Výsledky vyhledávání - "Shiv Raj Tyagi"

Altered diacylglycerol level and metabolism in neutrophils from patients with localized juvenile periodontitis

Autor: David J. Uhlinger, J D Lambeth, T. E. Van Dyke, C. Champagne, Shiv Raj Tyagi

Publikováno v: Infection and Immunity. 60:2481-2487

Diacylglycerol, a physiological activator of protein kinase C, was elevated nearly twofold in unstimulated peripheral blood neutrophils from patients with localized juvenile periodontitis compared with cells from normal individuals. These cells also

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9487be4cab0ee99eae7705e55a9df91a
https://doi.org/10.1128/iai.60.6.2481-2487.1992

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Cyclic AMP-elevating agents block chemoattractant activation of diradylglycerol generation by inhibiting phospholipase D activation

Autor: S. C. Olson, D N Burnham, Shiv Raj Tyagi, J D Lambeth

Publikováno v: Journal of Biological Chemistry. 266:3498-3504

Agents which elevate cellular cAMP (prostaglandin E2, theophylline, and forskolin) or mimic cAMP action (dibutyryl cAMP) are known to inhibit human neutrophil activation (superoxide generation and secretion) by receptor-linked agonists such as formyl

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::8820667ccebb6fc1af050b06f4cbb43d
https://doi.org/10.1016/s0021-9258(19)67823-3

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Reconstitution and characterization of the human neutrophil respiratory burst oxidase using recombinant p47-phox, p67-phox and plasma membrane

Autor: Shiv Raj Tyagi, David J. Uhlinger, Mary L. Kreck, Nickolas Neckelmann, J. David Lambeth, K. Leigh Inge

Publikováno v: Biochemical and biophysical research communications. 186(1)

Human neutrophil respiratory burst oxidase (NADPH-oxidase) activity can be reconstituted in a cell-free system consisting of plasma membrane, cytosol and an anionic amphiphile [e.g., sodium dodecyl sulfate (SDS) or arachidonate]. Herein, we report re

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d3a7487bd07b26af470b42e79a2743c1
https://pubmed.ncbi.nlm.nih.gov/1321612

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Phorbol myristate acetate (PMA) augments chemoattractant-induced diglyceride generation in human neutrophils but inhibits phosphoinositide hydrolysis. Implications for the mechanism of PMA priming of the respiratory burst

Autor: J D Lambeth, D N Burnham, M Tamura, Shiv Raj Tyagi

Publikováno v: Journal of Biological Chemistry. 263:13191-13198

Pretreatment ("priming") of neutrophils with a non-activating concentration (2 nM) of phorbol myristate acetate (PMA) augments superoxide (O2-) production in response to the chemoattractant formylmethionylleucylphenylalanine (fMLP). We initially exam

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::add264e7bee97f01f5354b10ef8f8138
https://doi.org/10.1016/s0021-9258(18)37690-7

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The superoxide-generating respiratory burst oxidase of human neutrophil plasma membrane. Phosphatidylserine as an effector of the activated enzyme

Autor: Shiv Raj Tyagi, T Tamura, J D Lambeth, M Tamura

Publikováno v: Journal of Biological Chemistry. 263:17621-17626

The superoxide-generating respiratory burst oxidase is an integral membrane enzyme found in the plasma membrane of polymorphonuclear leukocytes (neutrophils). NADPH-dependent superoxide generation is seen in isolated plasma membranes and in their det

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::a066f4cd4be4280a67c3bf27c77a1024
https://doi.org/10.1016/s0021-9258(19)77881-8

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Sphinganine effects on chemoattractant-induced diacylglycerol generation, calcium fluxes, superoxide production, and on cell viability in the human neutrophil. Delivery of sphinganine with bovine serum albumin minimizes cytotoxicity without affecting inhibition of the respiratory burst

Autor: J D Lambeth, Shiv Raj Tyagi, D N Burnham

Publikováno v: Journal of Biological Chemistry. 263:3818-3822

Long chain bases (sphinganine and sphingosine) are potent inhibitors of protein kinase C in an in vitro mixed micelle-reconstituted system (Hannun, Y. A., Loomis, C. R., Merrill, A. H. J., and Bell, R. M. (1986) J. Biol. Chem. 261, 12604-12609) and b

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::268c48c4552fa387553ebefbee1f9f45
https://doi.org/10.1016/s0021-9258(18)68998-7

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On the Biological Occurrence and Regulation of 1-Acyl and 1-O-Alkyl-diradylglycerols in Human Neutrophils

Autor: J D Lambeth, D N Burnham, Shiv Raj Tyagi

Publikováno v: Journal of Biological Chemistry. 264:12977-12982

The occurrence and regulation of 1-ether-linked diradylglycerol in human neutrophils were investigated using a sensitive and practical analytical mass method which distinguishes 1-O-alkyl- (EAG) versus 1-acyl (DAG) diglycerides. After phosphorylation

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::3c951c43d6729c5b7a2bb0afee5315af
https://doi.org/10.1016/s0021-9258(18)51583-0

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Diacylglycerol generation and phosphoinositide turnover in human neutrophils: Effects of particulate versus soluble stimuli

Autor: J. David Lambeth, David J. Uhlinger, D N Burnham, Shiv Raj Tyagi

Publikováno v: Archives of Biochemistry and Biophysics. 269:345-353

Serum-treated, or “opsonized” zymosan (OZ), a particulate material which can be phagocytized by polymorphonuclear leukocytes, activates the superoxide-generating respiratory burst in these cells. The use of dual wavelength spectroscopy in the pre

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::19f075e693b99e8300428e77a7248360
https://doi.org/10.1016/0003-9861(89)90116-1

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