High-throughput analysis system of interaction kinetics for data-driven antibody design

Autor: Ryo Matsunaga, Kan Ujiie, Mayuko Inagaki, Jorge Fernández Pérez, Yoshiki Yasuda, Shinya Mimasu, Shinji Soga, Kouhei Tsumoto

Publikováno v: Scientific Reports, Vol 13, Iss 1, Pp 1-9 (2023)

Abstract Surface plasmon resonance (SPR) is widely used for antigen–antibody interaction kinetics analysis. However, it has not been used in the screening phase because of the low throughput of measurement and analysis. Herein, we proposed a high-t

Externí odkaz: https://doaj.org/article/bc2db471440047b7834aef6961058f18

Generation and Characterization of a Novel Anti-Rat TLR4/MD2 Antibody with Potent Neutralizing Activity In Vivo

Autor: Masahito Sato, Jotaro Suzuki, Satoshi Takeuchi, Shinji Soga, Koji Aoyama

Publikováno v: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy. 39:217-221

Toll-like receptor 4 (TLR4) plays a critical role in the innate immune system and is involved in the pathogenesis of multiple diseases. Here, we report the antagonistic and ratized antibody, 52-1H4 e2 (e2), which completely inhibited lipopolysacchari

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::44799d2de3f8a679e1e71fa8b8cfee65
https://doi.org/10.1089/mab.2020.0020

Computational design, construction, and characterization of a set of specificity determining residues in protein-protein interactions

Autor: Nozomi Izako, Kenji Mizuguchi, Chioko Nagao, Shigeki Kawabata, Samia Haseeb Khan, Shinji Soga, Hiroki Shirai

Publikováno v: Proteins: Structure, Function, and Bioinformatics. 80:2426-2436

Proteins interact with different partners to perform different functions and it is important to elucidate the determinants of partner specificity in protein complex formation. Although methods for detecting specificity determining positions have been

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bab0de37ca43b4b817e2528fbe7c213a
https://doi.org/10.1002/prot.24127

Identification of the Druggable Concavity in Homology Models Using the PLB Index

Autor: Masato Kobori, Hiroki Shirai, Shinji Soga, Noriaki Hirayama

Publikováno v: Journal of Chemical Information and Modeling. 47:2287-2292

Identification of the druggable concavity, in which drug-like molecules are highly inclined to bind, is an important step in structure-based drug design. We previously proposed an index named PLB (propensity for ligand binding), which is based on the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c9f1dcedd67109507fa45b4522f1acf0
https://doi.org/10.1021/ci7002363

Antibody informatics for drug discovery

Autor: Kazuyoshi Ikeda, Kazuhisa Tsunoyama, Dominic Clark, John P. Overington, Hiroki Shirai, Paolo Marcatili, Catherine Prades, Randi Vita, Jianqing Xu, Hirayama Kazunori, Marie-Paule Lefranc, Shinji Soga, Bojana Popovic

Publikováno v: BBA-Biochimica et Biophysica Acta
BBA-Biochimica et Biophysica Acta, Elsevier, 2014, 1844 (11), pp.2002-2015. ⟨10.1016/j.bbapap.2014.07.006⟩

More and more antibody therapeutics are being approved every year, mainly due to their high efficacy and antigen selectivity. However, it is still difficult to identify the antigen, and thereby the function, of an antibody if no other information is

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b4939ebc5de3b756fa9dc4c8c5e65f3c
https://hal.archives-ouvertes.fr/hal-01088585