Výsledky vyhledávání - "Shigeru, Kawano"

Efficient synthesis of (R)-3-hydroxypentanenitrile in high enantiomeric excess by enzymatic reduction of 3-oxopentanenitrile

Autor: Shigeru Kawano, Junzo Hasegawa, Yoshihiko Yasohara

Publikováno v: Applied Microbiology and Biotechnology. 98:5891-5900

(R)-3-Hydroxypentanenitrile (HPN) is an important intermediate in the synthesis of an immunosuppressive inosine 5′-monophosphate dehydrogenase inhibitor. An efficient enzymatic procedure for the synthesis of (R)-HPN with over 99 % enantiomeric exce

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::60af5eda326ae97b065816ae360e5c91
https://doi.org/10.1007/s00253-014-5674-y

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Purification and Characterization of a Novel (S)-Enantioselective Transaminase fromPseudomonas fluorescensKNK08-18 for the Synthesis of Optically Active Amines

Autor: Noriyuki Ito, Junzo Hasegawa, Shigeru Kawano, Yoshihiko Yasohara

Publikováno v: Bioscience, Biotechnology, and Biochemistry. 75:2093-2098

Pseudomonas fluorescens KNK08-18, showing (S)-selective transaminase activity, was isolated from soil by an enrichment culture method using (S)-7-methoxy-2-aminotetraline as the main nitrogen source. A transaminase was purified from the strain to hom

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0cf1f914f5e09ad963fb62055cf5a202
https://doi.org/10.1271/bbb.110240

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Purification and Characterization of an NADH-Dependent Alcohol Dehydrogenase fromCandida marisfor the Synthesis of Optically Active 1-(Pyridyl)ethanol Derivatives

Autor: Shigeru Kawano, Junzo Hasegawa, Miho Yano, Yoshihiko Yasohara

Publikováno v: Bioscience, Biotechnology, and Biochemistry. 75:1055-1060

A novel (R)-specific alcohol dehydrogenase (AFPDH) produced by Candida maris IFO10003 was purified to homogeneity by ammonium sulfate fractionation, DEAE-Toyopearl, and Phenyl-Toyopearl, and characterized. The relative molecular mass of the native en

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b0b291ed1cb74e4a4eebda3407e591ad
https://doi.org/10.1271/bbb.100528

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Efficient Preparation of (R)-3-Hydroxypentanenitrile with High Enantiomeric Excess by Enzymatic Reduction with Subsequent Enhancement of the Optical Purity by Lipase-Catalyzed Ester Hydrolysis

Autor: Yoshihiko Yasohara, Junzo Hasegawa, Shigeru Kawano

Publikováno v: Bioscience, Biotechnology, and Biochemistry. 76:1796-1798

An efficient chemo-enzymatic procedure for the synthesis of (R)-3-hydroxypentanenitrile (1) with over 99% enantiomeric excess using two enzymatic reactions was successfully established. Initial enantioselective enzymatic reduction of 3-oxopentanenitr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9c9295b46b36c889d037975da7c4c2dd
https://doi.org/10.1271/bbb.120331

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[Future outlook on the guidelines for the management of pneumonia--special reference to development of higher quality clinical guidelines (discussion)]

Autor: Yoshinori, Hasegawa, Shigeru, Kawano, Kazunori, Asano, Masahiro, Yoshida

Publikováno v: The Japanese journal of antibiotics. 65(5)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::3d0f22c8385c4fa01dcb2ace3430c361
https://pubmed.ncbi.nlm.nih.gov/23383432

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Nucleotide sequence of the neutral protease gene (nprL) from Lactobacillus sp. and characterization of the enzyme

Autor: Masahiro Takagi, Tadayuki Imanaka, Takuya Maeda, Shigeru Kawano

Publikováno v: Journal of Fermentation and Bioengineering. 77:339-346

The neutral protease gene (nprL) from Lactobacillus sp. no. 1 was cloned and sequenced. Cloning was performed with Bacillus subtilis DB104 [his nprR2 nprE18 ΔaprA3] as a host and pISA ts412 as a vector plasmid. The protease-productive clone harbored

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::6f644eadda8d726e58962aaeb4a20693
https://doi.org/10.1016/0922-338x(94)90001-9

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Nucleotide sequence and possible functional mechanism of the transcriptional activator gene (preL) for neutral protease from Lactobacillus sp

Autor: Tadayuki Imanaka, Takuya Maeda, Masahiro Takagi, Shigeru Kawano

Publikováno v: Journal of Fermentation and Bioengineering. 77:347-351

A positive regulatory gene ( preL ) from Lactobacillus sp. no. 1, which enhanced production of neutral protease (NprL), was cloned in Bacillus subtilis DB104 together with the nprL structural gene. When nprL was expressed with the regulatory gene ( p

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::28052474e97ad63ef8473b4438d5429d
https://doi.org/10.1016/0922-338x(94)90002-7

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Cloning and overexpression of an NADH-dependent alcohol dehydrogenase gene from Candida maris involved in (R)-selective reduction of 5-acetylfuro[2,3-c]pyridine

Autor: Junzo Hasegawa, Miho Yano, Yoshihiko Yasohara, Shigeru Kawano

Publikováno v: Bioscience, biotechnology, and biochemistry. 75(11)

5-((R)-1-Hydroxyethyl)-furo[2,3-c]pyridine ((R)-FPH) is a useful chiral building block in the synthesis of pharmaceuticals. An NADH-dependent alcohol dehydrogenase (AFPDH) isolated from Candida maris catalyzed the reduction of 5-acetylfuro[2,3-c]pyri

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::839d5bebabfb033e9c6faa294f006895
https://pubmed.ncbi.nlm.nih.gov/22056439

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