Zobrazeno 1 - 10
of 68
pro vyhledávání: '"Shigehiro Nakajima"'
Publikováno v:
Journal of Neuroscience Methods. 167:258-267
We have developed dissociated primary cultures of the dorsal raphe nucleus from postnatal 9–12-day-old rats. The nucleus was dissected out from brain slices, dissociated, and cultured over a glial feeder layer. Serotonin immunocytochemistry reveale
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1a362a599835e3aa5ada2cedcdf65c6e
https://doi.org/10.1016/j.jneumeth.2007.08.018
https://doi.org/10.1016/j.jneumeth.2007.08.018
Autor:
Shigehiro Nakajima, Takeharu Kawano, Christina V. Floreani, Tohru Kozasa, Peng Zhao, Yasuko Nakajima
Publikováno v:
Molecular Pharmacology. 71:1179-1184
Activation of substance P receptors, which are coupled to Galpha(q), inhibits the Kir3.1/3.2 channels, resulting in neuronal excitation. We have shown previously that this channel inactivation is not caused by reduction of the phosphatidylinositol 4,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f11851f59d84c775095324e7e5711675
https://doi.org/10.1124/mol.106.032508
https://doi.org/10.1124/mol.106.032508
Autor:
Tohru Kozasa, Yasuko Nakajima, Peng Zhao, Qi Zhao, Shigehiro Nakajima, Maki Koike-Tani, John M. Collins, Takeharu Kawano
Publikováno v:
The Journal of Physiology. 564:489-500
Certain transmitters inhibit Kir3 (GIRK) channels, resulting in neuronal excitation. We analysed signalling mechanisms for substance P (SP)-induced Kir3 inhibition in relation to the role of phosphatidylinositol 4,5-bisphosphate (PIP2). SP rapidly
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::eb97b269b6e9575aca8df456e659d6da
https://doi.org/10.1113/jphysiol.2004.079285
https://doi.org/10.1113/jphysiol.2004.079285
Publikováno v:
Neuroscience Letters. 358:63-67
G protein-coupled inward rectifier potassium channels (GIRK, Kir3) play a crucial role in determining neuronal excitability. Currently, four mammalian GIRK members (GIRK1-4) have been genetically identified. We have been investigating physiological p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::58514aaf9dfd5d9104472994cea76001
https://doi.org/10.1016/j.neulet.2003.12.104
https://doi.org/10.1016/j.neulet.2003.12.104
Publikováno v:
Journal of Neuroscience Methods. 132:177-184
The tuberomammillary nucleus (TMN) in the hypothalamus is the sole source of histamine in the brain. This nucleus, by innervating various brain regions, plays an important role for vital functions such as arousal and appetite. We have developed disso
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9320d586327a88e73f325a9cf8360de6
https://doi.org/10.1016/j.jneumeth.2003.09.008
https://doi.org/10.1016/j.jneumeth.2003.09.008
Publikováno v:
Journal of Neurophysiology. 90:693-702
Orexins (hypocretins) are recently discovered excitatory transmitters implicated in arousal and sleep. Yet, their ionic and signal transduction mechanisms have not been fully clarified. Here we show that orexins suppress G-protein–coupled inward re
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a2e9b94d1e8b8c2fd0256922e04c0910
https://doi.org/10.1152/jn.00001.2003
https://doi.org/10.1152/jn.00001.2003
Autor:
Hiroko Nakata, Pamela M. Sternweis, Yasuko Nakajima, Shigehiro Nakajima, Abla Albsoul-Younes, Tohru Kozasa, Peng Zhao
Publikováno v:
Journal of Biological Chemistry. 276:12712-12717
G protein-coupled inward rectifier K(+) channels (GIRK channels) are activated directly by the G protein betagamma subunit. The crystal structure of the G protein betagamma subunits reveals that the beta subunit consists of an N-terminal alpha helix
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b91932ca52987578dae7a90035c2f4a2
https://doi.org/10.1074/jbc.m011231200
https://doi.org/10.1074/jbc.m011231200
Autor:
Hiroshi Itoh, Yoshito Kaziro, Abla Albsoul-Younes, Shigehiro Nakajima, Yasuko Nakajima, Takeharu Kawano, Shinju Y. Watanabe
Publikováno v:
Neuroscience Research. 33:71-78
We investigated the efficiency of transfecting primary cultured rat postnatal brain neurons (substantia nigra pars compacta neurons and locus coeruleus neurons) with cDNA encoding GFP (jellyfish green fluorescent protein) using a calcium phosphate me
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bf38aa3022a21b001beff908013dfaa3
https://doi.org/10.1016/s0168-0102(98)00113-8
https://doi.org/10.1016/s0168-0102(98)00113-8
Publikováno v:
The Journal of Physiology. 502:559-567
1. Types of G proteins (G protein alpha-subunit subtypes) which mediate the activation of inward rectifier K+ currents by somatostatin (somatotrophin release-inhibiting factor, SRIF) were determined in cultured locus coeruleus neurones from newborn r
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::971b6b0aeca5f0624fb3fcb9e55ebf36
https://doi.org/10.1111/j.1469-7793.1997.559bj.x
https://doi.org/10.1111/j.1469-7793.1997.559bj.x
Autor:
Yasuko Nakajima, J. Yasufuku-Takano, Tohru Kozasa, William D. Singer, Shigehiro Nakajima, Koji Takano
Publikováno v:
Journal of Neurophysiology. 76:2131-2136
1. Substance P (SP) induces a slow neuronal excitation in cholinergic neurons from the nucleus basalis by suppressing an inwardly rectifying K+ current (Kir). We have determined which G protein alpha-subunit mediates this SP effect. 2. After intracel
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::79fbd123e4c21cafdea78bdef1d9314d
https://doi.org/10.1152/jn.1996.76.3.2131
https://doi.org/10.1152/jn.1996.76.3.2131