Precise and simultaneous quantification of mitochondrial DNA heteroplasmy and copy number by digital PCR

Autor: Shoop, Wendy K., Gorsuch, Cassandra L., Bacman, Sandra R., Moraes, Carlos T.

Publikováno v: In Journal of Biological Chemistry November 2022 298(11)

Efficient elimination of MELAS-associated m.3243G mutant mitochondrial DNA by an engineered mitoARCUS nuclease.

Autor: Shoop WK; Precision BioSciences, Durham, NC, USA.; Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine, Miami, FL, USA., Lape J; Precision BioSciences, Durham, NC, USA., Trum M; Precision BioSciences, Durham, NC, USA., Powell A; Precision BioSciences, Durham, NC, USA., Sevigny E; Precision BioSciences, Durham, NC, USA., Mischler A; Precision BioSciences, Durham, NC, USA., Bacman SR; Department of Neurology, University of Miami Miller School of Medicine, Miami, FL, USA., Fontanesi F; Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine, Miami, FL, USA., Smith J; Precision BioSciences, Durham, NC, USA., Jantz D; Precision BioSciences, Durham, NC, USA., Gorsuch CL; Precision BioSciences, Durham, NC, USA. cassie.gorsuch@precisionbiosciences.com., Moraes CT; Department of Neurology, University of Miami Miller School of Medicine, Miami, FL, USA. CMoraes@med.miami.edu.

Publikováno v: Nature metabolism [Nat Metab] 2023 Dec; Vol. 5 (12), pp. 2169-2183. Date of Electronic Publication: 2023 Nov 30.