Zobrazeno 1 - 10
of 22
pro vyhledávání: '"Shigeru Tamatsukuri"'
Autor:
Toyoki Moribe, Shigeru Tamatsukuri, Norio Iizuka, N Fujita, Isao Sakaida, T Miura, Kazuhiko Sakamoto, Hideo Ishitsuka, Kouichi Uchida, Yoshihiro Tokuhisa, Takao Tamesa, Shuji Terai, Masaaki Oka
Publikováno v:
British Journal of Cancer
In a previous study, we showed that levels of cell-free DNA (cfDNA) were significantly higher in sera of patients with hepatocellular carcinoma (HCC) associated with hepatitis C virus (HCV) than in sera of non-HCC patients with HCV. To confirm this f
Autor:
Junko Kumagai, Keiko Katayama, Yutaka Komiya, Masaaki Mizui, Tetsushi Tomoguri, Junko Tanaka, Shinya Kishimoto, Yuzo Miyakawa, Shigeru Tamatsukuri, Hisao Yugi, Retsuji Yamanaka, Hiroshi Yoshizawa
Publikováno v:
Intervirology. 47:57-64
Objective: To determine the copy number of hepatitis C virus (HCV) RNA, determined by nucleic acid amplification test (NAT) for screening blood units in Japan, that can transmit infection to chimpanzees. Methods: Fresh-frozen plasma with markers of H
Autor:
L Cheng, Chaka Impraim, Toru Hirose, Y Sakakura, T Ramankutty, M Sasaki, C Wong, David Clarke, A Rangachari, H Yawata, Q Meng, Shigeru Tamatsukuri, E Fiss
Publikováno v:
Journal of Clinical Microbiology. 39:2937-2945
We have developed an automated multiplex system for simultaneously screening hepatitis B virus (HBV), hepatitis C virus (HCV), and human immunodeficiency virus type 1 (HIV-1) in blood donations. The assay, designated AMPLINAT MPX HBV/HCV/HIV-1 Test (
Publikováno v:
Journal of the Japanese Association for Infectious Diseases. 70:479-484
A novel method for the extraction of HCV-RNA by using DNA probe coated-latex microparticle (AmpliTex) was developed. This high density particle, with a large surface area, could be dispersed uniformly in a guanidium lysate of the serum. The DNA probe
Autor:
Takako Hayashi, Hiroyuki Tano, Mitsunobu Imai, Shigeru Tamatsukuri, Masaaki Kitajima, Yasuhiro Furuichi, Kejun Fan
Publikováno v:
Journal of the Japanese Association for Infectious Diseases. 69:957-962
PCR mediated detection of HIV-DNA has been widely used. However, compared with traditional immunological diagnoses, the extraction of DNA is a laborious and time consuming step. We have developed a procedure for the efficient isolation and concentrat
Autor:
Chikara Miyamoto, Yasushi Okazaki, Hidehiko Sugino, Christoph Plass, Masami Muramatsu, Hideo Shibata, Kenji Hirose, Veme M. Chapman, Shigeru Tamatsukuri, Nobuya Sasaki, Shinji Hirotsune, Hisato Okuizumi, Hirosumi Imoto, Yoshihide Hayashizaki, Yasuhiro Furuichi
Publikováno v:
Biochemical and Biophysical Research Communications. 194:1406-1412
A new method for target cloning of DNA fragments corresponding to spots on the two-dimensional restriction landmark genomic scanning (RLGS) profile has been developed (targeted spot cloning). We used a Not I restriction trapper to select target DNA f
Autor:
Isao Sakaida, Michio Sata, Koichi Uchida, Yoshiyasu Karino, Joji Toyota, Tatsuya Ide, Takao Tamesa, Yusuke Fujita, Kazuhiko Sakamoto, Shigeru Tamatsukuri, Koichiro Sakata, Toshiaki Miura, Norio Iizuka, Ryoichi Tsunedomi, Kiwamu Okita, Michihisa Iida, Eiji Ando, Satoyoshi Yamashita, Yoshihiko Hamamoto, Yoshihiro Tokuhisa, Shuji Terai, Masahito Tsutsui, Hideo Ishitsuka, Naoki Kimura, Masaaki Oka, Toyoki Moribe
Publikováno v:
Clinica chimica acta; international journal of clinical chemistry. 412(1-2)
Background There are few blood tests for an efficient detection of hepatocellular carcinoma (HCC) associated with hepatitis C virus (HCV) infection. Methods The abilities of quantitative analyses of 7 genes hypermethylation in serum DNA, α-fetoprote
Autor:
Norio Iizuka, Hideo Ishitsuka, Ryouichi Tsunedomi, Yusuke Fujita, Michihisa Iida, Isao Sakaida, Naoki Kimura, Toyoki Moribe, Yoshihiko Hamamoto, Masahito Tsutsui, Toshiaki Miura, Kazuhiko Sakamoto, Masaaki Oka, Yoshihiro Tokuhisa, Takao Tamesa, Shigeru Tamatsukuri
Publikováno v:
Clinica chimica acta; international journal of clinical chemistry. 411(7-8)
Background Prognosis of hepatocellular carcinoma (HCC) remains poor because of high recurrence rate. We examined preoperatively the methylated CCND2 gene levels present in the serum following release from HCC cells as a prognosis predictor in patient
Autor:
Takao Tamesa, Masaaki Oka, Kazuhiko Sakamoto, Yoshihiko Hamamoto, Toshiaki Miura, Shigeru Tamatsukuri, Hideo Ishitsuka, Naoki Kimura, Toyoki Moribe, Norio Iizuka
Publikováno v:
International journal of cancer. 125(2)
The current study was conducted to identify robust methylation markers and their combinations that may prove useful for the diagnosis of early hepatocellular carcinoma (HCC). To achieve this, we performed in silico CpG mapping, direct sequencing and
Autor:
Takao Tamesa, Kazuhiko Sakamoto, Norio Iizuka, Shigeru Tamatsukuri, Toyoki Moribe, Masaaki Oka, Hideo Ishitsuka, Markus Stark, Yoshihiko Hamamoto, Toshiaki Miura
Publikováno v:
International journal of oncology. 33(5)
A genome-wide study using expression profiles of 12,600 genes was conducted to identify methylated genes that could be used for early diagnosis of hepatocellular carcinoma (HCC). Of the 12,600 genes examined, we identified 23 genes with significantly