Zobrazeno 1 - 10
of 114
pro vyhledávání: '"Shoji Mano"'
Publikováno v:
Scientific Reports, Vol 13, Iss 1, Pp 1-11 (2023)
Abstract The demand for recombinant proteins is rising dramatically, and effective production systems are currently being developed. The production of recombinant proteins in plants is a promising approach due to its low cost and low risk of contamin
Externí odkaz:
https://doaj.org/article/3991c741790f4ece84421aab0e764d2e
Autor:
Kazusato Oikawa, Shino Goto-Yamada, Yasuko Hayashi, Daisuke Takahashi, Yoshitaka Kimori, Michitaro Shibata, Kohki Yoshimoto, Atsushi Takemiya, Maki Kondo, Kazumi Hikino, Akira Kato, Keisuke Shimoda, Haruko Ueda, Matsuo Uemura, Keiji Numata, Yoshinori Ohsumi, Ikuko Hara-Nishimura, Shoji Mano, Kenji Yamada, Mikio Nishimura
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-17 (2022)
Pexophagy plays a pivotal role in the selective removal of ROS-generating peroxisomes, which protects plants from oxidative damage during photosynthesis under high-intensity light.
Externí odkaz:
https://doaj.org/article/7e185e55c37f4471b2b89b2fbc5773d4
Autor:
Shino Goto-Yamada, Kazusato Oikawa, Katsuyuki T. Yamato, Masatake Kanai, Kazumi Hikino, Mikio Nishimura, Shoji Mano
Publikováno v:
Frontiers in Cell and Developmental Biology, Vol 10 (2022)
Peroxisomes are present in eukaryotic cells and have essential roles in various biological processes. Plant peroxisomes proliferate by de novo biosynthesis or division of pre-existing peroxisomes, degrade, or replace metabolic enzymes, in response to
Externí odkaz:
https://doaj.org/article/13eaf94135c747e0947551c7a2b0dadd
Autor:
Shino Goto-Yamada, Kazusato Oikawa, Jakub Bizan, Shuji Shigenobu, Katsushi Yamaguchi, Shoji Mano, Makoto Hayashi, Haruko Ueda, Ikuko Hara-Nishimura, Mikio Nishimura, Kenji Yamada
Publikováno v:
Frontiers in Plant Science, Vol 10 (2019)
Autophagy is an essential system for degrading and recycling cellular components for survival during starvation conditions. Under sucrose starvation, application of a papain protease inhibitor E-64d to the Arabidopsis root and tobacco BY-2 cells indu
Externí odkaz:
https://doaj.org/article/9c2b6725e16b4a9aa28ac147dbf766e2
Publikováno v:
PLoS ONE, Vol 14, Iss 11, p e0224795 (2019)
Ubiquitin-like proteins play important roles in the regulation of many biological processes. UBL5 (Ubiquitin-like protein 5)/Hub1 (Homologous to ubiquitin 1), a member of the ubiquitin family, acts as a ubiquitin-like modifier on a specific target, t
Externí odkaz:
https://doaj.org/article/40cddadbc0bd4bbda362de19e6ae2b35
Autor:
Shoji Mano, Ryuichi Nishihama, Sakiko Ishida, Kazumi Hikino, Maki Kondo, Mikio Nishimura, Katsuyuki T Yamato, Takayuki Kohchi, Tsuyoshi Nakagawa
Publikováno v:
PLoS ONE, Vol 13, Iss 10, p e0204964 (2018)
The liverwort Marchantia polymorpha is an emerging model species for basal lineage plant research. In this study, two Gateway cloning-compatible binary vector series, R4pMpGWB and R4L1pMpGWB, were generated to facilitate production of transgenic M. p
Externí odkaz:
https://doaj.org/article/d9836f85c40846f89bb22169f1dc3d39
Autor:
Mostafa Aboulela, Yuji Tanaka, Kohji Nishimura, Shoji Mano, Mikio Nishimura, Sumie Ishiguro, Tetsuya Kimura, Tsuyoshi Nakagawa
Publikováno v:
PLoS ONE, Vol 12, Iss 5, p e0177889 (2017)
Vast numbers of proteins work cooperatively to exert their functions in various cells. In order to understand the functions and molecular mechanisms of these proteins in plants, analyses of transgenic plants that concomitantly express two protein-cod
Externí odkaz:
https://doaj.org/article/82506aabf3064fc98ac0d21d1d252325
Autor:
Yoichiroh Hosokawa, Takanori Iino, Kazusato Oikawa, Shoji Mano, Kenji Yamada, Mikio Nishimura
Publikováno v:
Bio-Protocol, Vol 6, Iss 11 (2016)
This is the detailed protocol to quantify adhesion strength between peroxisomes and chloroplasts in plant leaf palisade mesophyll cells described by Oikawa et al. (2015). The quantification was performed by utilizing local explosion induced by focusi
Externí odkaz:
https://doaj.org/article/adda97bd0d0c46b89a877a0f4f1bb9d9
Publikováno v:
Bio-Protocol, Vol 6, Iss 8 (2016)
Quantitative analysis has been necessary for deeply understanding characteristic of organelles function. This is the detailed protocol for the quantification of the physical interaction between peroxisomes and chloroplasts taken by laser scanning mic
Externí odkaz:
https://doaj.org/article/1e7ee6e126b040fb99f3cbd36e393a51
Autor:
Akane Kamigaki, Kazumasa Nito, Kazumi Hikino, Shino Goto-Yamada, Mikio Nishimura, Tsuyoshi Nakagawa, Shoji Mano
Publikováno v:
PLoS ONE, Vol 11, Iss 8, p e0160717 (2016)
Bimolecular fluorescence complementation (BiFC) is widely used to detect protein-protein interactions, because it is technically simple, convenient, and can be adapted for use with conventional fluorescence microscopy. We previously constructed enhan
Externí odkaz:
https://doaj.org/article/2102ceeb9c0e4d80bdc2c47787b98b83