Výsledky vyhledávání - "Shyamal P. Choudhari"

Identification of Excipients for Stabilizing Fiberless Adenovirus as Biopharmaceuticals

Autor: Nicholas Flynn, Joshua D. Ramsey, Grit Kupgan, Sravanthi Vupputuri, Shyamal P. Choudhari, William D. Picking, Wendy L. Picking, Adane S. Nigatu

Publikováno v: Journal of Pharmaceutical Sciences. 106:1764-1771

Reducing the promiscuous tropism of native adenovirus by using fiberless adenovirus is advantageous toward its use as a gene therapy vector or vaccine component. The removal of the fiber protein on native adenovirus abrogates several undesirable inte

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8b649532c8a03df84cffe2d9560a2046
https://doi.org/10.1016/j.xphs.2017.04.013

Zobrazit plný text záznamu

Biophysical Characterization of the Type III Secretion System Translocator Proteins and the Translocator Proteins Attached to Bacterium-Like Particles

Autor: C. Russell Middaugh, Maarten L. van Roosmalen, Jae Hyun Kim, Wendy L. Picking, Ronald T. Toth, Prashant Kumar, Kees Leenhouts, William D. Picking, Shyamal P. Choudhari, Xiaotong Chen

Publikováno v: Journal of Pharmaceutical Sciences. 104:4065-4073

Diarrhea caused by Shigella, Salmonella, and Yersinia is an important public health problem, but development of safe and effective vaccines against such diseases is challenging. A new antigen delivery platform called bacterium-like particles (BLPs) w

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8a10e0dfc4d989acecdcdad2f9940f96
https://doi.org/10.1002/jps.24659

Zobrazit plný text záznamu

Biophysical Characterization of the Type III Secretion Tip Proteins and the Tip Proteins Attached to Bacterium-Like Particles

Autor: William D. Picking, Xiaotong Chen, Shyamal P. Choudhari, Jae Hyun Kim, Wendy L. Picking, Kees Leenhouts, Maarten L. van Roosmalen, C. Russell Middaugh, Sangeeta B. Joshi, Jamie C. Greenwood

Publikováno v: Journal of Pharmaceutical Sciences. 104:424-432

Bacterium-like particles (BLPs), derived from Lactococcus lactis , offer a self-adjuvanting delivery vehicle for subunit protein vaccines. Proteins can be specifically loaded onto the BLPs via a peptidoglycan anchoring (PA) domain. In this study, the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::193eb9cf3707d848b14ba567ca7bc65d
https://doi.org/10.1002/jps.24047

Zobrazit plný text záznamu

Evaluation of immunogenicity and protective efficacy of orally delivered Shigella type III secretion system proteins IpaB and IpaD

Autor: John D. Clements, Jovita Diaz-McNair, Shyamal P. Choudhari, Marcela F. Pasetti, Wendy L. Picking, Francisco J. Martinez-Becerra, Shannon J. Heine

Publikováno v: Vaccine. 31:2919-2929

Shigella spp. are food- and water-borne pathogens that cause shigellosis, a severe diarrheal and dysenteric disease that is associated with a high morbidity and mortality in resource-poor countries. No licensed vaccine is available to prevent shigell

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::de47afd8190894ebeff4e493e420b13e
https://doi.org/10.1016/j.vaccine.2013.04.045

Zobrazit plný text záznamu

Studies of the conformational stability of invasion plasmid antigen B from Shigella

Autor: Shyamal P. Choudhari, Wendy L. Picking, William D. Picking, Michael L. Barta, Jamie C. Greenwood, Ryan Kramer, Sangeeta B. Joshi, C. Russell Middaugh, Brian V. Geisbrecht

Publikováno v: Protein Science. 22:666-670

Shigella spp. are the causative agent of shigellosis, the second leading cause of diarrhea in children of ages 2–5. Despite many years of research, a protective vaccine has been elusive. We recently demonstrated that invasion plasmid antigens B and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::43dd79275006a29fbc51890876485341
https://doi.org/10.1002/pro.2249

Zobrazit plný text záznamu

Oligomeric states of the Shigella translocator protein IpaB provide structural insights into formation of the type III secretion translocon

Autor: Wendy L. Picking, Philip R. Adam, William D. Picking, Sangeeta B. Joshi, C. Russell Middaugh, Nicholas E. Dickenson, Ryan Kramer, Shyamal P. Choudhari

Publikováno v: Protein Science. 22:614-627

The Shigella flexneri Type III secretion system (T3SS) senses contact with human intestinal cells and injects effector proteins that promote pathogen entry as the first step in causing life threatening bacillary dysentery (shigellosis). The Shigella

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::1d68cfa4035f9f0d436d8ea7baa31c65
https://doi.org/10.1002/pro.2245

Zobrazit plný text záznamu

Production of Well-Characterized Virus-like Particles in an Escherichia coli-Based Expression Platform for Preclinical Vaccine Assessments

Autor: Shyamal P. Choudhari, Fei P. Gao, Newton Wahome, C. Russell Middaugh, David B. Volkin, Prem S. Thapa, Anne Cooper

Publikováno v: Vaccine Design ISBN: 9781493933884

In this chapter we demonstrate a method to produce virus-like particles (VLPs) from Escherichia coli. Standard bacterial protocols are used for the cloning, transformation, and expression of the protein subunits. A two-step protein purification metho

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::fefb7add7f02d41a647b7d58fe6d3c44
https://doi.org/10.1007/978-1-4939-3389-1_29

Zobrazit plný text záznamu

Shigella IpaB and IpaD displayed on L. lactis bacterium-like particles induce protective immunity in adult and infant mice

Autor: William C. Blackwelder, Kees Leenhouts, Maarten L. van Roosmalen, Marcela F. Pasetti, Shyamal P. Choudhari, Olga L. Franco-Mahecha, Shannon J. Heine, Xiaotong Chen, Wendy L. Picking

Publikováno v: Immunology and cell biology

Shigella spp. are among the enteric pathogens with the highest attributable incidence of moderate-to-severe diarrhea in children under 5 years of age living in endemic areas. There are no vaccines available to prevent this disease. In this work, we i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::973f586ca374cee529341d4a7310b017
https://pubmed.ncbi.nlm.nih.gov/26054727

Zobrazit plný text záznamu

A Systematic Approach Toward Stabilization of CagL, a Protein Antigen from Helicobacter pylori That Is a Candidate Subunit Vaccine

Autor: Shyamal P. Choudhari, Joshua D. Ramsey, William D. Picking, Thomas G. Blanchard, Kirk P. Pendleton

An important consideration in the development of subunit vaccines is the loss of activity caused by physical instability of the protein. Such instability often results from suboptimal solution conditions related to pH and temperature. Excipients can

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e52e8470914c299032d02b91d4d78b8b
https://europepmc.org/articles/PMC3903303/

Zobrazit plný text záznamu

Three functional β-carbonic anhydrases in Pseudomonas aeruginosa PAO1: role in survival in ambient air

Autor: Shyamal P. Choudhari, Wendy L. Picking, Shane Hnatusko, Marianna A. Patrauchan, Nicholas E. Dickenson, Shalaka R. Lotlikar

Publikováno v: Microbiology (Reading, England). 159(Pt 8)

Bacterial β-class carbonic anhydrases (CAs) are zinc metalloenzymes catalysing reversible hydration of CO2. They maintain the intracellular balance of CO2/bicarbonate required for biosynthetic reactions and represent a new group of antimicrobial dru

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4a568055938c3b0c81ed481e966542f2
https://pubmed.ncbi.nlm.nih.gov/23728627

Zobrazit plný text záznamu

Vyhledávací nástroje:

Upřesnit hledání