Single-nucleus and single-cell transcriptomes compared in matched cortical cell types.

Autor: Trygve E Bakken, Rebecca D Hodge, Jeremy A Miller, Zizhen Yao, Thuc Nghi Nguyen, Brian Aevermann, Eliza Barkan, Darren Bertagnolli, Tamara Casper, Nick Dee, Emma Garren, Jeff Goldy, Lucas T Graybuck, Matthew Kroll, Roger S Lasken, Kanan Lathia, Sheana Parry, Christine Rimorin, Richard H Scheuermann, Nicholas J Schork, Soraya I Shehata, Michael Tieu, John W Phillips, Amy Bernard, Kimberly A Smith, Hongkui Zeng, Ed S Lein, Bosiljka Tasic

Publikováno v: PLoS ONE, Vol 13, Iss 12, p e0209648 (2018)

Transcriptomic profiling of complex tissues by single-nucleus RNA-sequencing (snRNA-seq) affords some advantages over single-cell RNA-sequencing (scRNA-seq). snRNA-seq provides less biased cellular coverage, does not appear to suffer cell isolation-b

Externí odkaz: https://doaj.org/article/a582d6cca35342eaac24aa2cf90cf4a8

Classification of electrophysiological and morphological neuron types in the mouse visual cortex

Autor: David Sandman, Brian Lee, Michael Hawrylycz, Sara Kebede, Tom Egdorf, David Reid, Rob Young, Nivretta Thatra, Stefan Mihalas, David Feng, John W. Phillips, Rebecca de Frates, DiJon Hill, Cliff Slaughterbeck, Samuel R Josephsen, Tamara Casper, Xiaoxiao Liu, Hanchuan Peng, Peter Chong, Colin Farrell, Zhi Zhou, Sheana Parry, Jed Perkins, Brian Long, Susan M. Sunkin, Matthew Kroll, Krissy Brouner, Melissa Gorham, Aaron Szafer, Wayne Wakeman, Hong Gu, Marissa Garwood, Daniel Park, Kristen Hadley, Michael S. Fisher, Lydia Potekhina, Ed Lein, Alice Mukora, Hongkui Zeng, Nick Dee, Aaron Oldre, Lindsay Ng, Thomas Braun, Grace Williams, Tracy Lemon, Julie A. Harris, Medea McGraw, Nadezhda Dotson, Philip R. Nicovich, Amanda Gary, Rusty Mann, Alex M. Henry, Caroline Habel, Samuel Dingman, Katherine E. Link, Nathalie Gaudreault, Gilberto J. Soler-Llavina, Thuc Nghi Nguyen, Nicole Blesie, Bosiljka Tasic, Lydia Ng, Christine Cuhaciyan, Tim Jarsky, Keith B. Godfrey, Costas A. Anastassiou, Kirsten Crichton, Josef Sulc, Martin Schroedter, Dan Castelli, Miranda Robertson, Amy Bernard, Lisa Kim, Songlin Ding, Alyse Doperalski, Nathan W. Gouwens, Herman Tung, Tsega Desta, Corinne Teeter, James Harrington, Jonathan T. Ting, Kris Bickley, Anton Arkhipov, Kiet Ngo, Changkyu Lee, Jim Berg, Agata Budzillo, Emma Garren, Tanya L. Daigle, Christof Koch, Rachel A. Dalley, Eliza Barkan, Staci A. Sorensen, Gabe J. Murphy, Shiella Caldejon, Naz Taskin

Publikováno v: Nat Neurosci

Understanding the diversity of cell types in the brain has been an enduring challenge and requires detailed characterization of individual neurons in multiple dimensions. To systematically profile morpho-electric properties of mammalian neurons, we e

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1e51c6a86c36dcef44deb5b36ad8fc78
https://doi.org/10.1038/s41593-019-0417-0

Conserved cell types with divergent features in human versus mouse cortex

Autor: Elliot R. Thomsen, Ahmed Mahfouz, Saroja Somasundaram, Aaron Oldre, Bosiljka Tasic, Songlin Ding, Richard H. Scheuermann, Daniel Hirschstein, Thomas Höllt, Christine Rimorin, Thuc Nghi Nguyen, Jennie L. Close, John W. Phillips, Lydia Ng, Jeff Goldy, Darren Bertagnolli, Amy Bernard, Zizhen Yao, Boaz P. Levi, Trygve E. Bakken, Soraya I. Shehata, Susan M. Sunkin, Osnat Penn, Michael Tieu, Allison Beller, Boudewijn P. F. Lelieveldt, Jeffrey G. Ojemann, Shannon Reynolds, Michael Hawrylycz, Jeroen Eggermont, Medea McGraw, Ryder P. Gwinn, Sheana Parry, Kimberly A. Smith, Brian Long, Olivia Fong, Zoe Maltzer, Rafael Yuste, David Feng, Julie Nyhus, Rebecca D. Hodge, Ed Lein, Jeremy A. Miller, Brian D. Aevermann, Gerald Quon, Emma Garren, Christof Koch, Aaron Szafer, Nick Dee, Nadiya V. Shapovalova, Rachel A. Dalley, Tamara Casper, Mohamed Keshk, Nelson Johansen, Krissy Brouner, Andrew L. Ko, Allan R. Jones, Eliza Barkan, Hongkui Zeng, Richard G. Ellenbogen, C. Dirk Keene, Kanan Lathia, Lucas T. Graybuck, Charles Cobbs

Publikováno v: Nature, vol 573, iss 7772
Nature, 573(7772), 61

Elucidating the cellular architecture of the human cerebral cortex is central to understanding our cognitive abilities and susceptibility to disease. Here we used single-nucleus RNA-sequencing analysis to perform a comprehensive study of cell types i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::45cb2e1e03c5b89538fe61c3d31c48fc
https://doi.org/10.1038/s41586-019-1506-7

Adult Mouse Cortical Cell Taxonomy by Single Cell Transcriptomics

Autor: Linda Madisen, Kimberly A. Smith, Darren Bertagnolli, Amy Bernard, Tim Jarsky, Jeff Goldy, Tim A. Dolbeare, Hongkui Zeng, Lucas T. Gray, Christof Koch, Tae Kyung Kim, Susan M. Sunkin, Boaz P. Levi, Changkyu Lee, Bosiljka Tasic, Nadiya V. Shapovalova, Zizhen Yao, Michael Hawrylycz, Staci A. Sorensen, Thuc Nghi Nguyen, Sheana Parry, Vilas Menon

Publikováno v: Nature neuroscience

Nervous systems are composed of various cell types, but the extent of cell type diversity is poorly understood. We constructed a cellular taxonomy of one cortical region, primary visual cortex, in adult mice on the basis of single-cell RNA sequencing

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc6a2809a0ab068684746c992cbd6541
http://europepmc.org/articles/PMC4985242

Classification of electrophysiological and morphological types in mouse visual cortex

Autor: Tom Egdorf, Rebecca de Frates, Emma Garren, Sara Kebede, Peter Chong, John W. Phillips, Nivretta Thatra, Samuel R Josephsen, Philip R. Nicovich, Tim Jarsky, Xiaoxiao Liu, Susan M. Sunkin, Brian Lee, Keith B. Godfrey, Matthew Kroll, Nicole Blesie, Bosiljka Tasic, Amy Bernard, Lisa Kim, Costas A. Anastassiou, Kristen Hadley, Staci A. Sorensen, Thuc Nghi Nguyen, Martin Schroedter, Corinne Teeter, Kirsten Crichton, Josef Sulc, Rachel A. Dalley, David Feng, Tracy Lemon, Michael Hawrylycz, Miranda Robertson, Christine Cuhaciyan, Eliza Barkan, Shiella Caldejon, Tsega Desta, Kris Bickley, Dan Castelli, Wayne Wakeman, Herman Tung, Hongkui Zeng, Grace Williams, Nadezhda Dotson, Rusty Mann, Tamara Casper, Anton Arkhipov, Daniel Park, Sheana Parry, Jed Perkins, Alyse Doperalski, Brian Long, Thomas Braun, Christof Koch, Gabe J. Murphy, Aaron Oldre, Changkyu Lee, Colin Farrell, Medea McGraw, Amanda Gary, Kiet Ngo, Melissa Gorham, Naz Taskin, Jim Berg, Samuel Dingman, Tanya L. Daigle, Agata Budzillo, Marissa Garwood, Gilberto J. Soler-Llavina, Aaron Szafer, Nick Dee, Jonathan T. Ting, Lydia Ng, Alex M. Henry, James Harrington, Julie A. Harris, Michael S. Fisher, Lindsay Ng, Caroline Habel, Nathalie Gaudreault, Krissy Brouner, David Reid, Lydia Potekhina, Rob Young, DiJon Hill, Cliff Slaughterbeck, Ed Lein, Alice Mukora, David Sandman, Stefan Mihalas, Nathan W. Gouwens, Zhi Zhou, Hanchuan Peng, Hong Gu

Understanding the diversity of cell types in the brain has been an enduring challenge and requires detailed characterization of individual neurons in multiple dimensions. To profile morpho-electric properties of mammalian neurons systematically, we e

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cfca7e9215372424dd682d3a161f1bc4