e30a-4071-8b18-99c21eb19bf1

Bacterial type III secretion systems: a complex device for the delivery of bacterial effector proteins into eukaryotic host cells

Autor: Silke Malmsheimer, Iwan Grin, Claudia E Torres-Vargas, Samuel Wagner, Nidhi Singh, Sibel Westerhausen

Publikováno v: FEMS microbiology letters. 365(19)

Virulence-associated type III secretion systems (T3SS) serve the injection of bacterial effector proteins into eukaryotic host cells. They are able to secrete a great diversity of substrate proteins in order to modulate host cell function, and have e

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::da4b28b2b5080ec3bf867ed9cb7f9c9f
https://pubmed.ncbi.nlm.nih.gov/30107569