Zobrazeno 1 - 10
of 39
pro vyhledávání: '"Shirley J. Hayes"'
Autor:
Stephen C. Hardies, Karen Lieman, Shirley J. Hayes, Saurav Pathria, Philip Serwer, Mandy Rolando
Publikováno v:
Bacteriophage
We investigate genes of lytic, Bacillus thuringiensis bacteriophage 0305ϕ8-36 that are non-essential for laboratory propagation, but might have a function in the wild. We isolate deletion mutants to identify these genes. The non-permutation of the g
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0c9873e6ce06de11b3181720d30f92f2
https://doi.org/10.4161/bact.19546
https://doi.org/10.4161/bact.19546
Publikováno v:
Journal of Microscopy. 228:309-321
Summary Virus aggregation is analyzed because of its potential use for both classifying viruses and understanding virus ecology and evolution. Virus aggregation is, however, problematic because aggregation causes loss of virions during processing for
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dd8c6493eb0489328aeb52bd9cbbb9d7
https://doi.org/10.1111/j.1365-2818.2007.01855.x
https://doi.org/10.1111/j.1365-2818.2007.01855.x
Autor:
Shehnaz Zaman, Mandy Rolando, Stephen C. Hardies, Shirley J. Hayes, Karen Lieman, Philip Serwer
Publikováno v:
Virology. 329(2):412-424
Isolation and characterization of new environmental bacteriophages are performed for (1) analyzing microbial evolution and ecology and (2) delivering biological therapy. The sampling of environmental bacteriophages appears, however, to be limited by
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::73673ce41a4f1ab59a01a83737ed042a
Autor:
Houyi Wang, Philip Serwer, Shirley J. Hayes, Shijun Huang, Christopher A. Carroll, Susan T. Weintraub
Publikováno v:
ELECTROPHORESIS. 25:779-784
Alexa dye staining of proteins is used for the fluorescence microscopy of single particles that are sometimes multimolecular protein complexes. To characterize the staining, post-staining determination must be made of which protein(s) in a complex ha
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6920e299577a305640f3d7259c0fea4b
https://doi.org/10.1002/elps.200305723
https://doi.org/10.1002/elps.200305723
Publikováno v:
Journal of Microscopy. 213:101-109
To bypass limitations of conventional biochemical analysis, single-particle biochemical analysis is used. To improve single-particle biochemical analysis, procedures are needed to keep nanometre-sized particles in focus while the particles are underg
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c29c0009b1589e52b35f45df6ad58034
https://doi.org/10.1111/j.1365-2818.2004.01284.x
https://doi.org/10.1111/j.1365-2818.2004.01284.x
Autor:
Shirley J. Hayes, Philip Serwer
Publikováno v:
Biophysical Journal. 81(6):3398-3408
To detect partially condensed conformations of a double-stranded DNA molecule, single molecule fluorescence microscopy is performed here. The single DNA molecules are ethidium stained, 670 kilobase pair bacteriophage G genomes that are observed both
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::10a9b708cd3ed09c9a9e9b0970889294
Publikováno v:
Journal of Structural Biology. 135:270-280
To build a foundation for the single-molecule fluorescence microscopy of protein complexes, the present study achieved fluorescence microscopy of single, nucleic acid-free protein capsids of bacteriophage T7. The capsids were stained with Alexa 488 (
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c6ef3aba1bd48bf83a84e85b56186b7c
https://doi.org/10.1006/jsbi.2001.4412
https://doi.org/10.1006/jsbi.2001.4412
Autor:
Olivia Osman, Kelly L. Aubrey, George J. Thomas, Kim E. Reilly, Shirley J. Hayes, Stacy A. Overman, Philip Serwer
Publikováno v:
Biospectroscopy. 4:S47-S56
The structure of the packaged double-stranded DNA genome of bacteriophage T7 was compared to that of unpackaged T7 DNA using digital difference Raman spectroscopy. Spectral data were obtained at 25 degrees C from native T7 virus (100 mg/mL), empty T7
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6d21e4ef21cc7c22f75ee2d5ff9510a1
https://doi.org/10.1002/(sici)1520-6343(1998)4:5+3.0.co
https://doi.org/10.1002/(sici)1520-6343(1998)4:5+
Publikováno v:
Journal of Molecular Biology. 273:658-667
For viruses made of nucleic acid and protein, the structure of the protein outer shell has, in the past, been found to be uniquely determined by the viral genome. However, here, non-denaturing agarose gel electrophoresis of bacteriophage T7 reveals t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b86e57cb73d73f5b6a6e0e064ebcd7d6
https://doi.org/10.1006/jmbi.1997.1353
https://doi.org/10.1006/jmbi.1997.1353
Publikováno v:
Virology. 210:409-420
The morphogenesis of bacteriophage T7 includes assembly of a procapsid that subsequently both packages DNA and changes in structure. The DNA packaged by T7 is concatemeric and is cleaved to mature length during packaging. In the present study, packag
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::104a466e1a7afde6c6eef98a6005b073
https://doi.org/10.1006/viro.1995.1357
https://doi.org/10.1006/viro.1995.1357