dSLAM analysis of genome-wide genetic interactions in Saccharomyces cerevisiae

Autor: Daniel S. Yuan, Xiaoling Wang, Siew Loon Ooi, Jef D. Boeke, Pamela B. Meluh, Sharon Sookhai-Mahadeo, Xuewen Pan

Publikováno v: Methods. 41:206-221

Analysis of genetic interactions has been extensively exploited to study gene functions and to dissect pathway structures. One such genetic interaction is synthetic lethality, in which the combination of two non-lethal mutations leads to loss of orga

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c6a6f40f9655fd77a5963140400d24fc
https://doi.org/10.1016/j.ymeth.2006.07.033

Functional profiling of the Saccharomyces cerevisiae genome

Autor: Bart Scherens, Ning Lan, Steeve Veronneau, Anna Astromoff, Matt Curtiss, Ronald W. Davis, Jeffrey N. Strathern, Marleen Voet, Johannes H. Hegemann, Ankuta Lucau-Danila, Petra Ross-Macdonald, Adam M. Deutschbauer, Françoise Foury, Greg Schimmack, Daniel D. Shoemaker, Karen Davis, Rhonda Bangham, Darlene LaBonte, Christopher J. Roberts, Zelek S. Herman, Patrick Flaherty, Stefano Campanaro, Patrice Menard, Michael Snyder, Teresa R. Ward, Adam P. Arkin, Howard Bussey, Linda Riles, Yonghong Yang, Grace Yen, Guri Giaever, K. D. Entian, Kexin Yu, Elaine M. Youngman, Sharon Sookhai-Mahadeo, Jef D. Boeke, Reginald Storms, Daniel F. Jaramillo, Giorgio Valle, José L. Revuelta, Sally Dow, Mark Johnston, Matthias Rose, Mohamed El Bakkoury, Deanna Gotte, Hong Liao, Elizabeth A. Winzeler, Brenda Shafer, Marc Lussier, Chuanyun Luo, Siew Loon Ooi, Peter Philippsen, Angela M. Chu, Guido Volckaert, Rong Mao, Peter Kötter, Keith Anderson, Ching Yun Wang, Julie Wilhelmy, Ulrich Güldener, Li Ni, Lucy Y. Liu, Mark Gerstein, David C. Lamb, Steven L. Kelly, Carla Connelly, Diane E. Kelly, Svenja Hempel, Rocío Benito, David J. Garfinkel, Bruno André, Hong Liang, Sophie Brachat

Publikováno v: Nature. 418:387-391

Determining the effect of gene deletion is a fundamental approach to understanding gene function. Conventional genetic screens exhibit biases, and genes contributing to a phenotype are often missed. We systematically constructed a nearly complete col

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::57bc06ae739927da5c4b01c8dabd24f4
https://doi.org/10.1038/nature00935

Analysis of Genetic Interactions on a Genome-Wide Scale in Budding Yeast: Diploid-Based Synthetic Lethality Analysis by Microarray

Autor: Carol Tiffany, Xiaoling Wang, Brian D. Peyser, Daniel S. Yuan, Pamela B. Meluh, Ou Chen, Rafael A. Irizarry, Xuewen Pan, Sharon Sookhai-Mahadeo, Jef D. Boeke, Forrest Spencer

Publikováno v: Microbial Gene Essentiality: Protocols and Bioinformatics ISBN: 9781588293787

Comprehensive collections of open reading frame (ORF) deletion mutant strains exist for the budding yeast Saccharomyces cerevisiae. With great prescience, these strains were designed with short molecular bar codes or TAGs that uniquely mark each dele

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::bd7b16d44d256374a28f0a7fb7a32978
https://doi.org/10.1007/978-1-59745-321-9_15