Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Shinichi Fukuzono"'
Publikováno v:
Analytica Chimica Acta. 564:53-58
Protein microarray technology is a powerful, popular tool for the high-throughput analysis of protein interactions. One important use for protein microarray technology is protein quantification by immunoassay, which was originally based on enzyme lin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ac047c117e86255eb4d2408850ab1521
https://doi.org/10.1016/j.aca.2005.08.033
https://doi.org/10.1016/j.aca.2005.08.033
Autor:
Takuya Matsui, Katsuhiro Kanda, Tokiko Yoshimura-Suzuki, Moon Hi Han, Yukie Sasakura, Toru Shimizu, Shinichi Fukuzono
Publikováno v:
Analytical Chemistry. 76:6521-6527
A highly sensitive microarray system for detecting protein-protein interactions has been developed. This method was successfully applied to analyze the interactions of heme-regulated phosphodiesterase from Escherichia coli (Ec DOS). To immobilize (Hi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d3400bf4dc000d5e0a90b04e8942832c
https://doi.org/10.1021/ac048832t
https://doi.org/10.1021/ac048832t
Autor:
Mariko Taylor, Satoshi Ozawa, Tsuyoshi Sonehara, Akira Ichikawa, Yuji Miyahara, Takashi Irie, Noriko Fukayama, Kokichi Sugano, Shinichi Fukuzono
Publikováno v:
Analytical Chemistry. 76:6122-6129
Since the successful completion of the Human Genome Project, increasing concern is being directed toward the polymorphic aspect of the genome and its clinical relevance. A form of single-strand DNA-conformation polymorphism analysis (SSCP) employing
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aba7e92290470c1b7c4d90d1463252c5
https://doi.org/10.1021/ac049385k
https://doi.org/10.1021/ac049385k
Autor:
Shinichi Fukuzono, Akiko Hisada, Katsuhiko Mikoshiba, Tomoko Takeshita, Norio Shimizu, Takeshi Sakamoto
Publikováno v:
Biochemical and Biophysical Research Communications. 249:66-72
Myelin proteolipid protein (PLP) is a major integral membrane protein of central nervous system myelin and is considered to play a significant role in myelination. PLP has a four-transmembrane structure, judging from the hydropathy profile. In additi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d8c0d41dbff3a341a72949e0b91548bf
https://doi.org/10.1006/bbrc.1998.8926
https://doi.org/10.1006/bbrc.1998.8926
Autor:
Norio Shimizu, Tadashi Ishibashi, Shinichi Fukuzono, Tomoko Takeshita, Kyungmoon Park, Kiyoshi Fujimori
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 60:971-974
E. coli cells harboring an expression plasmid with a brain-derived neurotrophic factor (BDNF) gene obtained from rat BDNF cDNA were sonicated and centrifuged to obtain a precipitate containing BDNF. Ten different proteins of BDNF were purified from t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5218677cbd9f46b378f6ea0edb09f815
https://doi.org/10.1271/bbb.60.971
https://doi.org/10.1271/bbb.60.971
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 56:1985-1990
A gene coding for human nerve growth factor (hNGF) was constructed for expression under control of the trp promoter in E. coli. The plasmid pTRSNGF contained a synthetic hNGF gene fused, in frame, to the region encoding the beta-lactamase signal pept
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::23432d76b882f1b50bd1f8afbaaec8ee
https://doi.org/10.1271/bbb.56.1985
https://doi.org/10.1271/bbb.56.1985
Publikováno v:
Sensors and Actuators B: Chemical. 66:19-21
We developed a receptor-mediated biological sensor for real time detection of histamine derived from granules of mast cell and basophils. A human histamine receptor gene was cloned by polymerase chain reaction and its mRNA was injected into Xenopus o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::49134da94e7d22181c405cfdff7d81e1
https://doi.org/10.1016/s0925-4005(99)00472-4
https://doi.org/10.1016/s0925-4005(99)00472-4
Autor:
Tokiko Yoshimura-Suzuki, Takuya Matsui, Toru Shimizu, Katsuhiro Kanda, Yukie Sasakura, Shinichi Fukuzono
Publikováno v:
Biochemistry. 44(28)
Ec DOS, a heme-regulated phosphodiesterase from Escherichia coli, is composed of an N-terminal heme-bound PAS domain and a C-terminal phosphodiesterase domain. The heme redox state in the PAS domain regulates Ec DOS phosphodiesterase activity. Intere
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8288cb675640572efa2165daefbd00ad
https://pubmed.ncbi.nlm.nih.gov/16008345
https://pubmed.ncbi.nlm.nih.gov/16008345
Publikováno v:
Bioscience, biotechnology, and biochemistry. 59(9)
A gene coding for a brain-derived neurotrophic factor (BDNF) obtained from rat BDNF cDNA was used to construct an expression plasmid, pTRSBDNF. It contained a BDNF gene that was fused, in frame, to a region encoding the beta-lactamase signal peptide.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5ec99a7bf4d4d63c579f3b5bcf5af6cb
https://pubmed.ncbi.nlm.nih.gov/8520114
https://pubmed.ncbi.nlm.nih.gov/8520114
Autor:
Shinichi Fukuzono, Kiyoshi Fujimori, Yoshinori Harada, Norio Shimizu, Yoshio Yamazaki, Kenichi Gotoh
Publikováno v:
Biotechnology and bioengineering. 38(1)
Fed-batch cultures of recombinant E. coli HB101 harboring expression plasmid pTRLBT1 or pTREBT1, with acetate concentration monitoring, are investigated to obtain high cell density and large amounts of human epidermal growth factor (hEGF). The expres
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7183c76e95aa98a42b6ef7355dffc208
https://pubmed.ncbi.nlm.nih.gov/18600696
https://pubmed.ncbi.nlm.nih.gov/18600696