Diversity oriented biosynthesis via accelerated evolution of modular gene clusters.

Autor: Wlodek A; Isomerase Therapeutics Ltd., Chesterford Research Park, Cambridge, CB10 1XL, UK., Kendrew SG; Biotica Technology Ltd., Chesterford Research Park, Cambridge, CB10 1XL, UK.; Engineered Biodesign Limited, Cambridge, CB22 3GN, UK., Coates NJ; Isomerase Therapeutics Ltd., Chesterford Research Park, Cambridge, CB10 1XL, UK.; Biotica Technology Ltd., Chesterford Research Park, Cambridge, CB10 1XL, UK., Hold A; Isomerase Therapeutics Ltd., Chesterford Research Park, Cambridge, CB10 1XL, UK., Pogwizd J; Isomerase Therapeutics Ltd., Chesterford Research Park, Cambridge, CB10 1XL, UK., Rudder S; Isomerase Therapeutics Ltd., Chesterford Research Park, Cambridge, CB10 1XL, UK., Sheehan LS; Isomerase Therapeutics Ltd., Chesterford Research Park, Cambridge, CB10 1XL, UK.; Biotica Technology Ltd., Chesterford Research Park, Cambridge, CB10 1XL, UK., Higginbotham SJ; Isomerase Therapeutics Ltd., Chesterford Research Park, Cambridge, CB10 1XL, UK., Stanley-Smith AE; Isomerase Therapeutics Ltd., Chesterford Research Park, Cambridge, CB10 1XL, UK.; Biotica Technology Ltd., Chesterford Research Park, Cambridge, CB10 1XL, UK., Warneck T; Biotica Technology Ltd., Chesterford Research Park, Cambridge, CB10 1XL, UK., Nur-E-Alam M; Biotica Technology Ltd., Chesterford Research Park, Cambridge, CB10 1XL, UK.; Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, 12372, Saudi Arabia., Radzom M; Biotica Technology Ltd., Chesterford Research Park, Cambridge, CB10 1XL, UK.; BASF SE, Speyerer Str. 2, Limburgerhof, 67117, Germany., Martin CJ; Biotica Technology Ltd., Chesterford Research Park, Cambridge, CB10 1XL, UK., Overvoorde L; Isomerase Therapeutics Ltd., Chesterford Research Park, Cambridge, CB10 1XL, UK., Samborskyy M; Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QW, UK., Alt S; Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK., Heine D; Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK., Carter GT; Chemical and Screening Sciences, Wyeth Pharmaceuticals, 401 North Middletown Road, Pearl River, NY, 10965, USA., Graziani EI; Chemical and Screening Sciences, Wyeth Pharmaceuticals, 401 North Middletown Road, Pearl River, NY, 10965, USA.; Medicine Discovery Network-Synthetic Biology, Pfizer Worldwide R&D, 445 Eastern Point Rd., Groton, CT, 06340, USA., Koehn FE; Chemical and Screening Sciences, Wyeth Pharmaceuticals, 401 North Middletown Road, Pearl River, NY, 10965, USA., McDonald L; Chemical and Screening Sciences, Wyeth Pharmaceuticals, 401 North Middletown Road, Pearl River, NY, 10965, USA., Alanine A; Roche Innovation Center Basel, Pharmaceutical Research and Early Development (PRED), Basel, CH-4070, Switzerland., Rodríguez Sarmiento RM; Roche Innovation Center Basel, Pharmaceutical Research and Early Development (PRED), Basel, CH-4070, Switzerland., Chao SK; Roche Innovation Center Basel, Pharmaceutical Research and Early Development (PRED), Basel, CH-4070, Switzerland., Ratni H; Roche Innovation Center Basel, Pharmaceutical Research and Early Development (PRED), Basel, CH-4070, Switzerland., Steward L; Roche Innovation Center Basel, Pharmaceutical Research and Early Development (PRED), Basel, CH-4070, Switzerland., Norville IH; Defence Science and Technology Laboratory, Porton Down, PO17 6AD, UK., Sarkar-Tyson M; Defence Science and Technology Laboratory, Porton Down, PO17 6AD, UK.; Marshall Centre for Infectious Diseases, School of Biomedical Sciences, University of Western Australia, Monash Avenue, Nedlands, WA, 6009, Australia., Moss SJ; Isomerase Therapeutics Ltd., Chesterford Research Park, Cambridge, CB10 1XL, UK.; Biotica Technology Ltd., Chesterford Research Park, Cambridge, CB10 1XL, UK., Leadlay PF; Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QW, UK., Wilkinson B; Isomerase Therapeutics Ltd., Chesterford Research Park, Cambridge, CB10 1XL, UK. barrie.wilkinson@jic.ac.uk.; Biotica Technology Ltd., Chesterford Research Park, Cambridge, CB10 1XL, UK. barrie.wilkinson@jic.ac.uk.; Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK. barrie.wilkinson@jic.ac.uk., Gregory MA; Isomerase Therapeutics Ltd., Chesterford Research Park, Cambridge, CB10 1XL, UK. matt.gregory@isomerase.co.uk.; Biotica Technology Ltd., Chesterford Research Park, Cambridge, CB10 1XL, UK. matt.gregory@isomerase.co.uk.

Publikováno v: Nature communications [Nat Commun] 2017 Oct 31; Vol. 8 (1), pp. 1206. Date of Electronic Publication: 2017 Oct 31.

Engineered biosynthesis of hybrid macrolide polyketides containing D-angolosamine and D-mycaminose moieties.

Autor: Schell U; Biotica Technology Ltd, Chesterford Research Park, Little Chesterford, Saffron Walden, Essex, CB10 1XL, UK., Haydock SF, Kaja AL, Carletti I, Lill RE, Read E, Sheehan LS, Low L, Fernandez MJ, Grolle F, McArthur HA, Sheridan RM, Leadlay PF, Wilkinson B, Gaisser S

Publikováno v: Organic & biomolecular chemistry [Org Biomol Chem] 2008 Sep 21; Vol. 6 (18), pp. 3315-27. Date of Electronic Publication: 2008 Jul 28.

Rapamycin biosynthesis: Elucidation of gene product function.

Autor: Gregory MA; Biotica, Chesterford Research Park, Little Chesterford, UK CB10 1XL., Hong H, Lill RE, Gaisser S, Petkovic H, Low L, Sheehan LS, Carletti I, Ready SJ, Ward MJ, Kaja AL, Weston AJ, Challis IR, Leadlay PF, Martin CJ, Wilkinson B, Sheridan RM

Publikováno v: Organic & biomolecular chemistry [Org Biomol Chem] 2006 Oct 07; Vol. 4 (19), pp. 3565-8. Date of Electronic Publication: 2006 Aug 22.