Zobrazeno 1 - 10
of 38
pro vyhledávání: '"Shuen Hon"'
Autor:
Daniela Prates Chiarelli, Bishal Dev Sharma, Shuen Hon, Luana Walravens Bergamo, Lee R. Lynd, Daniel G. Olson
Publikováno v:
Metabolic Engineering Communications, Vol 19, Iss , Pp e00243- (2024)
Clostridium thermocellum is a thermophilic anaerobic bacterium that could be used for cellulosic biofuel production due to its strong native ability to consume cellulose, however its ethanol production ability needs to be improved to enable commercia
Externí odkaz:
https://doaj.org/article/899bd085040646c9a3329da067c31665
Autor:
Daniel G. Olson, Marybeth I. Maloney, Anthony A. Lanahan, Nicholas D. Cervenka, Ying Xia, Angel Pech-Canul, Shuen Hon, Liang Tian, Samantha J. Ziegler, Yannick J. Bomble, Lee R. Lynd
Publikováno v:
Biotechnology for Biofuels and Bioproducts, Vol 16, Iss 1, Pp 1-15 (2023)
Abstract Clostridium thermocellum is a natively cellulolytic bacterium that is promising candidate for cellulosic biofuel production, and can produce ethanol at high yields (75–80% of theoretical) but the ethanol titers produced thus far are too lo
Externí odkaz:
https://doaj.org/article/5aaf12444d4d4f58bc14ee1dfb715d71
Autor:
Shuen Hon, Evert K. Holwerda, Robert S. Worthen, Marybeth I. Maloney, Liang Tian, Jingxuan Cui, Paul P. Lin, Lee R. Lynd, Daniel G. Olson
Publikováno v:
Biotechnology for Biofuels, Vol 11, Iss 1, Pp 1-11 (2018)
Abstract Background Clostridium thermocellum has been the subject of multiple metabolic engineering strategies to improve its ability to ferment cellulose to ethanol, with varying degrees of success. For ethanol production in C. thermocellum, the con
Externí odkaz:
https://doaj.org/article/d1e984c30784466da8f96b19ca962af0
Autor:
Liang Tian, Skyler J. Perot, Shuen Hon, Jilai Zhou, Xiaoyu Liang, Jason T. Bouvier, Adam M. Guss, Daniel G. Olson, Lee R. Lynd
Publikováno v:
Microbial Cell Factories, Vol 16, Iss 1, Pp 1-10 (2017)
Abstract Background Pyruvate decarboxylase (PDC) is a well-known pathway for ethanol production, but has not been demonstrated for high titer ethanol production at temperatures above 50 °C. Result Here we examined the thermostability of eight PDCs.
Externí odkaz:
https://doaj.org/article/b56636ddd46e4e82a217d45ab59ee148
Autor:
Shuen Hon, Anthony A. Lanahan, Liang Tian, Richard J. Giannone, Robert L. Hettich, Daniel G. Olson, Lee R. Lynd
Publikováno v:
Metabolic Engineering Communications, Vol 3, Iss , Pp 120-129 (2016)
Clostridium thermocellum is a promising candidate for ethanol production from cellulosic biomass, but requires metabolic engineering to improve ethanol yield. A key gene in the ethanol production pathway is the bifunctional aldehyde and alcohol dehyd
Externí odkaz:
https://doaj.org/article/ec0ed89644f44e0b9d258604e81fdd4b
Autor:
Daniel G. Olson, Marybeth Maloney, Anthony A. Lanahan, Shuen Hon, Loren J. Hauser, Lee R. Lynd
Publikováno v:
Metabolic Engineering Communications, Vol 2, Iss , Pp 23-29 (2015)
A key tool for metabolic engineering is the ability to express heterologous genes. One obstacle to gene expression in non-model organisms, and especially in relatively uncharacterized bacteria, is the lack of well-characterized promoters. Here we tes
Externí odkaz:
https://doaj.org/article/4e77930198a348569cc263c7a75ba496
Autor:
Shuen, Hon, Tyler, Jacobson, David M, Stevenson, Marybeth I, Maloney, Richard J, Giannone, Robert L, Hettich, Daniel, Amador-Noguez, Daniel G, Olson, Lee R, Lynd
Publikováno v:
Appl Environ Microbiol
Glycolysis is an ancient, widespread, and highly conserved metabolic pathway that converts glucose into pyruvate. In the canonical pathway, the phosphofructokinase (PFK) reaction plays an important role in controlling flux through the pathway. Clostr
Autor:
Shuen Hon, Tyler Jacobson, David M. Stevenson, Marybeth I. Maloney, Richard J. Giannone, Robert L. Hettich, Daniel Amador-Noguez, Daniel G. Olson, Lee R. Lynd
Publikováno v:
Applied and Environmental Microbiology. 88
The ability to control the distribution of thermodynamic driving force throughout a metabolic pathway is likely to be an important tool for metabolic engineering. The phosphofructokinase reaction is a key enzyme in Embden-Mayerhof-Parnas glycolysis a
Autor:
Daniel G. Olson, Shuen Hon, Jeroen Girwar Koendjbiharie, Richard van Kranenburg, David M. Stevenson, Daniel Amador-Noguez, Martin Pabst, Jingxuan Cui, Lee R. Lynd, Robert Hooftman
Publikováno v:
Journal of Biological Chemistry, 295(7), 1867-1878
Journal of Biological Chemistry 295 (2020) 7
The Journal of Biological Chemistry
Journal of Biological Chemistry, 295(7)
Journal of Biological Chemistry 295 (2020) 7
The Journal of Biological Chemistry
Journal of Biological Chemistry, 295(7)
The genomes of most cellulolytic clostridia do not contain genes annotated as transaldolase. Therefore, for assimilating pentose sugars or for generating C5 precursors (such as ribose) during growth on other (non-C5) substrates, they must possess a p
Autor:
Teun, Kuil, Shuen, Hon, Johannes, Yayo, Charles, Foster, Giulia, Ravagnan, Costas D, Maranas, Lee R, Lynd, Daniel G, Olson, Antonius J A, van Maris
Publikováno v:
Applied and environmental microbiology. 88(4)
The atypical glycolysis of Clostridium thermocellum is characterized by the use of pyrophosphate (PP