Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Shoji J Ohuchi"'
Publikováno v:
PLoS ONE, Vol 10, Iss 3, p e0120576 (2015)
An affinity resin-based pull-down method is convenient for the purification of biochemical materials. However, its use is difficult for the isolation of a molecular complex fully loaded with multiple components from a reaction mixture containing the
Externí odkaz:
https://doaj.org/article/2a3d290257c8490e9e436d5234814eb2
Publikováno v:
Biochemical and Biophysical Research Communications. 466:388-392
RNA-protein complexes (RNPs) are useful for constructing functional nano-objects because a variety of functional proteins can be displayed on a designed RNA scaffold. Here, we report circular permutations of an RNA-binding protein L7Ae based on the t
Publikováno v:
FEBS Letters. 589:2424-2428
Multifunctional molecular complexes are valuable tools with a variety of applications. We have developed an RNA–protein complex (RNP) containing three different proteins attached to the tips of a triangular RNA scaffold. We designed and constructed
Publikováno v:
PLoS ONE
PLoS ONE, Vol 10, Iss 3, p e0120576 (2015)
PLoS ONE, Vol 10, Iss 3, p e0120576 (2015)
An affinity resin-based pull-down method is convenient for the purification of biochemical materials. However, its use is difficult for the isolation of a molecular complex fully loaded with multiple components from a reaction mixture containing the
Publikováno v:
Journal of Biological Chemistry. 279:540-546
In this paper we report newly selected artificial modules that enhance the kcat values comparable with or higher than those of the wild-type ribozyme with broad substrate specificity. The elements required for the catalysis of Group I intron ribozyme
Autor:
Shoji J. Ohuchi
Publikováno v:
Methods in Molecular Biology ISBN: 9781627037549
Artificial riboswitches that respond to the concentrations of intracellular proteins are a promising tool with a variety of applications. They can be designed and engineered using existing RNA aptamers that target proteins. Aptamers are generated via
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8611d834357e5aba749ea34e0a6e3df2
https://doi.org/10.1007/978-1-62703-755-6_4
https://doi.org/10.1007/978-1-62703-755-6_4
Publikováno v:
Journal of biochemistry. 145(4)
Group I intron ribozymes have a modular architecture and structural elements essential for catalysis. The elements are located in the conserved modular domain P3-P7 that is stabilized by another conserved module, P4-P6. It has been reported that arti
Publikováno v:
Scopus-Elsevier
Catalysis of Group I intron ribozymes is carried out by its core region consisting of two helical domains P4-P6 and P3-P7. Recently, our laboratory showed that a mutant Group I ribozyme lacking both the P4-P6 domain and the base-triples can perform t
Publikováno v:
Scopus-Elsevier
All Group I intron ribozymes contain a conserved core region consisting of two helical domains, P4-P6 and P3-P7. Recent studies have demonstrated that the elements required for catalysis are concentrated in the P3-P7 domain. We carried out in vitro s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3e933038710ed1c656ad3a79b1782c3f
http://www.scopus.com/inward/record.url?eid=2-s2.0-0036682095&partnerID=MN8TOARS
http://www.scopus.com/inward/record.url?eid=2-s2.0-0036682095&partnerID=MN8TOARS