Zobrazeno 1 - 10
of 56
pro vyhledávání: '"Shigeyuki Ichihara"'
Publikováno v:
Science of The Total Environment. 381:233-242
Dechlorination of PCP has been observed previously under anaerobic condition in paddy soil. However, there is poor information about the dechlorination pathway of PCP and the microbial community associated with the PCP dechlorination in paddy soil. I
Autor:
Akira Goto, Hitoshi Mori, Ryo Narita, Shigeyuki Ichihara, Hiromi Imai, Hironobu Yamashita, Yasuo Kitagawa
Publikováno v:
FEBS Letters. 576:127-132
Heparin-column chromatography and elastase-digestion of medium from hemocyte Kc167 gave Drosophila laminin alpha3/5betagamma trimer, alpha3/5LG2-3 and alpha3/5LG4-5 modules with eluting NaCl concentrations of 450, 280 and 450 mM, respectively. Kc167
Autor:
Fuminobu Yoshimura, Yuichi Mukai, Hiroshi Nakamura, Yukitaka Murakami, Shigeyuki Ichihara, Masashi Imai
Publikováno v:
FEMS Microbiology Letters. 230:159-165
We examined the effects of various culture environments on major outer membrane proteins from Porphyromonas gingivalis ATCC 33277. Major outer membrane protein patterns on gel electrophoresis showed little difference over the culturable range of osmo
Autor:
Hidetoshi Inagaki, Shigeyuki Ichihara, Sumio Maeda, Takao Saito, Mayuko Takada, Kimiko Tanaka
Publikováno v:
Cell Biology International. 26:635-640
We have isolated mouse DLG6 (mDLG6) cDNA clones by RT-PCR and then by using the RT-PCR products to screen a mouse brain cDNA library. The deduced amino acid sequence of mDLG6 shows 79.2% and 82.7% overall identity to human (hDLG6) and rat DLG6 (rDLG6
Publikováno v:
Biochemical Journal. 360:167-172
Drosophila laminin αβγ trimer assembly in Kc167 cells was perturbed by chain-specific RNA interference (RNAi). The intracellular pool of α and γ chains remained unchanged under β-chain RNAi by lipofection of double-stranded RNA encoding a β-ch
Autor:
Hiroshi Mori, Shin-ichiro Yokoyama, Shuichi Shiraishi, Nobuko Ohishi, Tohru Suzuki, Shigeyuki Ichihara, Kunio Yagi, Saori Itoh
Publikováno v:
Journal of Clinical Biochemistry and Nutrition. 30:33-42
We constructed isogenic Shiga (-like) toxin (Stx-1 and/or Stx-2) gene-deletion mutants of enterohemorrhagic Escherichia coli (EHEC) GPU96MM (O157:H7). A vector with temperature-sensitive replication origin was used for the construction. The parts of
Publikováno v:
Journal of Biochemistry. 119:703-710
To elucidate the role of a flexible loop (residues 142-149) in the stability and function of Escherichia coli dihydrofolate reductase, alanine-145 in this loop was substituted by site-directed mutagenesis with ten amino acids (Glu, Phe, Gly, His, Ile
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 68:746-748
The phenylacetic acid (PhAc) degradation pathway becomes an interesting model for the catabolism of aromatic compounds. To determine the molecular basis for this environmentally important process, we did a phylogenic analysis based on the PhAc CoA li
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 59:256-261
Escherichia coli excretes acetate during aerobic growth in a rich medium, L-broth containing 0.4% glucose, and growth ceases before depletion of glucose because of the decrease in pH caused by the accumulation of acetate. The addition of sodium phosp
Publikováno v:
The Journal of Biochemistry. 116:916-922
The pta gene encoding phosphotransacetylase was cloned on a high copy plasmid with or without the ackA gene encoding acetate kinase in Escherichia coli. The acetate kinase and phosphotransacetylase were overproduced in cells harboring the plasmid pos