Zobrazeno 1 - 10
of 14
pro vyhledávání: '"Shirou Tsuchida"'
Autor:
Shirou Tsuchida, Takaaki Hirayama, Hayato Nunose, Hinako Suzuki, Ryo Hakota, Tsugumi Shindo, Koji Nakagawa
Publikováno v:
Heliyon, Vol 10, Iss 9, Pp e30465- (2024)
A new UGT1A1*28 detection method combining PCR and high-resolution agarose gel electrophoresis was developed. The viability of this method was demonstrated on 15 healthy adult volunteers. Subjects included 13 wild type homozygotes (86.7 %), 2 heteroz
Externí odkaz:
https://doaj.org/article/0288877df77d404c850deb7afba6ec03
Publikováno v:
Molecular Genetics and Metabolism Reports, Vol 2, Iss C, Pp 41-45 (2015)
d-bifunctional protein (d-BP) deficiency is thought to lead to severe lipid metabolism disorders. To investigate the effect of naturally occurring missense mutations in the hydratase domain in d-BP, we constructed several d-BP hydratase variants and
Externí odkaz:
https://doaj.org/article/55c730ee9e4d4433ba5cb4b8e7edc35a
Publikováno v:
Cellulose. 29:3025-3033
Autor:
Ryusuke Kurosawa, Takumi Kanashiki, Naoya Hamaue, Takuya Ichikawa, Shirou Tsuchida, Shuhei Izumiya, Takashi Aoki
Publikováno v:
FEBS Open Bio
The effect of the addition of sequential C-terminal tryptophan residues on the fluorescence intensity of GFP was investigated. Tandem repeats of six tryptophan residues markedly decreased fluorescence intensity. This phenomenon is likely to occur bec
Autor:
Naoya Hamaue, Ayumu Tenma, Shirou Tsuchida, Takao Kurosawa, Tsuyoshi Murai, Teruki Yoshimura, Takashi Aoki
Publikováno v:
Journal of oleo science. 66(7)
3-oxohexadecanoyl-CoA was synthesized for the study of D-bifunctional protein (EC 4. 2. 1. 107, EC 4. 2. 1. 119, EC 1. 1. 1. n12) and L-bifunctional protein (EC 4. 2. 1. 17, EC 5. 3. 3. 8, EC 1. 1. 1. 35). First, tetradecanal was subjected to the Ref
Publikováno v:
Journal of Oleo Science. 61:443-450
In order to clarify the physiological significance of stereospecificities of peroxisomal multifunctional enzyme (MFE) type 1 (MFE1) and MFE2, we developed a chiral separation analysis for 3-hydroxyacyl-CoA using high performance liquid chromatography
Publikováno v:
Analytical Biochemistry. 378:132-137
Green fluorescent protein (GFP) is very stable for various proteases. Using this property, three protease assay methods designated the disk separation assay for remaining GFP (DSAR), the disk separation assay for liberated GFP (DSAL), and the homogen
Publikováno v:
Biochemical and biophysical research communications. 452(1)
A novel cloning vector that can be used to identify recombinant Escherichia coli colonies by activation of the green fluorescent protein gene (GFP) was constructed. Screening using the vector does not require special reagents. The recombinant plasmid
Autor:
Shirou Tsuchida, Naoya Hamaue, Takashi Aoki, Zhang Xiao, Masakazu Nabeshima, Taemi Yahara, Emi Inoue, Kana Nunome
Publikováno v:
Drug metabolism and pharmacokinetics. 29(1)
Summary: The nucleotide sequences of the proximal promoters of UDP-glucuronosyltransferase (UGT) 1A8 and 1A9 genes are very similar. However, UGT1A8 and 1A9 are mainly expressed in extra-hepatic and hepatic cells, respectively. Using mutants of UGT1A
Autor:
Naoya Hamaue, Takashi Aoki, Kana Nunome, Teruki Yoshimura, Shirou Tsuchida, Takao Kurosawa, Koutarou Kawamoto
Publikováno v:
Journal of oleo science. 60(5)
Enoyl-coenzyme A (CoA) hydratase catalyzes the hydration of trans-2-enoyl-CoA to yield 3-hydroxyacyl-CoA during fatty acid degradation (β-oxidation). Although much research has focused on the stereospecificities of 2-enoyl-CoA hydratases, a direct q