Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Shi-Gan Ling"'
Autor:
Shi-Gan Ling, Li Tang, Jing He, Rui Huang, Bingshui Xiu, Heqiu Zhang, Xiaoyan Feng, Kun Chen, Xiqin Yang, Feng He
Publikováno v:
Molecular Biotechnology. 54:58-67
In order to overcome the instability of CpG ODN in vivo, sequence diversity, and individual differences, eleven CpG ODN fragments were meticulously selected and linked to form a Multi-CpG, which were repeatedly inserted into the cloning vector pUC19
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b41120f39217fe9571c1e2d5064556d4
https://doi.org/10.1007/s12033-012-9544-5
https://doi.org/10.1007/s12033-012-9544-5
Autor:
Kun Chen, Jing He, Shi-Gan Ling, Guohua Wang, Xiqin Yang, Xiaoyan Feng, Heqiu Zhang, Cuixia Zhu, Guanzhong Bai, Xiao-Guo Song, Bingshui Xiu
Publikováno v:
Protein & Peptide Letters. 18:839-847
Based on B cell epitope predictions, a recombinant antigen with multiple epitopes from four Hepatitis C Virus fragments (C, NS3, NS4 and NS5) were engineered. The recombinant gene was then highly expressed in E. coli. The non-modified and C-terminal-
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8bf1e9ccaf2ac850a9ab4b6077924a40
https://doi.org/10.2174/092986611795714014
https://doi.org/10.2174/092986611795714014
Autor:
Shi-Gan Ling, Xiao-Guo Song, Cuixia Zhu, Kun Chen, Bingshui Xiu, Xiaoyan Feng, Guohua Wang, Heqiu Zhang, Jing He
Publikováno v:
Journal of virological methods. 171(1)
A double-antigen sandwich ELISA was developed a detection of HCV antibodies by a recombinant multi-epitope HCV antigen and a biotin-streptavidin amplification system. Three plasma specimens from 1708 individuals who were suspected previously to be HC
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::230604b32c761d6958af1cb1faf0d556
https://pubmed.ncbi.nlm.nih.gov/21029749
https://pubmed.ncbi.nlm.nih.gov/21029749
Publikováno v:
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. 22(1)
To prepare recombinant IL-1 fusion protein as the substrate for the HCV NS3 serine protease.NS5A-B gene fragment (2,412-2,427aa) synthesized by PCR was subcloned into prokaryotic expression vector pBVIL1 to fuse with IL-1 gene and the recombinant vec
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::38bdfa0edb15ad8fbb4bf4041cfd03bb
https://pubmed.ncbi.nlm.nih.gov/16388743
https://pubmed.ncbi.nlm.nih.gov/16388743
Autor:
Ya-Ping Gao, Wei Liu, Shi-Gan Ling, Chuan Liu, Zhong-Tao Xin, Jie Zhang, Yan-ning Xue, Ningsheng Shao, Jie Dong, Bo Dong
Publikováno v:
Journal of immunological methods. 293(1-2)
A novel subtractive fluorescence-activated cell-sorting (FACS) strategy using a model system is described here to identify disease-specific (DS) epitopes from a bacterially displayed random peptide library. In this process, preimmune serum was used a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::065e8aeeae4649549405fa9a21f01b69
https://pubmed.ncbi.nlm.nih.gov/15541273
https://pubmed.ncbi.nlm.nih.gov/15541273
Autor:
He-qiu, Zhang, Guo-hua, Wang, Kun, Chen, Bing-shui, Xiu, Xiao-guo, Song, He-zhong, Liu, Shi-gan, Ling
Publikováno v:
Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology. 11(12)
To detect humoral immune response against different function regions of hepatitis C virus (HCV) in chronic patients, and further to investigate the correlativity between anti-HCV antibody titers and HCV RNA concentration.Using recombinant dominate ep
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::252b836eb769cc01fa0cd3b1ffbe8ddd
https://pubmed.ncbi.nlm.nih.gov/14697143
https://pubmed.ncbi.nlm.nih.gov/14697143
Autor:
Ning Sheng Shao, Zhong Tao Xin, Jie Zhang, Ya Ping Gao, Yan Ning Xue, Chuan Liu, Can Quan Mao, An Zhao, Shi Gan Ling
Publikováno v:
Vaccine. 21(27-30)
Bacterially-displayed peptide libraries have been widely used as an alternative to phage-displayed peptide libraries in screening epitopes or mimotopes of antibodies. Using a protective monoclonal antibody (mAb) 3B9 against hepatitis B virus (HBV) pr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::89fab4e9ef0fdd1f69b79047e958303a
https://pubmed.ncbi.nlm.nih.gov/14505920
https://pubmed.ncbi.nlm.nih.gov/14505920
Autor:
Wen-xie Xu, Maochang Liu, Shi-gan Ling, Yu He, Gui-qiang Wang, Henghui Zhang, Xiao-guo Song, Wen-hua Piao, Heqiu Zhang, Zai-liu Li
Publikováno v:
Protein expression and purification. 35(2)
Tetramer analysis is a novel technique in immunological research that has dramatically changed our knowledge of the immune response to pathogens, tumors and autoimmune disease. Through the formation of major histocompatibility complex (MHC)-peptide t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1e67ec69d1d07abd8f7d83aa20f7c469
https://pubmed.ncbi.nlm.nih.gov/15135395
https://pubmed.ncbi.nlm.nih.gov/15135395
AIM: To analyze the amino acid sequences of hypervariable region 1 (HVR1) of HCV isolates in China and to construct a combinatorial chimeric HVR1 protein having a very broad high cross-reactivity. METHODS: All of the published HVR1 sequences from Chi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d0a732213542ef2be3932243ebb0c6ad
https://europepmc.org/articles/PMC4611795/
https://europepmc.org/articles/PMC4611795/
Autor:
Heqiu Zhang, Guohua Wang, Kun Chen, Shi-Gan Ling, Xiao-Guo Song, Baoguang Han, Li Meng, Fang Xia, Xiankai Ma
Hepatitis B virus (HBV) is a DNA virus with an envelope, its membrane protein gene preS/S have three parts-preS1, preS2 and S, there is a translation initiation codon ATG in each of them. By different ATG, the membrane protein gene can be translated
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d87c9e7a6abfae317dc695df3a62859e
https://europepmc.org/articles/PMC4688807/
https://europepmc.org/articles/PMC4688807/