Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Shamik S. Sharma"'
Autor:
Catherine L. Madinger, Michelle L. Cushing, Julie Canovas, Christopher H. Taron, Jack S. Benner, Brian P. Anton, Lauren G. Fields, Shamik S. Sharma
Publikováno v:
PROTEOMICS. 9:4744-4754
A proteomic analysis was performed on spent fermentation medium following bioreactor propagation of a wild-type industrial strain to identify proteins naturally secreted by Kluyveromyces lactis cells. Here, we report changes detected in the K. lactis
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a3d41b29c965c21fd41baa3bacdb8a3d
https://doi.org/10.1002/pmic.200800915
https://doi.org/10.1002/pmic.200800915
Publikováno v:
PROTEOMICS. 8:2714-2723
Secretion of proteins is the most common approach to protein expression in Kluyveromyces lactis. A proteomic analysis was performed on spent fermentation medium following bioreactor propagation of a wild-type industrial strain to identify proteins na
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3097a7ac4995a67f048a3bb9efc64b89
https://doi.org/10.1002/pmic.200700764
https://doi.org/10.1002/pmic.200700764
Publikováno v:
Metabolic Engineering. 9:133-141
Recently, efforts have been made to improve the properties of Escherichia coli as a recombinant host by 'genomic surgery'-deleting large segments of the E. coli K12 MG1655 genome without scars. These excised segments included K-islands, which contain
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d696af0266c0ea076551ea15204fc38a
https://doi.org/10.1016/j.ymben.2006.10.002
https://doi.org/10.1016/j.ymben.2006.10.002
Publikováno v:
Biotechnology and Bioengineering. 98:1056-1070
Highly reduced E. coli strains, MDS40, MDS41, and MDS42, lacking approximately 15% of the genome, were grown to high cell densities to test their ability to produce a recombinant protein with high yields. These strains lack all transposons and insert
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2bfbfa3d2df02ec97fedce7e09744fa2
https://doi.org/10.1002/bit.21491
https://doi.org/10.1002/bit.21491
Publikováno v:
Applied Biochemistry and Biotechnology. 126:093-118
Inteins are self-cleavable proteins that under reducing conditions can be cleaved from a recombinant target protein. Industrially, an intein-based system could potentially reduce production costs of recombinant proteins by facilitating a highly selec
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7e5953ed542115475568e7b0d3f0ba5e
https://doi.org/10.1385/abab:126:2:093
https://doi.org/10.1385/abab:126:2:093
Publikováno v:
Biotechnology Progress. 19:1085-1090
Protein splicing elements (inteins), capable of catalyzing controllable peptide bond cleavage reactions, have been used to separate recombinant proteins from affinity tags during affinity purification. Since the inteins eliminate the use of a proteas
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::12964589eefd44bef36449e8f30205c0
https://doi.org/10.1021/bp034009r
https://doi.org/10.1021/bp034009r
Autor:
David Frisch, Guy Plunkett, Shamik S. Sharma, Buffy Stahl, György Pósfai, Vitaliy Kolisnychenko, Kinga Umenhoffer, Günther M. Keil, Monika de Arruda, Valerie Burland, Tamás Fehér, Frederick R. Blattner, Sarah W. Harcum
Publikováno v:
Science. 312:1044-1046
With the use of synthetic biology, we reduced the Escherichia coli K-12 genome by making planned, precise deletions. The multiple-deletion series (MDS) strains, with genome reductions up to 15%, were designed by identifying nonessential genes and seq
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b3dc51f6d0920d9bf47e81233aabad43
https://doi.org/10.1126/science.1126439
https://doi.org/10.1126/science.1126439
Publikováno v:
Journal of biotechnology. 125(1)
The intein-mediated purification system has the potential to significantly reduce the recovery costs of industrial recombinant proteins. The ability of inteins to catalyze a controllable peptide bond cleavage reaction can be used to separate a recomb
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d817a2f6aac5c176515a5459efaa5d33
https://pubmed.ncbi.nlm.nih.gov/16546284
https://pubmed.ncbi.nlm.nih.gov/16546284