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pro vyhledávání: '"Sharon E. Hutchings"'
Autor:
Gordon Sato, Jennie Mather, Sugayuki Ohasa, Izumi Hayashi, Don B. McClure, Hideo Masui, Sharon E. Hutchings, Jane E. Bottenstein, Richard A. Wolfe, Angie Rizzino, Ginette Serrero, Reen Wu
Publisher Summary This chapter presents the practical details for the growth of cells in serum free hormone supplemented media. In the absence of serum, greater than usual care must be taken in preparation of the synthetic portion of the medium. Care
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b13bac83810c1a6b678c01a86f5da09c
https://doi.org/10.1016/s0076-6879(79)58127-0
https://doi.org/10.1016/s0076-6879(79)58127-0
Autor:
Gordon Sato, Sharon E. Hutchings
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America. 75(2)
HeLa cells grow in a nutritionally complete synthetic medium (Ham's F12) supplemented with insulin, transferrin, hydrocortisone (aldosterone), fibroblast growth factor, and epidermal growth factor. This hormone-supplemented medium supports clonal gro