Zobrazeno 1 - 10
of 48
pro vyhledávání: '"Shin-ichi Sakasegawa"'
Autor:
Kazu Hatanaka, Yasushi Shirahase, Toshiyuki Yoshida, Mari Kono, Naoki Toya, Shin-Ichi Sakasegawa, Kenji Konishi, Tadashi Yamamoto, Kuniyasu Ochiai, Shogo Takashiba
Publikováno v:
PLoS ONE, Vol 17, Iss 7, p e0268671 (2022)
Periodontal disease is a chronic inflammatory condition caused by periodontal pathogens in the gingival sulcus. Short-chain fatty acids (SCFAs) produced by causal bacteria are closely related to the onset and progression of periodontal disease and ha
Externí odkaz:
https://doaj.org/article/b8053b94526a451e8ad19ad0ed6a9eda
Autor:
Hiroaki Hamana, Yoshiaki Yasutake, Miyuki Kato-Murayama, Toshiaki Hosaka, Mikako Shirouzu, Shin-ichi Sakasegawa, Daisuke Sugimori, Kazutaka Murayama
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 87:74-81
Lysoplasmalogen-specific phospholipase D (LyPls-PLD) hydrolyzes choline lysoplasmalogen to choline and 1-(1-alkenyl)-sn-glycero-3-phosphate. Mutation of F211 to leucine altered its substrate specificity from lysoplasmalogen to 1-O-hexadecyl-2-hydroxy
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::347dcc6be424bba294a018b0ae49b86d
https://doi.org/10.1093/bbb/zbac169
https://doi.org/10.1093/bbb/zbac169
Autor:
Kenji Konishi, Yoshiaki Yasutake, Shuji Muramatsu, Satomi Murata, Keitaro Yoshida, Koji Ishiya, Sachiyo Aburatani, Shin-ichi Sakasegawa, Tomohiro Tamura
Publikováno v:
Applied microbiology and biotechnology. 106(24)
Burkholderia stabilis strain FERMP-21014 secretes cholesterol esterase (BsChe), which is used in clinical settings to determine serum cholesterol levels. Previously, we constructed an expression plasmid with an endogenous constitutive promoter to ena
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e4ff3c7c6dbe95ace254f571a59b2273
https://pubmed.ncbi.nlm.nih.gov/36399168
https://pubmed.ncbi.nlm.nih.gov/36399168
Autor:
Keitaro Yoshida, Tomohiro Tamura, Shin-ichi Sakasegawa, Yoshiaki Yasutake, Shuji Muramatsu, Sachiyo Aburatani, Kenji Konishi
Publikováno v:
International Journal of Biological Macromolecules. 167:578-586
Cholesterol esterase (Che) from Burkholderia stabilis (BsChe) is a homolog of well-characterized and industrially relevant bacterial triacylglycerol lipases (Lips). BsChe is a rare bacterial Lip enzyme that exhibits practical Che activity and is curr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::47b25e7bf9e3e1af2ce46d77ce37b753
https://doi.org/10.1016/j.ijbiomac.2020.11.184
https://doi.org/10.1016/j.ijbiomac.2020.11.184
Publikováno v:
FEBS lettersReferences. 596(8)
Hypoxanthine-guanine phosphoribosyltransferase (HGPRT) is a key enzyme in the purine salvage pathway. Here, the reverse reaction of HGPRT from the thermophilic bacterium Hungateiclostridium thermocellum was studied in the presence of IMP and pyrophos
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::23842fdc22cc638f5267dc3f674fbdd2
https://pubmed.ncbi.nlm.nih.gov/35114018
https://pubmed.ncbi.nlm.nih.gov/35114018
Autor:
Hiroaki Hamana, Yoshiaki Yasutake, Miyuki Kato-Murayama, Toshiaki Hosaka, Mikako Shirouzu, Shin-ichi Sakasegawa, Daisuke Sugimori, Kazutaka Murayama
Publikováno v:
Bioscience, Biotechnology & Biochemistry; Jan2023, Vol. 87 Issue 1, p74-81, 8p
Autor:
Sachiyo Aburatani, Shuji Muramatsu, Shin-ichi Sakasegawa, Yoshiaki Yasutake, Keitaro Yoshida, Tomohiro Tamura, Satomi Murata, Arturo Magana-Mora, Kenji Konishi, Toshitaka Kumagai, Adrien Rougny
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 83:1974-1984
Burkholderia stabilis FERMP-21014 produces highly active cholesterol esterase in the presence of fatty acids. To develop an overexpression system for cholesterol esterase production, we carried out RNA sequencing analyses to screen strongly active pr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::032a407b781769a3be2ee61987194c99
https://doi.org/10.1080/09168451.2019.1630256
https://doi.org/10.1080/09168451.2019.1630256
Autor:
Shin-ichi Sakasegawa, Shigeru Ueda
Publikováno v:
Analytical Biochemistry. 570:27-31
Previously, we developed a kinase cycling method using creatine kinase and pyruvate kinase (RMPK) both from rabbit muscle in the presence of an excess amount of ATP and IDP for the quantitative determination of substrate. To our surprise, the RMPK cy
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c829dda39dc620cae6246cf079a656bb
https://doi.org/10.1016/j.ab.2019.02.005
https://doi.org/10.1016/j.ab.2019.02.005
Autor:
Shigeru Ueda, Shin-ichi Sakasegawa
Publikováno v:
Bioscience, biotechnology, and biochemistry. 85(5)
For assaying serum creatinine, the enzymatic method is regarded as accurate. However, a reliable measurement of low levels is problematic. We have developed a new method that utilizes an enzymatic cycling reaction involving creatine kinase (CK) in th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1a425b25d75d580e7b96bd51c5765a32
https://pubmed.ncbi.nlm.nih.gov/33629709
https://pubmed.ncbi.nlm.nih.gov/33629709
Autor:
Shin-ichi Sakasegawa, Susumu Osawa, Atsushi Isobe, Tsukuru Umemura, Miki Yajima, Masanori Seimiya, Yuki Iwabuchi, Yoshitaka Yamaguchi
Publikováno v:
Clinica chimica acta; international journal of clinical chemistry. 511
Background and aims Polymerase chain reaction-based techniques require expensive equipment for fluorescence detection of the products. However, the measurement of inorganic pyrophosphate (PPi) released during DNA synthesis can be used to quantify tar
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8297e438e235fca0de68f6eea5fabca7
https://pubmed.ncbi.nlm.nih.gov/33096031
https://pubmed.ncbi.nlm.nih.gov/33096031