Feasibility study of on-site solid-state enzyme production by Aspergillus oryzae

Autor: Satoru Shinkawa, Shigenobu Mitsuzawa

Publikováno v: Biotechnology for Biofuels, Vol 13, Iss 1, Pp 1-15 (2020)

Abstract Background The development of biorefinery systems that use lignocellulosic biomass as a renewable carbon source to produce fuels and chemicals is attracting increasing attention. The process cost of enzymatic saccharification of biomass is a

Externí odkaz: https://doaj.org/article/fb83be346fc24e7cbc0f807b6ee9a49f

Fluidic Patch Device to Sample Sweat for Accurate Measurement of Sweat Rate and Chemical Composition

Autor: Tomomi Sato, Narihiko Kondo, Nicola Gerrett, Satoru Shinkawa, Tomoaki Ohashi, Shigenobu Mitsuzawa, Ryo Miyake

Publikováno v: Analytical chemistry, 92(23), 15534-15541. American Chemical Society
Ohashi, T, Gerrett, N, Shinkawa, S, Sato, T, Miyake, R, Kondo, N & Mitsuzawa, S 2020, ' Fluidic Patch Device to Sample Sweat for Accurate Measurement of Sweat Rate and Chemical Composition : A Proof-of-Concept Study ', Analytical chemistry, vol. 92, no. 23, pp. 15534-15541 . https://doi.org/10.1021/acs.analchem.0c03466

Sweat sensors that can continuously sample sweat are critical for determining the time-dependent physiological responses occurring in normal daily life. Here, a new device, termed fluidic patch, for collecting human sweat samples at defined time inte

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e2a646874d983997925bab4b0a1bec67
https://hdl.handle.net/1871.1/f253d05d-f1f2-4907-9bc5-89df30bc56ca

Direct Liquid-Culture Screening for Evaluating the Production of Heterologous Proteins Using an Auxotrophic Mutant of Aspergillus Oryzae

Autor: Shigenobu Mitsuzawa, Satoru Shinkawa

Publikováno v: Journal of Visualized Experiments.

Aspergillus oryzae, a filamentous fungus, is one of the most widely used hosts for industrial applications including large-scale production of proteins. A polyethylene glycol (PEG)-mediated protoplast transformation method is generally used for the i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::6f9c0f20a67d7bb8744e3bc1c572900f
https://doi.org/10.3791/61219

Alanine substitution in cellobiohydrolase provides new insights into substrate threading

Autor: Maiko Fukuura, Satoru Shinkawa, Tadaomi Furuta, Shigenobu Mitsuzawa, Keiichi Kimura

Publikováno v: Scientific Reports
Scientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)

The glycoside hydrolase family 7 (GH7) member cellobiohydrolase (CBH) is a key enzyme that degrades crystalline cellulose, an important structural component of plant cell walls. As GH7 CBH is a major component in the enzyme mixture used to degrade bi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2635695716083aabb44b72badac75171
https://doi.org/10.1038/s41598-017-16434-x

Flow-type apparatus for studying thermotolerance of hyperthermophiles under conditions simulating hydrothermal vent circulation

Autor: Shigenobu Mitsuzawa, Ken Takai, Shigeru Deguchi, Kaoru Tsujii, Koki Horikoshi

Publikováno v: Deep Sea Research Part I: Oceanographic Research Papers. 52:1085-1092

A flow-type apparatus has been developed to study thermotolerance of microorganisms under conditions simulating submarine hydrothermal circulation. The apparatus is designed so that microorganisms can be exposed to high temperature for periods of onl

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::bdb6082194aff086c49355a180b31020
https://doi.org/10.1016/j.dsr.2004.11.020