Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Shigeko Yamazaki"'
Autor:
Jeffrey C. Moore, Michael G. Sturr, Shigeko Yamazaki, Beth Junker, J. Leporati, K. McLoughlin, Randolph Greasham, Michel Chartrain
Publikováno v:
Bioprocess and Biosystems Engineering. 24:39-49
Optimization of pilot-scale enzyme production is described for the case of an extracellular protease and an intracellular esterase. Media optimization was conducted to reduce medium costs and to determine the effect of various defined ingredients as
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c137a560b1e12e23af6a6c643bcebc5f
https://doi.org/10.1007/s004490100230
https://doi.org/10.1007/s004490100230
Autor:
Woo-Baeg Choi, Michel Chartrain, Shigeko Yamazaki, Hywyn R.O. Churchill, Randolph Greasham, Ralph P. Volante, Shashikant Patel, Joseph J. Lynch
Publikováno v:
Journal of Molecular Catalysis B: Enzymatic. 8:285-288
The screening of 310 microbial strains yielded eight as suitable biocatalysts for the asymmetric bioreduction of a highly hindered bisaryl ketone to its corresponding alcohols. The production of both enantiomers with elevated optical purity (ee>96%)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6ea00f28311aa28771bbc260bda815a0
https://doi.org/10.1016/s1381-1177(99)00103-4
https://doi.org/10.1016/s1381-1177(99)00103-4
Autor:
James M. McNamara, Dalian Zhao, G. Hunt, Tao Wang, Barry C. Buckland, Shigeko Yamazaki, Edward J. J. Grabowski, Christopher Roberge, P. Salmon, Michel Chartrain, Randolph Greasham, John Y. L. Chung, David J. Roush, R. Olewinski
Publikováno v:
Enzyme and Microbial Technology. 25:489-496
A microbial screen identified the yeast Candida sorbophila MY 1833 as a suitable biocatalyst for the asymmetric bioreduction of a ketone (2-(4-nitro-phenyl)-N-(2-oxo-2-pyridin-3-yl-ethyl)-acetamide) to its corresponding (R) alcohol [(R)-N-(2-hydroxy-
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ecb5f6b25fc2fe0b8bc67d6dfaf06663
https://doi.org/10.1016/s0141-0229(99)00093-9
https://doi.org/10.1016/s0141-0229(99)00093-9
Publikováno v:
Journal of Chromatography A. 627:137-143
A rapid reversed-phase HPLC assay useful for fermentation and downstream process development was developed for monitoring transforming growth factor-α- Pseudomonas aeruginosa exotoxin A 40 (TGFα-PE40). This protein is a chimeric recombinant protein
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9202d96c896fa9aa5559db465ac71cd5
https://doi.org/10.1016/0021-9673(92)87193-c
https://doi.org/10.1016/0021-9673(92)87193-c
Autor:
Gautam Sanyal, Robert A. Copeland, Robert W. Williams, Robert D. Sitrin, Anne J. Halfpenny, Mark W. Bruner, C. Russell Middaugh, Hanlee Ji, Shigeko Yamazaki, Dorothy Marquis-Omer, Karen C. Thompson, Wayne K. Herber, James A. Ryan, Kenneth A. Thomas
Publikováno v:
Archives of biochemistry and biophysics. 289(1)
The secondary and tertiary structure of recombinant human acidic fibroblast growth factor (aFGF) has been characterized by a variety of spectroscopic methods. Native aFGF consists of ca. 55% beta-sheet, 20% turn, 10% alpha-helix, and 15% disordered p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fdbbbe2848e578abc617a7e1f1194169
https://pubmed.ncbi.nlm.nih.gov/1716876
https://pubmed.ncbi.nlm.nih.gov/1716876
Publikováno v:
Tetrahedron Letters. 26:351-354
To elucidate the stereospecificity of the selenium-containing hydrogenase from Methanocuccus vannielii , ( R ) - and ( S ) - [4- 2 H 1 -3,4-dihydro-7-hydroxy-1-hydroxyethylquinolinone were synthesized as authentic samples for comparison with the comp
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c14e8535726df62481b84fab68d00b79
https://doi.org/10.1016/s0040-4039(01)80815-7
https://doi.org/10.1016/s0040-4039(01)80815-7
Autor:
Shoji Hara, Shigeko Yamazaki
Publikováno v:
BUNSEKI KAGAKU. 17:1544-1547
Improvements were carried out on Bhalla's method of dry-column chromatography which has been used for preparation and extension of thin-layer chromatography (tlc). Moreover, a pressure type ascending apparatus was made, in which standard dyes and som
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::26bde38a8c884b8a427b65aa62bf2510
https://doi.org/10.2116/bunsekikagaku.17.1544
https://doi.org/10.2116/bunsekikagaku.17.1544
Publikováno v:
Proceedings of the National Academy of Sciences. 82:1364-1366
Reduction of 7,8-didemethyl-8-hydroxy-[5-2H]-5-deazariboflavin by the selenium-containing hydrogenase from Methanococcus vannielii gave a C-5 chirally labeled 1,5-dihydro derivative. The absolute configuration of the chiral label was shown to be (R)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::13058cf180b92eff1105724700f18f14
https://doi.org/10.1073/pnas.82.5.1364
https://doi.org/10.1073/pnas.82.5.1364
Autor:
Emilio A. Emini, Arpi Hagopian, Nancy Dunn, Ronald W. Ellis, William J. Miller, Shigeko Yamazaki, Peter J. Kniskern, Pamela D Burke
Publikováno v:
Hepatology (Baltimore, Md.). 8(1)
The entire surface protein of hepatitis B virus sero-type ayw containing the preS (preS1+preS2) and S domains has been expressed in the yeast Saccharomyces cerevisiae. Yeast containing a recombinant plasmid utilizing a constitutive promoter did not e
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8562b4111f82fe2ca092ccf7eb48eb42
https://pubmed.ncbi.nlm.nih.gov/2448217
https://pubmed.ncbi.nlm.nih.gov/2448217
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America. 81(22)
High-level expression of the p21 protein product of the BALB murine sarcoma virus v-ras gene (similar to the product of the Harvey murine sarcoma virus v-Ha-ras gene) has been reported recently, and highly purified preparations of this protein have b
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d68667796c7bddc50a74c410296c0c90
https://pubmed.ncbi.nlm.nih.gov/6438627
https://pubmed.ncbi.nlm.nih.gov/6438627