Zobrazeno 1 - 4
of 4
pro vyhledávání: '"Shiho Onodera"'
Publikováno v:
Electrophoresis Letters. 61:84-86
Autor:
Shiho Onodera, Izurni Kumagai, Ai Kurihara, Ryutaro Asano, Tomoyuki Yasukawa, Tomokazu Matsue, Hitoshi Shiku, Kuniaki Nagamine
Publikováno v:
Biotechnology and Bioengineering. 96:1008-1013
A microbial array chip with collagen gel spots entrapping living Escherichia coli (E. coli) DH5α was applied for the screening of recombinant protein solubilities. The α-fragment of β-galactosidase (βGal) was fused to the target protein, namely,
Autor:
Tsunenori Nozawa, Masayuki Kobayashi, Zheng-Yu Wang, Shiho Onodera, Hiroaki Suzuki, Yuichiro Shimada
Publikováno v:
Photochemistry and Photobiology.
Heterologous expression of the PufX membrane protein from purple photosynthetic bacterium Rhodobacter sphaeroides was attempted by using Escherichia (E.) coli cells. The PufX was overexpressed as a recombinant protein with a histidine tag added to th
Autor:
Tomoyuki Yasukawa, Hitoshi Shiku, Kuniaki Nagamine, Yu Suke Torisawa, Shiho Onodera, Tomokazu Matsue
Publikováno v:
Analytical chemistry. 77(13)
On-chip transformation of Escherichia coli cells was accomplished for the first time using a microbial array chip. The continuous E. coli transformation procedures were performed on a chip in which the microcompartment was composed of PDMS microfluid