Zobrazeno 1 - 10
of 188
pro vyhledávání: '"Shozaburo KITAOKA"'
Publikováno v:
Bioscience, biotechnology, and biochemistry. 57(2)
(1993). Enriching Euglena with Unsaturated Fatty Acids. Bioscience, Biotechnology, and Biochemistry: Vol. 57, No. 2, pp. 352-353.
Autor:
Maki Kondo, Kazutaka Miyatake, Hiroshi Inui, Kouki Ono, Shozaburo Kitaoka, Tetsuaki Osafune, Yoshihisa Nakano, Mikio Nishimura
Publikováno v:
The Journal of Eukaryotic Microbiology. 50:92-96
Isocitrate lyase and malate synthase are specific enzymes of the glyoxylate cycle, used here as glyoxysomal markers. Both enzymes were found in the mitochondrial fraction after organelle fractionation by isopycnic centrifugation. Electron microscopy
Autor:
Yuji Isegawa, Hiroshi Inui, Yoshihisa Nakano, Kazutaka Miyatake, Osamu Ohya, Shozaburo Kitaoka
Publikováno v:
The Journal of Eukaryotic Microbiology. 43:177-180
Euglena gracilis requires cobalamin (Cbl) as an essential growth factor. Phosphatidylcholine (PC) synthesis was greatly reduced by Cbl deficiency. Rapid cell division occurred after Cbl was replenished, and PC was actively synthesized during the cell
Autor:
Yoshihisa Nakano, Masako Sakamoto, Tsutomu Masuda, Shozaburo Kitaoka, Yoshinori Tanigawa, Yukio Yanagimoto
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 60:1941-1944
Mitochondrial serine hydroxymethyltransferase, l-serine: tetrahydrofolate 5,10-hydroxymethyl-transferase (EC 2.1.2.1), (m-SHMT) was extracted and highly purified from Euglena gracilis z. The specific activity increased from the crude extract with 10%
Autor:
Shozaburo Kitaoka
Publikováno v:
Trends in Glycoscience and Glycotechnology. 7:351-364
Publikováno v:
Journal of Japan Oil Chemists' Society. 44:640-646
The effects of pH shift in the stationary phase of growth on the incorporation of exogenous docosahexaenoic acid (DHA) into Euglena cells and lipid classes were examined. At pH 6.57.5 the incorporation of DHA was maximum, and content of DHA in the ce
Autor:
Kazutaka Miyatake, Hiroshi Inui, Kouki Ono, Masahiro Okihashi, Shozaburo Kitaoka, Yoshihisa Nakano
Publikováno v:
The Journal of Eukaryotic Microbiology. 41:536-539
Isocitrate lyase was purified to homogeneity from ethanol-grown Euglena gracilis. The specific activity was 0.26 μmol/ min/mg protein. The molecular mass of the enzyme was calculated to be 380 kDa by gel filtration on a Superose 6 column. The subuni
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 58:1964-1967
Effects of pH shifted in the stationary phase on the chemical composition of Euglena gracilis cells were examined. When the cells that were cultivated at fixed pH of 4.5 reached stationary phase, the pH of the culture was shifted to 2.0–8.5, and th
Publikováno v:
NIPPON SUISAN GAKKAISHI. 59:1051-1058
Since Euglena gracilis incorporate and accumulate n-3 highly unsaturated fatty acids (n-3 HUFA), the fatty acid composition of the cells can be controlled by supplementation of the fatty acids into growth media. Rotifers and Artemia were enriched wit
Purification and Characterization of Cytosolic Serine Hydroxymethyltransferase fromEuglena gracilisz
Publikováno v:
Agricultural and Biological Chemistry. 55:2243-2249