Zobrazeno 1 - 10
of 30
pro vyhledávání: '"Shigehito, Ikushima"'
Autor:
Minetaka Sugiyama, Shigehito Ikushima, Toshimasa Nakazawa, Yoshinobu Kaneko, Satoshi Harashima
Publikováno v:
BioTechniques, Vol 38, Iss 6, Pp 909-914 (2005)
Chromosome engineering is playing an increasingly important role in the functional analysis of genomes. A simple and efficient technology for manipulating large chromosomal segments is key to advancing these analyses. Here we describe a simple but in
Externí odkaz:
https://doaj.org/article/8a2259a996fc4ad8b6411eb0f9d25b3b
Publikováno v:
PLoS ONE, Vol 7, Iss 5, p e37226 (2012)
The industrially important food-yeast Candida utilis is a Crabtree effect-negative yeast used to produce valuable chemicals and recombinant proteins. In the present study, we conducted whole genome sequencing and phylogenetic analysis of C. utilis, w
Externí odkaz:
https://doaj.org/article/221c15a70f584f68ba15b6ba5bdf4e29
Autor:
Shigehito Ikushima, Mika Miyake, Takayoshi Kirisako, Yukihiro Yada, Yoshihiko Sugihara, Daisuke Fujiwara
Publikováno v:
Clinical, Cosmetic and Investigational Dermatology. 11:115-124
Purpose The present study examined the effect of ingestion of Koji extract containing 14-dehydroergosterol (14-DHE), prepared from Aspergillus kawachii NBRC4308, on improvement of skin conditions among healthy volunteers. Subjects and methods In a ra
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5003ceee2e86b50c8bccf07f1dbf36d6
https://doi.org/10.2147/ccid.s152979
https://doi.org/10.2147/ccid.s152979
Autor:
Jef D. Boeke, Shigehito Ikushima
Publikováno v:
ACS Synthetic Biology. 6:497-506
Here we describe the development of tightly regulated expression switches in yeast, by engineering distant homologues of Escherichia coli TetR, including the transcriptional regulator PhlF from Pseudomonas and others. Previous studies demonstrated th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5c0933c9fc23a3cb202cbecea31f12b3
https://doi.org/10.1021/acssynbio.6b00205
https://doi.org/10.1021/acssynbio.6b00205
Publikováno v:
G3: Genes | Genomes | Genetics. 10/1/2015, Vol. 5 Issue 10, p1983-1990. 8p.
Autor:
Yoshihiko, Sugihara, Shigehito, Ikushima, Mika, Miyake, Takayoshi, Kirisako, Yukihiro, Yada, Daisuke, Fujiwara
Publikováno v:
Clinical, Cosmetic and Investigational Dermatology
Purpose The present study examined the effect of ingestion of Koji extract containing 14-dehydroergosterol (14-DHE), prepared from Aspergillus kawachii NBRC4308, on improvement of skin conditions among healthy volunteers. Subjects and methods In a ra
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::4b6d9762f6ba8ae9dd97698eb27a6e6c
https://pubmed.ncbi.nlm.nih.gov/29563825
https://pubmed.ncbi.nlm.nih.gov/29563825
Publikováno v:
G3: Genes|Genomes|Genetics
Here we describe the engineering of a distant homolog of the Tet repressor, CamR, isolated from Pseudomonas putida, that is regulated by camphor, a very inexpensive small molecule (at micromolar concentrations) for use in Saccharomyces cerevisiae. Th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::894d2194ea32218cd6a39511cf136744
https://doi.org/10.1534/g3.114.012765
https://doi.org/10.1534/g3.114.012765
Autor:
Katrina Caravelli, Neta Agmon, J. Andrew Martin, Woo Jin Choi, Leslie A. Mitchell, Yizhi Cai, Giovanni Stracquadanio, James Chuang, Jef D. Boeke, Allen Zheng, Shigehito Ikushima
Publikováno v:
ACS Synthetic Biology. 4:853-859
We have adapted the Golden Gate DNA assembly method to the assembly of transcription units (TUs) for the yeast Saccharomyces cerevisiae, in a method we call yeast Golden Gate (yGG). yGG allows for the easy assembly of TUs consisting of promoters (PRO
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a0934f114411067004eb4df4eac84c31
https://doi.org/10.1021/sb500372z
https://doi.org/10.1021/sb500372z
Autor:
Neta, Agmon, Zuojian, Tang, Kun, Yang, Ben, Sutter, Shigehito, Ikushima, Yizhi, Cai, Katrina, Caravelli, James A, Martin, Xiaoji, Sun, Woo Jin, Choi, Allen, Zhang, Giovanni, Stracquadanio, Haiping, Hao, Benjamin P, Tu, David, Fenyo, Joel S, Bader, Jef D, Boeke
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America. 114(8)
As the use of synthetic biology grows, there is an increasing need to ensure biocontainment both to protect engineered proprietary strains and to mitigate potential inadvertent or advertent release to the environment. Here, we screen for the best-per
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::b27cf09aa6041fa000a35bdcaec7e6d7
https://pubmed.ncbi.nlm.nih.gov/28174266
https://pubmed.ncbi.nlm.nih.gov/28174266
Autor:
Haiping Hao, Benjamin P. Tu, Zuojian Tang, Shigehito Ikushima, Kun Yang, James A. Martin, Joel S. Bader, David Fenyö, Woo Jin Choi, Giovanni Stracquadanio, Xiaoji Sun, Neta Agmon, Yizhi Cai, Allen Zhang, Katrina Caravelli, Jef D. Boeke, Ben Sutter
Publikováno v:
Agmon, N, Tang, Z, Yang, K, Sutter, B, Ikushima, S, Cai, Y, Caravelli, K, Martin, J A, Sun, X, Choi, W J, Zhang, A, Stracquadanio, G, Hao, H, Tu, B P, Fenyo, D, Bader, J S & Boeke, J D 2017, ' Low escape-rate genome safeguards with minimal molecular perturbation of Saccharomyces cerevisiae ', Proceedings of the National Academy of Sciences, vol. 114, no. 8, pp. E1470-E1479 . https://doi.org/10.1073/pnas.1621250114
As the use of synthetic biology both in industry and in academia grows, there is an increasing need to ensure biocontainment. There is growing interest in engineering bacterial- and yeast-based safeguard (SG) strains. First-generation SGs were based
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e933f37e20391a63b9ac9a2854c46de5
https://hdl.handle.net/20.500.11820/4a2578d0-7cf2-4019-bcf3-45abd950c18f
https://hdl.handle.net/20.500.11820/4a2578d0-7cf2-4019-bcf3-45abd950c18f
