Zobrazeno 1 - 10
of 34
pro vyhledávání: '"Shoko Shinya"'
Multi-functionality of a tryptophan residue conserved in substrate-binding groove of GH19 chitinases
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
Abstract GH19 and GH22 glycoside hydrolases belonging to the lysozyme superfamily have a related structure/function. A highly conserved tryptophan residue, Trp103, located in the binding groove of a GH19 chitinase from moss Bryum coronatum (BcChi-A)
Externí odkaz:
https://doaj.org/article/af857347caee45848bbbbf057bade540
Autor:
Natsuki Fukuda, Kentaro Noi, Lidong Weng, Yoshihiro Kobashigawa, Hiromi Miyazaki, Yukari Wakeyama, Michiyo Takaki, Yusuke Nakahara, Yuka Tatsuno, Makiyo Uchida-Kamekura, Yoshiaki Suwa, Takashi Sato, Naoki Ichikawa-Tomikawa, Motoyoshi Nomizu, Yukio Fujiwara, Fumina Ohsaka, Takashi Saitoh, Katsumi Maenaka, Hiroyuki Kumeta, Shoko Shinya, Chojiro Kojima, Teru Ogura, Hiroshi Morioka
Publikováno v:
Molecules, Vol 22, Iss 10, p 1695 (2017)
Due to their lower production cost compared with monoclonal antibodies, single-chain variable fragments (scFvs) have potential for use in several applications, such as for diagnosis and treatment of a range of diseases, and as sensor elements. Howeve
Externí odkaz:
https://doaj.org/article/ec91204768074e62bae41965348e2ac8
Autor:
Ken‐ichiro Taoka, Ikumi Kawahara, Shoko Shinya, Ken‐ichi Harada, Eiki Yamashita, Zenpei Shimatani, Kyoko Furuita, Tomoaki Muranaka, Tokitaka Oyama, Rie Terada, Atsushi Nakagawa, Toshimichi Fujiwara, Hiroyuki Tsuji, Chojiro Kojima
Publikováno v:
The Plant Journal. 112:1337-1349
Structure-based high-throughput screening of chemical compounds that target protein-protein interactions (PPIs) is a promising technology for gaining insight into how plant development is regulated, leading to many potential agricultural applications
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::992ce178351461fd77d824653e1a9f70
https://doi.org/10.1111/tpj.16008
https://doi.org/10.1111/tpj.16008
Autor:
Shoko Shinya, Ritsuko Katahira, Kyoko Furuita, Toshihiko Sugiki, Young-Ho Lee, Yoshikazu Hattori, Kohei Takeshita, Atsushi Nakagawa, Aoi Kokago, Ken-ichi Akagi, Muneki Oouchi, Fumiaki Hayashi, Takanori Kigawa, Midori Takimoto-Kamimura, Toshimichi Fujiwara, Chojiro Kojima
Publikováno v:
RSC Medicinal Chemistry. 13:1100-1111
19F-NMR analysis using the optimized 19F chemical library enables the modeling of the structure of the weakly bound protein–compound complex, overcoming the difficulty in fragment-based drug discovery.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8a4bd97cf852c69003c817b3f14ba2ec
https://doi.org/10.1039/d2md00170e
https://doi.org/10.1039/d2md00170e
Autor:
Shoko, Shinya, Ritsuko, Katahira, Kyoko, Furuita, Toshihiko, Sugiki, Young-Ho, Lee, Yoshikazu, Hattori, Kohei, Takeshita, Atsushi, Nakagawa, Aoi, Kokago, Ken-ichi, Akagi, Muneki, Oouchi, Fumiaki, Hayashi, Takanori, Kigawa, Midori, Takimoto-Kamimura, Toshimichi, Fujiwara, Chojiro, Kojima
Publikováno v:
RSC Medicinal Chemistry.
Fragment-based drug discovery (FBDD), which involves small compounds
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=jairo_______::c3830ebf3051385a4b8f25201d1760e7
http://hdl.handle.net/10131/00014763
http://hdl.handle.net/10131/00014763
Multi-functionality of a tryptophan residue conserved in substrate-binding groove of GH19 chitinases
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
Scientific Reports
Scientific Reports
GH19 and GH22 glycoside hydrolases belonging to the lysozyme superfamily have a related structure/function. A highly conserved tryptophan residue, Trp103, located in the binding groove of a GH19 chitinase from moss Bryum coronatum (BcChi-A) appears t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b8036ad0f53b3a1b236df89749bc5b5
https://doi.org/10.1038/s41598-021-81903-3
https://doi.org/10.1038/s41598-021-81903-3
Publikováno v:
International Journal of Biological Macromolecules. 104:1633-1640
We review studies on biochemical characterization of the structures and functions of chitinase, chitosanase, and chitobiase produced by cells of the bacterium, Paenibacillus sp. IK-5. The IK-5 chitinases comprise two GH18 chitinases (ChiA and ChiB),
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4635954c1717230dfd74ea5c4ca701dc
https://doi.org/10.1016/j.ijbiomac.2017.04.118
https://doi.org/10.1016/j.ijbiomac.2017.04.118
Autor:
Chojiro Kojima, Ryszard Brzezinski, Mariana Gabriela Ghinet, Kyoko Furuita, Tamo Fukamizo, Sneha Shah, Evgenii L. Kovrigin, Shoko Shinya
Publikováno v:
Journal of Biomolecular NMR. 67:309-319
Chitosan interaction with chitosanase was examined through analysis of spectral line shapes in the NMR HSQC titration experiments. We established that the substrate, chitosan hexamer, binds to the enzyme through the three-state induced-fit mechanism
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c4d20a782571576589f9a35fb038112a
https://doi.org/10.1007/s10858-017-0109-6
https://doi.org/10.1007/s10858-017-0109-6
Autor:
Siqin Bala, Chojiro Kojima, Arpita Srivastava, Florence Tama, Shoko Shinya, Naohiro Kobayashi, Daisuke Kohda, Osamu Miyashita, Atsushi Shimada, Marie Ishikawa
Publikováno v:
Biochimica et biophysica acta. General subjects. 1864(2)
Background In protein crystals, flexible loops are frequently deformed by crystal contacts, whereas in solution, the large motions result in the poor convergence of such flexible loops in NMR structure determinations. We need an experimental techniqu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::12df09ce6411b0083cd1d43c876767ac
https://pubmed.ncbi.nlm.nih.gov/31449839
https://pubmed.ncbi.nlm.nih.gov/31449839
Autor:
Tamo Fukamizo, Shoko Shinya
Publikováno v:
Advances in Experimental Medicine and Biology ISBN: 9789811373176
Plant chitinase hydrolyzing β-1,4-glycosidic linkages of chitin are major enzymes acting in plant-microbe interactions and are involved in self-defense against fungal pathogens. Chitosanases from soil bacteria are also involved in plant defense by h
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f248b3b273aabfbbfa5b09869341b183
https://doi.org/10.1007/978-981-13-7318-3_12
https://doi.org/10.1007/978-981-13-7318-3_12
