Zobrazeno 1 - 10
of 52
pro vyhledávání: '"Sharon S. Krag"'
Autor:
Sharon S. Krag, Jane R. Scocca
Publikováno v:
Gene Amplification in Mammalian Cells
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::bb7f5de0519adaf7dba377332edffec6
https://doi.org/10.1201/9781003066989-21
https://doi.org/10.1201/9781003066989-21
Autor:
Elena F. Jacobson, Raghothama Chaerkady, Robert N. Cole, Robert N. O'Meally, Daniel Wolozny, Deniz Baycin-Hizal, Michael J. Betenbaugh, Harish Nagarajan, Bernhard O. Palsson, Joe Colao, Lily Chen, Vishaldeep Sarkaria, Amit Kumar, Nathan E. Lewis, Hui Zhang, Yuan Tian, David L. Tabb, Sharon S. Krag
Publikováno v:
Journal of Proteome Research. 11:5265-5276
To complement the recent genomic sequencing of Chinese hamster ovary (CHO) cells, proteomic analysis was performed on CHO cells including the cellular proteome, secretome, and glycoproteome using tandem mass spectrometry (MS/MS) of multiple fractions
Publikováno v:
Biochemical and Biophysical Research Communications. 395:36-41
Glycosylation-deficient Chinese Hamster Ovary (CHO) cell lines can be used to expand our understanding of N-glycosylation pathways and to study Congenital Disorders of Glycosylation, diseases caused by defects in the synthesis of N-glycans. The mamma
Publikováno v:
Biochimica et Biophysica Acta (BBA) - General Subjects. 1726:121-137
N-linked glycosylation, a common co-translational modification in eukaryotic cells, involves the transfer of a lipid-linked oligosaccharide onto asparagine residues in a tripeptide sequon on a nascent protein in the lumen of the endoplasmic reticulum
Autor:
Valentina C. Ciccarone, Jullian Jones, David Judd, Christina Giblin, Michael J. Betenbaugh, Tarangsri Nivitchanyong, Sharon S. Krag, Stephen F. Gorfien
Publikováno v:
Biotechnology and Bioengineering. 91:722-732
The inducible T-REx system and other inducible expression systems have been developed in order to control the expression levels of recombinant protein in mammalian cells. In order to study the effects of heterologous protein expression on mammalian h
Publikováno v:
Biochemical and Biophysical Research Communications. 331:379-383
The level of cis-prenyl transferase activity has been implicated in controlling the level of biosynthesis of dolichol and dolichol intermediates. In this study, we isolated a cDNA encoding a human CPT (GenBank Accession No. BE206717 ), which had subs
Autor:
Jessica L. O'Rear, Sharon S. Krag, George J. Quellhorst, Paul T. Englund, Soo Hee Lee, Alvaro Acosta-Serrano, Kuo-Yuan Hwa
Publikováno v:
Eukaryotic Cell. 3:255-263
Concanavalin A (ConA) kills the procyclic (insect) form of Trypanosoma brucei by binding to its major surface glycoprotein, procyclin. We previously isolated a mutant cell line, ConA 1-1, that is less agglutinated and more resistant to ConA killing t
Publikováno v:
Biochemical and Biophysical Research Communications. 312:817-824
Mammalian dolichol-phosphate-mannose (DPM) synthase has three subunits, DPM1, DPM2, and DPM3. In this report, an analysis of the gene and cDNAs of hamster DPM2 is presented. The CHO DPM2 gene has two special features. First, the initiation codon ATG
Autor:
Allan Gottschalk, Michael J. Betenbaugh, Sunny Mai, Sharon S. Krag, Hui Zhang, Daniel Wolozny, Elena F. Jacobson, Deniz Baycin-Hizal
Publikováno v:
Biochemical and biophysical research communications. 453(2)
Voltage-gated ion channels are transmembrane proteins that regulate electrical excitability in cells and are essential components of the electrically active tissues of nerves, muscle and the heart. Potassium channels are one of the largest subfamilie
Autor:
Daniel Wolozny, Deniz Baycin Hizal, Hui Zhang, Elena F. Jacobson, Michael J. Betenbaugh, Yuan Tian, Sharon S. Krag, Joseph Colao
Publikováno v:
Clinical proteomics
Protein glycosylation serves critical roles in the cellular and biological processes of many organisms. Aberrant glycosylation has been associated with many illnesses such as hereditary and chronic diseases like cancer, cardiovascular diseases, neuro