Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Shan-Jian Zhang"'
Autor:
Marietta Y.W.T. Lee, Sheng Ming Wu, Bao Qing Li, Jinyao Mo, Peng Zhang, Xiao Rong Zeng, Shan Jian Zhang
Publikováno v:
Journal of Biological Chemistry. 273:9561-9569
The catalytic subunit of human DNA polymerase (pol) δ was overexpressed in an active, soluble form by the use of a baculovirus system in insect cells. The recombinant enzyme was separated from endogenous DNA polymerases by phosphocellulose, Mono Q-S
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::824079e78e5503dc587a41d3588916df
https://doi.org/10.1074/jbc.273.16.9561
https://doi.org/10.1074/jbc.273.16.9561
Publikováno v:
Nucleic Acids Research. 25:5041-5046
The identity of DNA replication proteins and cell cycle regulatory proteins which can be found in complexes involving PCNA were investigated by the use of PCNA immobilized on Sepharose 4B. A column containing bovine serum albumin (BSA) bound to Sepha
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::35219b634e5488db09e06bc3dfda0152
https://doi.org/10.1093/nar/25.24.5041
https://doi.org/10.1093/nar/25.24.5041
Autor:
Yubo Sun, Peng Zhang, Marietta Y.W.T. Lee, Yunquan Jiang, Bao Qing Li, N. Lan Toomey, Shan Jian Zhang, Yi Zhou
Publikováno v:
Journal of Biological Chemistry. 272:13013-13018
DNA polymerase delta is a heterodimer consisting of a catalytic subunit of 125 kDa and a small subunit of 50 kDa (p50). We have overexpressed p50 in Escherichia coli and have characterized the recombinant protein. p50 was readily overexpressed using
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3d28bb5bbd89f6636766857908a01410
https://doi.org/10.1074/jbc.272.20.13013
https://doi.org/10.1074/jbc.272.20.13013
Publikováno v:
Biochemistry. 34:10703-10712
Human proliferating cell nuclear antigen (PCNA) was overexpressed in Escherichia coli as a soluble protein. Recombinant PCNA was purified to homogeneity by phosphocellulose, Q-Sepharose, Sephacryl S-200, and hydroxylapatite chromatography. Approximat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::265459a286e756e44617f96069faf7b1
https://doi.org/10.1021/bi00034a002
https://doi.org/10.1021/bi00034a002
Publikováno v:
Archives of Biochemistry and Biophysics. 320:297-304
A monoclonal antibody against human DNA polymerase delta (pol delta) was isolated with properties suitable for its utilization for immunoaffinity chromatography. The antibody was immobilized after periodate oxidation and coupled to a hydrazide-activa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1e1b42ba4d5c84656069c2e70bb520fb
https://doi.org/10.1016/0003-9861(95)90013-6
https://doi.org/10.1016/0003-9861(95)90013-6
Autor:
Xiao-Rong Zeng, Ray-Yuan Chuang, N. Lan Toomey, Shan-Jian Zhang, Marietta Y.W.T. Lee, Peng Zhang, Long-Sheng Chang
Publikováno v:
Journal of Biological Chemistry. 270:7988-7992
Synthetic peptides to selected sequences in human DNA polymerase delta (pol delta) were used to identify the region involved in the interaction of pol delta to proliferating cell nuclear antigen. Peptides corresponding to sequences in five regions in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3912ae7b239ddc7977bc10fc8897fb0e
https://doi.org/10.1074/jbc.270.14.7988
https://doi.org/10.1074/jbc.270.14.7988
Publikováno v:
Journal of Biological Chemistry. 269:13748-13751
We have used antibodies specific for either polymerase delta (pol delta) or its accessory protein, proliferating cell nuclear antigen (PCNA), to demonstrate that they can markedly inhibit the capacity of HeLa nuclear extracts to effect repair of UV-d
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::bbbd2de2d081ff33bd5327334172cc30
https://doi.org/10.1016/s0021-9258(17)36709-1
https://doi.org/10.1016/s0021-9258(17)36709-1
Publikováno v:
Journal of Biological Chemistry. 266:2423-2429
DNA polymerase delta was purified from human placenta and its polymerase catalytic subunit identified as a 125-kDa polypeptide by activity staining. This 125-kDa form of DNA polymerase delta resembles that reported from calf thymus (Lee, M. Y. W. T.,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8b3f419e4f768672eb60a3365801d1ae
https://doi.org/10.1016/s0021-9258(18)52261-4
https://doi.org/10.1016/s0021-9258(18)52261-4
Publikováno v:
Chromosoma. 102
A continuing theme of our laboratory has been the understanding of human DNA polymerases at the structural level. We have purified DNA polymerases delta, epsilon and alpha from human placenta. Monoclonal antibodies to these polymerases were isolated
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6a097aff0aae033b0b1cfa0cb115abe1
https://pubmed.ncbi.nlm.nih.gov/1291233
https://pubmed.ncbi.nlm.nih.gov/1291233
Autor:
Sheng-Ming Wu, Peng Zhang, Xiao Rong Zeng, Shan-Jian Zhang, Jinyao Mo, Bao Qing Li, Marietta Y.W.T. Lee
Publikováno v:
Journal of Biological Chemistry. 273:17978
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b1407c953b6a475be467b9ecda4b350c
https://doi.org/10.1016/s0021-9258(18)80582-8
https://doi.org/10.1016/s0021-9258(18)80582-8