Direct observation of dynamic protein interactions involving human microtubules using solid-state NMR spectroscopy

Autor: Yanzhang Luo, ShengQi Xiang, Peter Jan Hooikaas, Laura van Bezouwen, A. S. Jijumon, Carsten Janke, Friedrich Förster, Anna Akhmanova, Marc Baldus

Publikováno v: Nature Communications, Vol 11, Iss 1, Pp 1-10 (2020)

Microtubule (MT) organization is regulated by many microtubule-associated proteins (MAPs) that contain intrinsically disordered regions. Here authors produce [13C, 15N] labeled, functional microtubules from human cells for solid-state NMR which allow

Externí odkaz: https://doaj.org/article/6857f9ffb0814bc9a9cc46cdf3ff87e4

Structural plasticity in human heterochromatin protein 1β.

Autor: Francesca Munari, Nasrollah Rezaei-Ghaleh, Shengqi Xiang, Wolfgang Fischle, Markus Zweckstetter

Publikováno v: PLoS ONE, Vol 8, Iss 4, p e60887 (2013)

As essential components of the molecular machine assembling heterochromatin in eukaryotes, HP1 (Heterochromatin Protein 1) proteins are key regulators of genome function. While several high-resolution structures of the two globular regions of HP1, ch

Externí odkaz: https://doaj.org/article/94f925db5f664815a27736b73ce193fd

Structural analysis of the intrinsically disordered splicing factor Spp2 and its binding to the DEAH-box ATPase Prp2

Autor: Andreas Schmitt, ShengQi Xiang, Henning Urlaub, Romina V. Hofele, Markus Zweckstetter, Filippo Favretto, Piotr Neumann, Florian Hamann, Ralf Ficner

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America
Proceedings of the National Academy of Sciences of the United States of America 117(6), 2948-2956 (2020). doi:10.1073/pnas.1907960117

Proceedings of the National Academy of Sciences of the United States of America 117(6), 2948 - 2956 (2020). doi:10.1073/pnas.1907960117
The spliceosome consists of five small RNAs and more than 100 proteins. Almost 50% of the human spliceosomal

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::25489a886498558306d0df62dea4e879
http://europepmc.org/articles/PMC7022188

Solid-State NMR Spectroscopy for Studying Microtubules and Microtubule-Associated Proteins

Autor: Yanzhang, Luo, Shengqi, Xiang, Alessandra Lucini, Paioni, Agnes, Adler, Peter Jan, Hooikaas, A S, Jijumon, Carsten, Janke, Anna, Akhmanova, Marc, Baldus

Publikováno v: Methods in molecular biology (Clifton, N.J.). 2305

In this chapter, we describe the preparatory and spectroscopic procedures for conducting solid-state NMR experiments on microtubules (MTs) obtained from human cells and their complexes with microtubule-associated proteins (MAPs). Next to labeling and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::10ff13fede983b64d555298f21a2e5eb
https://pubmed.ncbi.nlm.nih.gov/33950391

Characterization of nucleosome sediments for protein interaction studies by solid-state NMR spectroscopy

Autor: Ulric B. le Paige, ShengQi Xiang, Marco M. R. M. Hendrix, Yi Zhang, Markus Weingarth, Alexandre M. J. J. Bonvin, Tatiana G. Kutateladze, Ilja K. Voets, Marc Baldus, Hugo van Ingen

Regulation of DNA-templated processes such as gene transcription and DNA repair depend on the interaction of a wide range of proteins to the nucleosome, the fundamental building block of chromatin. Both solution and solid-state NMR spectroscopy have

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::31b011d9c7230ae26479e9dc3b3fde24
https://doi.org/10.5194/mr-2021-21