Zobrazeno 1 - 10
of 31
pro vyhledávání: '"Shinya Kurata"'
Autor:
Shinya Kurata, Yasufumi Matsumura, Yasushi Enomoto, Hiroshi Habe, Tetsuya Nakazato, Hidenori Tani, Masahiro Yamaguchi
Publikováno v:
Analytical Biochemistry. 617:114114
We developed a novel nanocomposite bead system for detection by the naked eye of specific DNA sequences amplified by the polymerase chain reaction (PCR). The DNA probes, which were complementary to the target DNA, are conjugated with the nanocomposit
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::08a7b0b2e1b74a7167616a11f3f1939f
https://doi.org/10.1016/j.ab.2021.114114
https://doi.org/10.1016/j.ab.2021.114114
Publikováno v:
Nucleic Acids Research
Ribosomes, after one round of translation, must be recycled so that the next round of translation can occur. Complete disassembly of post-termination ribosomal complex (PoTC) in yeast for the recycling consists of three reactions: release of tRNA, re
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c81dd2e56ec320930ea7e023af2ca8c1
https://doi.org/10.1093/nar/gks958
https://doi.org/10.1093/nar/gks958
Autor:
Shinya Kurata, Satoshi Tsuneda, Toyokazu Yokomaku, Takeshi Terahara, Shigeaki Harayama, Kazutaka Yamada
Publikováno v:
Enzyme and Microbial Technology. 47:17-23
Putative esterase genes were isolated from environmental DNA by using pre-amplified inverse PCR. The sequence analysis of the isolated genes showed 32–80% amino acid sequence identities to known esterases/lipases in public databases. The isolated g
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6eb18fabaa881eda9e37a7e13eef7e89
https://doi.org/10.1016/j.enzmictec.2010.04.005
https://doi.org/10.1016/j.enzmictec.2010.04.005
Autor:
Ryo Miyata, Yuji Sekiguchi, Ken Adachi, Hidenori Tani, Shinya Kurata, Naohiro Noda, Kazunori Nakamura, Satoshi Tsuneda
Publikováno v:
Molecular and Cellular Probes. 24:131-137
Dehalococcoides spp. are responsible for the reductive dehalogenation of environmental contaminants and are candidates for engineered bioremediation. The development of a sensitive, reliable, and rapid method for the quantification of Dehalococcoides
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::75d689fbcbdfcb4de7f0269affb1bfea
https://doi.org/10.1016/j.mcp.2009.11.005
https://doi.org/10.1016/j.mcp.2009.11.005
Publikováno v:
Proceedings of the National Academy of Sciences. 107:10854-10859
After each round of protein biosynthesis, the posttermination complex (PoTC) consisting of a ribosome, mRNA, and tRNA must be disassembled into its components for a new round of translation. Here, we show that a Saccharomyces cerevisiae model PoTC wa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c0c815a3ef956ca6f7afe3365fa33c27
https://doi.org/10.1073/pnas.1006247107
https://doi.org/10.1073/pnas.1006247107
Autor:
Satoshi Tsuneda, Naohiro Noda, Kazunori Nakamura, Yuji Sekiguchi, Shinya Kurata, Ryo Miyata, Kouhei Ichikawa, Hidenori Tani, Soji Morishita
Publikováno v:
Analytical Chemistry. 81:5678-5685
We have developed a flexible, specific, and cost-effective real-time polymerase chain reaction (PCR) method. In this technique, a quenching probe (QProbe) and a nonfluorescent 3'-tailed probe are used. The QProbe is a singly labeled oligonucleotide b
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::04344505fca0e70d993748e00b9dab3b
https://doi.org/10.1021/ac900414u
https://doi.org/10.1021/ac900414u
Autor:
Soji Morishita, Yuji Sekiguchi, Shinya Kurata, Satoshi Tsuneda, Naohiro Noda, Kazunori Nakamura, Hidenori Tani
Publikováno v:
Clinical Biochemistry. 42:515-520
Objectives This study developed a novel MRD monitoring method targeting Wilms' tumor gene (WT1) mRNA using reverse transcription loop-mediated isothermal amplification (RT-LAMP). Design and methods A primer set for the assay was designed on the basis
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::916fb8732a33e0d53a9e07e2f44193c7
https://doi.org/10.1016/j.clinbiochem.2009.01.013
https://doi.org/10.1016/j.clinbiochem.2009.01.013
Autor:
A. Manome, Shinya Kurata, T. Yokomaku, Yoichi Kamagata, O. Koyama, Takahiro Kanagawa, A. Kageyama, Hideyuki Tamaki, Masahiro Tagawa
Publikováno v:
Plant Pathology. 57:887-896
Common scab of potato tubers caused by pathogenic Streptomyces spp. is a cause of serious economic loss worldwide. For the rapid and accurate quantification of pathogenic Streptomyces spp. residing in soil, a new competitive real-time PCR method usin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b64500fca1ca2ccad8a33d2710aa4e6b
https://doi.org/10.1111/j.1365-3059.2008.01881.x
https://doi.org/10.1111/j.1365-3059.2008.01881.x
Autor:
Takashi Ohtsuki, Kimitsuna Watanabe, Kazuyuki Takai, Takeshi Wada, Venki Ramakrishnan, Shinya Kurata, Albert Weixlbaumer, Tomomi Shimazaki, Tsutomu Suzuki, Yohei Kirino
Publikováno v:
Journal of Biological Chemistry. 283:18801-18811
Post-transcriptional modifications at the first (wobble) position of the tRNA anticodon participate in precise decoding of the genetic code. To decode codons that end in a purine (R) (i.e. NNR), tRNAs frequently utilize 5-methyluridine derivatives (x
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8396bcb39a453bf59f6ae5054517f333
https://doi.org/10.1074/jbc.m800233200
https://doi.org/10.1074/jbc.m800233200
Autor:
Toyokazu Yokomaku, Shigeaki Harayama, Shinya Kurata, Takeshi Terahara, Kazutaka Yamada, Satoshi Tsuneda
Publikováno v:
Environmental Microbiology
Summary We had been unsuccessful to amplify desired nucleotide sequences from various environmental DNA samples by using the inverse polymerase chain reaction (IPCR) technique, most probably because the copy numbers of target DNA sequences had been q
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e8903c8d84c6ccdefbcf592f49ef3ffc
https://doi.org/10.1111/j.1462-2920.2007.01518.x
https://doi.org/10.1111/j.1462-2920.2007.01518.x