Selective Cytotoxicity of Dihydroorotate Dehydrogenase Inhibitors to Human Cancer Cells Under Hypoxia and Nutrient-Deprived Conditions

Autor: Yukiko Miyazaki, Daniel K. Inaoka, Tomoo Shiba, Hiroyuki Saimoto, Takaya Sakura, Eri Amalia, Yasutoshi Kido, Chika Sakai, Mari Nakamura, Anthony L. Moore, Shigeharu Harada, Kiyoshi Kita

Publikováno v: Frontiers in Pharmacology, Vol 9 (2018)

Human dihydroorotate dehydrogenase (HsDHODH) is a key enzyme of pyrimidine de novo biosynthesis pathway. It is located on the mitochondrial inner membrane and contributes to the respiratory chain by shuttling electrons to the ubiquinone pool. We have

Externí odkaz: https://doaj.org/article/4343e700cc7f4f4caa097f95f6941062

Structural basis for substrate specificity of l ‐methionine decarboxylase

Autor: Masaki Murota, Kenji Inagaki, Shigeharu Harada, Yuki Onoue, Dan Sato, Tomoo Shiba, Atsushi Okawa, Masaya Hayashi, Junko Inagaki, Takashi Tamura

Publikováno v: Protein Sci

l ‐Methionine decarboxylase (MetDC) from Streptomyces sp. 590 is a vitamin B(6)‐dependent enzyme and catalyzes the non‐oxidative decarboxylation of l ‐methionine to produce 3‐methylthiopropylamine and carbon dioxide. We present here the cry

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::77c7c1f111d718574f247e8e63d87dbd
https://doi.org/10.1002/pro.4027

Pharmacophore modeling for anti-Chagas drug design using the fragment molecular orbital method.

Autor: Ryunosuke Yoshino, Nobuaki Yasuo, Daniel Ken Inaoka, Yohsuke Hagiwara, Kazuki Ohno, Masaya Orita, Masayuki Inoue, Tomoo Shiba, Shigeharu Harada, Teruki Honma, Emmanuel Oluwadare Balogun, Josmar Rodrigues da Rocha, Carlos Alberto Montanari, Kiyoshi Kita, Masakazu Sekijima

Publikováno v: PLoS ONE, Vol 10, Iss 5, p e0125829 (2015)

Chagas disease, caused by the parasite Trypanosoma cruzi, is a neglected tropical disease that causes severe human health problems. To develop a new chemotherapeutic agent for the treatment of Chagas disease, we predicted a pharmacophore model for T.

Externí odkaz: https://doaj.org/article/4b0bac0f018a4bb7a714642595f2c480

Insights into the ubiquinol/dioxygen binding and proton relay pathways of the alternative oxidase

Autor: Shigeharu Harada, Masayuki Inoue, Chiaki Tsuge, Satoshi Ueda, Anthony L. Moore, Kiyoshi Kita, Gen Takahashi, Tomoo Shiba, Yasutoshi Kido, Daniel Ken Inaoka, Takeshi Nara, Emmanuel Oluwadare Balogun, Luke Young, Hiroyuki Saimoto, Teruki Honma, Akiko Tanaka

Publikováno v: Biochimica et Biophysica Acta (BBA) - Bioenergetics. 1860:375-382

The alternative oxidase (AOX) is a monotopic diiron carboxylate protein which catalyzes the four-electron reduction of dioxygen to water by ubiquinol. Although we have recently determined the crystal structure of Trypanosoma brucei AOX (TAO) in the p

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d7c20d746bc75a374355dd829611c494
https://doi.org/10.1016/j.bbabio.2019.03.008

The ASCT/SCS cycle fuels mitochondrial ATP and acetate production in Trypanosoma brucei

Autor: Daniel Ken Inaoka, Keisuke Fukuda, Emmanuel Oluwadare Balogun, Michael Boshart, Muriel Mazet, Yoann Millerioux, Kenji Hirayama, Kota Mochizuki, Frédéric Bringaud, Tomoo Shiba, Shigeharu Harada, Hana Kurasawa, Kiyoshi Kita

Publikováno v: Biochimica biophysica acta (BBA)-Bioenergetics
Biochimica biophysica acta (BBA)-Bioenergetics, Elsevier, 2020, 1861 (11), pp.148283. ⟨10.1016/j.bbabio.2020.148283⟩
Biochimica et Biophysica Acta. Bioenergetics

Acetate:succinate CoA transferase (ASCT) is a mitochondrial enzyme that catalyzes the production of acetate and succinyl-CoA, which is coupled to ATP production with succinyl-CoA synthetase (SCS) in a process called the ASCT/SCS cycle. This cycle has

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e66d59bd4a132d226291e1fe78b57801
https://hal.archives-ouvertes.fr/hal-03009591/file/TbASCT-Manuscript-woTC-200609-2-pdf.pdf