Zobrazeno 1 - 10
of 31
pro vyhledávání: '"Shepherd, Jack W."'
Autor:
Shepherd, Jack W, Guilbaud, Sebastien, Zhou, Zhaokun, Howard, Jamieson, Burman, Matthew, Schaefer, Charley, Kerrigan, Adam, Steele-King, Clare, Noy, Agnes, Leake, Mark C
Biopolymer topology is critical for determining interactions inside cell environments, exemplified by DNA where its response to mechanical perturbation is as important as biochemical properties to its cellular roles. The dynamic structures of chiral
Externí odkaz:
http://arxiv.org/abs/2401.10087
Publikováno v:
J R Soc Interface . 2022 Aug;19(193):20220437
The RecA protein and RecBCD complex are key bacterial components for the maintenance and repair of DNA, RecBCD a helicase-nuclease that uses homologous recombination to resolve double-stranded DNA breaks and also facilitating decoration of single-str
Externí odkaz:
http://arxiv.org/abs/2110.01573
Autor:
Shepherd, Jack W, Leake, Mark C
Far from being a passive information store, the genome is a mechanically dynamic and diverse system in which torsion and tension fluctuate and combine to determine structure and help regulate gene expression. Much of this mechanical perturbation is d
Externí odkaz:
http://arxiv.org/abs/2108.13752
Autor:
Lecinski, Sarah, Shepherd, Jack W, Frame, Lewis, Hayton, Imogen, MacDonald, Chris, Leake, Mark C
Cell division, aging, and stress recovery triggers spatial reorganization of cellular components in the cytoplasm, including membrane bound organelles, with molecular changes in their compositions and structures. However, it is not clear how these ev
Externí odkaz:
http://arxiv.org/abs/2107.14073
As camera pixel arrays have grown larger and faster, and optical microscopy techniques ever more refined, there has been an explosion in the quantity of data acquired during routine light microcopy. At the single-molecule level, analysis involves mul
Externí odkaz:
http://arxiv.org/abs/2103.10164
Publikováno v:
2021 J. Phys. Photonics 3 034010
Super-resolution microscopy has catalyzed valuable insights into the sub-cellular, mechanistic details of many different biological processes across a wide range of cell types. Fluorescence polarization spectroscopy tools have also enabled important
Externí odkaz:
http://arxiv.org/abs/2101.00048
Autor:
Shepherd, Jack W, Leake, Mark C
Here, we report analysis and summary of research in the field of localization microscopy for optical imaging. We introduce the basic elements of super-resolved localization microscopy methods for PALM and STORM, commonly used both in vivo and in vitr
Externí odkaz:
http://arxiv.org/abs/2011.03296
Autor:
Shepherd, Jack W, Lecinski, Sarah, Wragg, Jasmine, Shashkova, Sviatlana, MacDonald, Chris, Leake, Mark C
The physical and chemical environment inside cells is of fundamental importance to all life but has traditionally been difficult to determine on a subcellular basis. Here we combine cutting-edge genomically integrated FRET biosensing to readout local
Externí odkaz:
http://arxiv.org/abs/2010.10862
The double-helical structure of DNA results from canonical base pairing and stacking interactions. However, variations from steady-state conformations result from mechanical perturbations in cells. These different topologies have physiological releva
Externí odkaz:
http://arxiv.org/abs/1904.02576
Autor:
Shepherd, Jack W, Lecinski, Sarah, Wragg, Jasmine, Shashkova, Sviatlana, MacDonald, Chris, Leake, Mark C
Publikováno v:
In Methods September 2021 193:54-61
