Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Shawn Siefring"'
Publikováno v:
Journal of Microbiological Methods. 152:135-142
An obstacle to establishing widely useful data acceptance criteria for U.S. Environmental Protection Agency (EPA) qPCR methods has been the unavailability of standardized reference materials. Earlier versions of EPA Methods 1609 and 1611 for enteroco
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e3c47a4474a10cf5bd248963f1bf5f33
https://doi.org/10.1016/j.mimet.2018.07.005
https://doi.org/10.1016/j.mimet.2018.07.005
Autor:
Richard R. Rediske, Mano Sivaganesan, Tiong Gim Aw, Susan Rosenberg, Tami Sivy, Barbara Weberman, Shawn Siefring, Erin A. Dreelin, Julie L. Kinzelman, Kevin Oshima, Abhilasha Shrestha, Richard A. Haugland, Greg Kleinheinz, Samuel Dorevitch, Natasha M. Isaacs, Brian Scull, Asli Aslan, Ben Southwell, Shannon Briggs, Rachel T. Noble
Publikováno v:
Water Res
There is interest in the application of rapid quantitative polymerase chain reaction (qPCR) methods for recreational freshwater quality monitoring of the fecal indicator bacteria Escherichia coli (E. coli). In this study we determined the performance
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d78b93880189ad62bded88d933e0bb02
https://europepmc.org/articles/PMC9994418/
https://europepmc.org/articles/PMC9994418/
Autor:
Asli Aslan, Ben Southwell, Shawn Siefring, Erin A. Dreelin, Tami Sivy, Richard A. Haugland, Julie L. Kinzelman, Abhilasha Shrestha, Natasha M. Isaacs, Rachel T. Noble, Brian Scull, Tiong Gim Aw, Richard R. Rediske, Shannon Briggs, Barbara Weberman, Samuel Dorevitch, Greg Kleinheinz, Kevin Oshima, Susan Rosenberg, Mano Sivaganesan
Publikováno v:
Water Res
There is growing interest in the application of rapid quantitative polymerase chain reaction (qPCR) and other PCR-based methods for recreational water quality monitoring and management programs. This interest has strengthened given the publication of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad16a9990b4ad27fa0c7f73a7ed26415
Autor:
Brian Schnitker, Manju Varma, Shawn Siefring, Richard A. Haugland, Alfred P. Dufour, Sharon P. Nappier, Mano Sivaganesan, Kevin Oshima, Shannon Briggs
Publikováno v:
J Microbiol Methods
Fecal pollution remains a challenge for water quality managers at Great Lakes and inland recreational beaches. The fecal indicator of choice at these beaches is typically Escherichia coli (E. coli), determined by culture-based methods that require ov
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::61c5dadbea98f84c7faa524b88c7b703
https://doi.org/10.1016/j.mimet.2021.106186
https://doi.org/10.1016/j.mimet.2021.106186
Autor:
Kevin Oshima, Stephen B. Weisberg, Mano Sivaganesan, Julie L. Kinzelman, A. Denene Blackwood, Tamara Anan’eva, Katrina V. Gordon, Erin A. Stelzer, V.J. Harwood, John F. Griffith, Yiping Cao, Shawn Siefring, Richard A. Haugland, Christopher D. Sinigalliano, Meredith R. Raith, Rachel T. Noble, Rebecca N. Bushon, Manju Varma
Publikováno v:
Journal of Microbiological Methods. 123:114-125
Quantitative polymerase chain reaction (qPCR) has become a frequently used technique for quantifying enterococci in recreational surface waters, but there are several methodological options. Here we evaluated how three method permutations, type of ma
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::72cee162a3be2f7949cb8cd465f99ad3
https://doi.org/10.1016/j.mimet.2016.01.017
https://doi.org/10.1016/j.mimet.2016.01.017
Publikováno v:
Journal of Microbiological Methods. 87:343-349
DNA extracts from enumerated target organism cells (calibrator samples) have been used for estimating Enterococcus cell equivalent densities in surface waters by a comparative cycle threshold (Ct) qPCR analysis method. To compare surface water Entero
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7993be97c9567b8154587c705a34a28c
https://doi.org/10.1016/j.mimet.2011.09.013
https://doi.org/10.1016/j.mimet.2011.09.013
Publikováno v:
Letters in Applied Microbiology. 52:298-306
Aims: Compare specificity and sensitivity of quantitative PCR (qPCR) assays targeting single and multi-copy gene regions of Escherichia coli. Methods and Results: A previously reported assay targeting the uidA gene (uidA405) was used as the basis for
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::85fef1437af17663e99180bee237994f
https://doi.org/10.1111/j.1472-765x.2010.03001.x
https://doi.org/10.1111/j.1472-765x.2010.03001.x
Publikováno v:
Water Research. 39:559-568
Cell densities of the fecal pollution indicator genus, Enterococcus, were determined by a rapid (3 h or less) quantitative polymerase chain reaction (QPCR) analysis method in 100 ml water samples collected from recreational beaches on Lake Michigan a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1cb9883816651e5cf42f5cd6a059d68d
https://doi.org/10.1016/j.watres.2004.11.011
https://doi.org/10.1016/j.watres.2004.11.011
Publikováno v:
Biotechnology Letters. 25:261-265
A 16S rDNA real-time PCR method was developed to detect Enterococcus faecalis in water samples. The dynamic range for cell detection spanned five logs and the detection limit was determined to be 6 cfu/reaction. The assay was capable of detecting E.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::25b92ad97c97e2e106d42c1d2daf7e9e
https://doi.org/10.1023/a:1022303118122
https://doi.org/10.1023/a:1022303118122
Publikováno v:
Journal of microbiological methods. 112
A method, incorporating recently improved reverse transcriptase-PCR primer/probe assays and including controls for detecting interferences in RNA recovery and analysis, was developed for the direct, culture-independent detection of genetic markers fr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c6e011c4d7257685b875bbb82e5bec79
https://pubmed.ncbi.nlm.nih.gov/25744574
https://pubmed.ncbi.nlm.nih.gov/25744574