Zobrazeno 1 - 10
of 11
pro vyhledávání: '"Shintaro Iwatani"'
Autor:
Yasuhiro Takahashi, Toshiharu Hase, Shintaro Iwatani, Kiyoshi Matsuno, Satoshi Okutani, Takayoshi Iwai
Publikováno v:
Journal of Bioscience and Bioengineering. 120:287-293
During amino-acid crystal fermentation, mechanical stress on bacterial cells caused by crystal collision often impacts negatively on bacterial growth and amino-acid production. When Escherichia coli cells were cultivated under mechanical stress of po
Autor:
Takayoshi Iwai, Shintaro Iwatani, Tsuyoshi Osumi, Nobuharu Tsujimoto, Satoshi Okutani, Kiyoshi Matsuno, Kazuya Kondo
Publikováno v:
Journal of Bioscience and Bioengineering. 113:487-490
We investigated the mechanical damage to the Escherichia coli cell caused by polyvinyl chloride particles as a model of amino-acid crystal fermentation. Our results indicated that the glucose-consumption rate and the intracellular ATP concentration t
Autor:
Yosuke Nishio, Kazuhiko Matsui, Akira Imaizumi, Shintaro Iwatani, Naoko Kageyama, Yoshihiro Usuda, Daigo Iwahata, Kazutaka Shimbo, Hiroshi Miyano, Stephen J. Van Dien
Publikováno v:
Journal of Biotechnology. 147:17-30
Our aim is to construct a practical dynamic-simulation system that can model the metabolic and regulatory processes involved in the production of primary metabolites, such as amino acids. We have simulated the production of glutamate by transient bat
Publikováno v:
Biotechnology Letters. 30:791-799
Metabolic flux analysis (MFA) has become a fundamental tool of metabolic engineering to elucidate the metabolic state of the cell and has been applied to various biotechnological processes. In recent years, considerable technical advances have been m
Autor:
Kazuyuki Shimizu, Kazutaka Shimbo, Kazuo Hirayama, Yoshihiro Usuda, Kazuyuki Kubota, Stephen J. Van Dien, Daigo Iwahata, Shintaro Iwatani, Naoko Kageyama, Kazuhiko Matsui, Hiroshi Miyano
Publikováno v:
Journal of Biotechnology. 128:93-111
Metabolic flux analysis using 13C-labeled substrates is a well-developed method for investigating cellular behavior in steady-state culture condition. To extend its application, in particular to typical industrial conditions, such as batch and fed-ba
Autor:
Shintaro Iwatani, Motomu Nishioka, Yoshihiro Ojima, Masahito Taya, Nobuharu Tsujimoto, Mizuho Komaki
Publikováno v:
Biotechnology letters. 31(4)
A stress-responsive gene, yggG, was introduced into an L-phenylalanine producer, Escherichia coli AJ12741. In shake-flask culture, the yggG-containing recombinant strain (named AJ12741/pHYGG) produced 6.4 g L-phenylalanine l(-1) at the end of culture
Publikováno v:
Journal of bioscience and bioengineering. 102(1)
This work demonstrates a novel computational approach combining flux balance modeling with statistical methods to identify correlations among fluxes in a metabolic network, providing insight as to how the fluxes should be redirected to achieve maximu
Autor:
Mikhail S Shupletsov, Lyubov I Golubeva, Svetlana S Rubina, Dmitry A Podvyaznikov, Shintaro Iwatani, Sergey V Mashko
Publikováno v:
Microbial Cell Factories. 13:152
Publikováno v:
Biochemistry. 38(32)
To probe the structural changes within kinesin molecules, we made the mutants of motor domains of two-headed kinesin (4-411 aa) in which either all the five cysteines or all except Cys45 were mutated. A residual cysteine (Cys45) of the kinesin mutant
Autor:
Yoshihiro Ojima, Masahito Taya, Shintaro Iwatani, Mizuho Komaki, Nobuharu Tsujimoto, Motomu Nishioka
Publikováno v:
Journal of Bioscience and Bioengineering. 108:S114