Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Si-Ang Li"'
Autor:
Juan-Juan Zhao, Xin-Yu Sun, Sai-Ning Tian, Zong-Ze Zhao, Meng-Di Yin, Mei Zhao, Feng Zhang, Si-Ang Li, Zhi-Xue Yang, Wei Wen, Tao Cheng, An Gong, Jian-Ping Zhang, Xiao-Bing Zhang
Publikováno v:
BMC Genomics, Vol 25, Iss 1, Pp 1-23 (2024)
Abstract Background CRISPR-Cas9 technology has advanced in vivo gene therapy for disorders like hemophilia A, notably through the successful targeted incorporation of the F8 gene into the Alb locus in hepatocytes, effectively curing this disorder in
Externí odkaz:
https://doaj.org/article/6b00ee3c16a94b60a7273e094f61df57
Autor:
Zi-Jun Quan, Si-Ang Li, Zhi-Xue Yang, Juan-Juan Zhao, Guo-Hua Li, Feng Zhang, Wei Wen, Tao Cheng, Xiao-Bing Zhang
Publikováno v:
Genomics, Proteomics & Bioinformatics, Vol 21, Iss 6, Pp 1221-1236 (2023)
To achieve the enormous potential of gene-editing technology in clinical therapies, one needs to evaluate both the on-target efficiency and unintended editing consequences comprehensively. However, there is a lack of a pipelined, large-scale, and eco
Externí odkaz:
https://doaj.org/article/420cff9498ce4ae0b14827b32d3370f6
Autor:
Zhi-Xue Yang, Ya-Wen Fu, Juan-Juan Zhao, Feng Zhang, Si-Ang Li, Mei Zhao, Wei Wen, Lei Zhang, Tao Cheng, Jian-Ping Zhang, Xiao-Bing Zhang
Publikováno v:
Genomics, Proteomics & Bioinformatics, Vol 21, Iss 6, Pp 1206-1220 (2023)
A series of clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR associated protein 9 (Cas9) systems have been engineered for genome editing. The most widely used Cas9 is SpCas9 from Streptococcus pyogenes and SaCas9 from Staphyl
Externí odkaz:
https://doaj.org/article/dd9a845d89064b8db6bec0665d9eb342
Publikováno v:
Poultry Science, Vol 103, Iss 9, Pp 104024- (2024)
ABSTRACT: Toxoplasma gondii (T. gondii) is an intracellular protozoan that can cause toxoplasmosis in all warm-blooded hosts. This study focused on the prevalence and genetic characterize of T. gondii in ducks from Fujian province, China. Genomic DNA
Externí odkaz:
https://doaj.org/article/9b4345a0807e4d77a10d71b762acf29b
Autor:
Meng-Jie Chu, Li-Yuan Huang, Wen-Yuan Miao, Ya-Fei Song, Ying-Sheng Lin, Si-Ang Li, Dong-Hui Zhou
Publikováno v:
Pathogens, Vol 12, Iss 10, p 1243 (2023)
Toxoplasma gondii is an opportunistic pathogenic protozoan that can infect all nucleated cells in almost all warm-blooded animals, including humans. T. gondii infection has been reported in many food animals worldwide. However, the prevalence and gen
Externí odkaz:
https://doaj.org/article/4db10d6a253b45278638b0be20aa03a9
Autor:
Wei Wen, Zi-Jun Quan, Si-Ang Li, Zhi-Xue Yang, Ya-Wen Fu, Feng Zhang, Guo-Hua Li, Mei Zhao, Meng-Di Yin, Jing Xu, Jian-Ping Zhang, Tao Cheng, Xiao-Bing Zhang
Publikováno v:
Genome Biology, Vol 22, Iss 1, Pp 1-22 (2021)
Abstract Background After repairing double-strand breaks (DSBs) caused by CRISPR-Cas9 cleavage, genomic damage, such as large deletions, may have pathogenic consequences. Results We show that large deletions are ubiquitous but are dependent on editin
Externí odkaz:
https://doaj.org/article/df51a80f1e864427bde4af68b40acd79
Autor:
Feng Zhang, Tao Cheng, Jing Xu, Guo-Hua Li, Juan-Juan Zhao, Mei Zhao, Xiao-Bing Zhang, Meng-Di Yin, Yi-Dan Sun, Xin-Yu Sun, Beldon Zhang, Jian-Ping Zhang, Fei-Ying Meng, Si-Ang Li
Publikováno v:
Human Gene Therapy. 33:404-420
Hemophilia A (HA) is a monogenic disease characterized by plasma clotting factor 8 (F8) deficiency due to F8 mutation. We have been attempting to cure HA permanently using a CRISPR-Cas9 gene-editing strategy. Here, we induced targeted integration of
Autor:
Juan Fu, Ya-Wen Fu, Juan-Juan Zhao, Zhi-Xue Yang, Si-Ang Li, Guo-Hua Li, Zi-Jun Quan, Feng Zhang, Jian-Ping Zhang, Xiao-Bing Zhang, Chang-Kai Sun
Publikováno v:
Stem Cell Reviews and Reports. 18:1822-1833
Highly efficient gene knockout (KO) editing of CRISPR–Cas9 has been achieved in iPSCs, whereas homology-directed repair (HDR)-mediated precise gene knock-in (KI) and high-level expression are still bottlenecks for the clinical applications of iPSCs
Autor:
Jing Xu, Mei Zhao, Wei Wen, Meng-Di Yin, Zi-Jun Quan, Tao Cheng, Ya-Wen Fu, Guo-Hua Li, Feng Zhang, Xiao-Bing Zhang, Zhi-Xue Yang, Jian-Ping Zhang, Si-Ang Li
Publikováno v:
Genome Biology, Vol 22, Iss 1, Pp 1-22 (2021)
Genome Biology
Genome Biology
BackgroundAfter repairing double-strand breaks (DSBs) caused by CRISPR-Cas9 cleavage, genomic damage, such as large deletions, may have pathogenic consequences.ResultsWe show that large deletions are ubiquitous but are dependent on editing sites and
Autor:
Zi-Jun Quan, Si-Ang Li, Zhi-Xue Yang, Juan-Juan Zhao, Guo-Hua Li, Feng Zhang, Wei Wen, Tao Cheng, Xiao-Bing Zhang
Publikováno v:
Genomics, proteomicsbioinformatics.
To achieve the enormous potential of gene-editing technology in clinical therapies, both the on-target and unintended editing consequences need to be thoroughly evaluated. However, there is a lack of a comprehensive, pipelined, large-scale and econom