Zobrazeno 1 - 10
of 56
pro vyhledávání: '"Shunji Kaya"'
Publikováno v:
Journal of Biological Chemistry. 280:18736-18744
The highly conserved amino acids of rat Na,K-ATPase, Thr-774 in the transmembrane helices M5, Val-920 and Gln-923 in M8, and Glu-953 and Glu-954 in M9, the side chains of which appear to be in close proximity, were mutated, and the resulting proteins
Autor:
Kazuhiro Oiwa, Shunji Kaya, Toshiaki Imagawa, Yutaro Hayashi, Michio Yazawa, Kazuhiro Abe, Mahito Kikumoto, Kazuya Taniguchi, Tsuyoshi Katoh
Publikováno v:
Biochemistry. 42:15132-15138
Membrane-bound H/K-ATPase was solubilized by octaethylene glycol dodecyl ether (C(12)E(8)) or n-octyl glucoside (nOG). H/K-ATPase activity and the distribution of protomeric and oligomeric components were evaluated by high-performance gel chromatogra
Autor:
Motoi Kanagawa, Tadayasu Ohkubo, Keiichi Kawano, Shin-Ichiro Nishimura, Shunji Kaya, Kazuya Taniguchi, Katsutoshi Nitta, Tomoyasu Aizawa, Makoto Demura, Naoki Fujitani
Publikováno v:
Biochemical and Biophysical Research Communications. 300:223-229
It has been well established that phosphorylation is an important reaction for the regulation of protein functions. In the N-terminal domain of the alpha-chain of pig gastric H(+)/K(+)-ATPase, reversible sequential phosphorylation occurs at Tyr 10 an
Autor:
Masaharu Sumii, Masahiro Ohta, Masaharu Yoshihara, Shunji Kaya, Shinji Tanaka, Masanori Ito, Ken Haruma, Kazuaki Chayama, Hiroshi Masuda, Hirohisa Kai
Publikováno v:
Digestive Diseases and Sciences. 47:1080-1085
We investigated serum levels of anti-parietal cell antibody (APCA) in relation to various gastric diseases. Subjects were 224 Japanese patients including 58 with gastric cancer. All patients underwent gastroscopy, and APCA was investigated by enzyme-
Autor:
Masanori Ito, Tomoari Kamada, Shinji Tanaka, Masaharu Yoshihara, Atsunori Sasaki, Masaharu Sumii, Shunji Kaya, Kazuaki Chayama, Sun Jin Kim, Ken Haruma
Publikováno v:
Scandinavian Journal of Gastroenterology. 37:287-293
Helicobacter pylori plays an important part in the progression of atrophic gastritis; however, markers for predicting the progression of atrophic gastritis remain unidentified. We investigated the relation between the degree of atrophic gastritis and
Publikováno v:
ResearcherID
Since the discovery of Na/K-ATPase, evidence has accumulated to suggest that 1 mol of ATP hydrolysis occurs via the Na(+)-occluded ADP-sensitive phosphoenzyme, the K(+)-sensitive phosphoenzyme and the K(+)-occluded enzyme accompanying active transpor
Autor:
Akira Shimada, Shunji Kaya, Toshiaki Imagawa, Kazuya Taniguchi, Motoi Kanagawa, K Kikuchi, Katsuhiko Togawa, S. Watanabe
Publikováno v:
Journal of Biochemistry. 127:821-828
H/K-ATPase preparations (the G1 membrane) from pig stomach contain both kinases and phosphatases and show reversible phosphorylation of Tyr(7), Tyr(10), and Ser(27) residues of the alpha-chain of H/K-ATPase. The Tyr-kinase is sensitive to genistein a
Publikováno v:
Journal of Biological Chemistry. 273:24339-24345
The addition of ATP to Mg2+-Na+-bound-probe labeled Na+,K+-ATPase preparations containing ∼0.5 mol of pyridoxal 5′-diphospho-5′-adenosine (AP2PL) probe at Lys-480 and ∼0.9 mol of fluorescein 5′-isothiocyanate (FITC) probe at Lys-501 showed
Autor:
Akira Shimada, Yoshihiko Usida, Hiroaki Eguchi, Kazuya Taniguchi, Shunji Kaya, Yuka Ootomo, Ken'ichi Nomoto, Miki Kikuchi
Publikováno v:
Journal of Biochemistry. 122:659-665
H+,K+-ATPase preparations from pig stomach were modified with a sulfhydryl fluorescence reagent, N-[p-(2-benzimidazolyl)phenyl] maleimide (BIPM). The addition of ATP to the modified enzyme preparations in the presence of Mg2+ decreased the BIPM fluor
Autor:
Akira Shimada, Tomomi Ishiguro, Toshiaki Imagawa, Kazuya Taniguchi, Katsuhiko Togawa, Shunji Kaya
Publikováno v:
Journal of Biological Chemistry. 270:15475-15478
When pig stomach membrane H+,K(+)-ATPase preparations were incubated with [gamma-32P]ATP and Mg2+ with vanadate, 32P was incorporated into the alpha-chain of H+,K(+)-ATPase to a steady-state level of approximately 0.7 mol of phosphotyrosine (Tyr(P))/