Zobrazeno 1 - 10 of 12 pro vyhledávání: '"Shawn E. Ramer"'
1
Catalytic domains of the LAR and CD45 protein tyrosine phosphatases from Escherichia coli expression systems: purification and characterization for specificity and mechanism
Autor: Paul Burn, Shawn E. Ramer, Willi Bannwarth, Haruo Saito, Hyeongjin Cho, Michiyasu Itoh, Eric A. Kitas, Christopher T. Walsh
Publikováno v: Biochemistry. 31:133-138
The cytoplasmic domains of two human transmembrane protein tyrosine phosphatases (PTPases), LAR and CD45, have been expressed in Escherichia coli, purified to near-homogeneity, and compared for catalytic efficiency toward several phosphotyrosine-cont
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d7578e81c98ff704f9c1f9d6d0eb8f87
https://doi.org/10.1021/bi00116a019
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2
Purification and initial characterization of the lymphoid-cell protein-tyrosine kinase p56lck from a baculovirus expression system
Autor: Ana C. Carrera, Christopher T. Walsh, David G. Winkler, Thomas M. Roberts, Shawn E. Ramer
Publikováno v: Proceedings of the National Academy of Sciences of the United States of America. 88(14)
The lymphocyte-specific protein-tyrosine kinase p56lck has been purified 90-fold to approximately 30% purity in 30% yield from a baculovirus expression system by a two-column purification procedure. At least two forms of p56lck were isolated, differi
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6add7ea084878b1e4a8be07bce7c82aa
https://pubmed.ncbi.nlm.nih.gov/2068105
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3
Purification and characterization of a soluble catalytic fragment of the human transmembrane leukocyte antigen related (LAR) protein tyrosine phosphatase from an Escherichia coli expression system
Autor: Willi Bannwarth, Shawn E. Ramer, Haruo Saito, Eric A. Kitas, Hyeongjin Cho, Michiyasu Itoh, Christopher T. Walsh, David G. Winkler, Paul Burn
Publikováno v: Biochemistry. 30(25)
A 350 amino acid soluble fragment of the intracellular catalytic domain of the human transmembrane leukocyte antigen related (LAR) protein tyrosine phosphatase has been purified 17-fold to greater than 90% purity from an Escherichia coli expression v
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bf8d8d4c0a9a07ce955447daf62dbb4b
https://pubmed.ncbi.nlm.nih.gov/1711896
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5
Kinetic characterization of the [3'-32P]coenzyme A/acetyl coenzyme A exchange catalyzed by a three-subunit form of the carbon monoxide dehydrogenase/acetyl-CoA synthase from Clostridium thermoaceticum
Autor: Shawn E. Ramer, Scott A. Raybuck, William H. Orme-Johnson, Christopher T. Walsh
Publikováno v: Biochemistry. 28:4675-4680
The ability of acetyl coenzyme A synthesizing carbon monoxide dehydrogenase isolated from Clostridium thermoaceticum to catalyze the exchange of [3'-32P]coenzyme A with acetyl coenzyme A is studied. This exchange is found to have a rate exceeding tha
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a435466cacbddcfc2301fb7c87761eac
https://doi.org/10.1021/bi00437a025
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6
Investigations of polypeptide biosynthesis: formation of peptide amides
Autor: Hengmiao Cheng, John C. Vederas, Shawn E. Ramer
Publikováno v: Pure and Applied Chemistry. 61:489-492
Peptides having a primary amide functionality at the carboxyl terminus are formed by oxidative cleavage of two carbons from glycine-extended precursors by peptidylglycine a-amidating monooxygenase (PAM) in the presence of oxygen, copper and ascorbic
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::8e05eb1e2fbc5125f893c00eb974e5ae
https://doi.org/10.1351/pac198961030489
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7
Mechanism of formation of serine β-lactones by Mitsunobu cyclization: synthesis and use of L-serine stereospecifically labelled with deuterium at C-3
Autor: John C. Vederas, Richard N. Moore, Shawn E. Ramer
Publikováno v: Canadian Journal of Chemistry. 64:706-713
The ring closure of N-benzyloxycarbonyl-L-serine (1) under Mitsunobu conditions (Ph3P, dimethyl azodicarboxylate, −78 °C) to give the corresponding β-lactone (2) is shown by deuterium and oxygen-18 labelling studies to proceed by hydroxy group ac
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::5e32db1f220d1780111d01dd7e1b9088
https://doi.org/10.1139/v86-113
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10
Biosynthesis of multicolosic acid, a polyketide metabolite from Penicillium multicolor: occurrence of large18O-induced β-isotope shifts in13C n.m.r. spectra
Autor: John C. Vederas, Shawn E. Ramer, John S. E. Holker, Miyuki Kaneda
Publikováno v: J. Chem. Soc., Chem. Commun.. :1099-1100
Fermentation of Penicillium multicolor under an atmosphere containing 18O2 produces multicolosic acid (1) whose methylated derivative (2) displays both α-isotope shifts and unusually large β-isotope shifts in its 13C n.m.r. spectra; the results def
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::b1436b9e873502a75c567cad4e4fd009
https://doi.org/10.1039/c39870001099
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