Zobrazeno 1 - 10
of 19
pro vyhledávání: '"Shin Isogai"'
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-13 (2020)
Signal transmission and regulation of G-protein-coupled receptors (GPCRs) by ligands occurs via modulation of complex conformational equilibria. Here authors quantify these equilibria and their dynamics in the β1-adrenergic receptor in its apo form
Externí odkaz:
https://doaj.org/article/8bb35a77f6744d2dbe67f65cb91afeaf
Publikováno v:
Current topics in behavioral neurosciences. 52
GABA
Publikováno v:
Behavioral Neurobiology of GABAB Receptor Function ISBN: 9783030913342
GABAB receptors (GBRs), the G protein-coupled receptors for the inhibitory neurotransmitter γ-aminobutyric acid (GABA), activate Go/i-type G proteins that regulate adenylyl cyclase, Ca2+ channels, and K+ channels. GBR signaling to enzymes and ion ch
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9a26aee23d68b880bb0b2faa96d3c1f5
https://doi.org/10.1007/7854_2020_147
https://doi.org/10.1007/7854_2020_147
Autor:
Alvar D. Gossert, Shin Isogai, Leonildo Delgado, Anne Grahl, Christian Opitz, Stephan Grzesiek, Bastian Franke
Publikováno v:
Journal of Biomolecular NMR. 71:173-184
Baculovirus-infected insect cells have become a powerful tool to express recombinant proteins for structural and functional studies by NMR spectroscopy. This article provides an introduction into the insect cell/baculovirus expression system and its
Versatile modules enable automated multi-column purifications on the ÄKTA pure chromatography system
Publikováno v:
Journal of chromatography. A. 1618
Protein purification processes in basic research using AKTA™ liquid chromatography systems are often limited to single sample injections and simple one-column purifications. Because many target proteins in structural biology require complex purific
Autor:
Stephan Grzesiek, Franziska M. Heydenreich, Shin Isogai, Christian Opitz, Ching-Ju Tsai, Xavier Deupi, Dmitry B. Veprintsev, Florian Brueckner, Gebhard F. X. Schertler
Publikováno v:
Nature
G protein-coupled receptors (GPCRs) are physiologically important transmembrane signalling proteins that trigger intracellular responses upon binding of extracellular ligands. Despite recent breakthroughs in GPCR crystallography, the details of ligan
Publikováno v:
Separation and Purification Technology. 240:116556
Recent advances in instrumentation of fast protein liquid chromatography and the availability of various pre-packed columns helped to standardize protein purification protocols. However, purification processes are often plagued by inefficient manual
Autor:
Masahiro Shirakawa, Hidehito Tochio, Motoko Unoki, Mamoru Sato, Kyohei Arita, Kazuya Sugita, Ryuji Hamamoto, Shin Isogai, Mariko Ariyoshi, Takashi Oda, Naotaka Sekiyama, Keiko Kuwata
Publikováno v:
Proceedings of the National Academy of Sciences. 109:12950-12955
Multiple covalent modifications on a histone tail are often recognized by linked histone reader modules. UHRF1 [ubiquitin-like, containing plant homeodomain (PHD) and really interesting new gene (RING) finger domains 1], an essential factor for maint
Autor:
Hidehito Tochio, Masahiro Shirakawa, Megumi Tsuchiya, Hiroaki Taniguchi, Yasushi Hiraoka, Tokuko Haraguchi, Hidesato Ogawa, Ken Ichirou Morohashi, Shin Isogai
Publikováno v:
Scientific Reports
Transcriptional coregulators contribute to several processes involving nuclear receptor transcriptional regulation. The transcriptional coregulator androgen receptor-interacting protein 4 (ARIP4) interacts with nuclear receptors and regulates their t
Publikováno v:
Journal of biomolecular NMR
Heterologous expression of proteins in insect cells is frequently used for crystallographic structural studies due to the high yields even for challenging proteins requiring the eukaryotic protein processing capabilities of the host. However for NMR