Synthase-selected sorting approach identifies a beta-lactone synthase in a nudibranch symbiotic bacterium

Autor: Mária Džunková, James J. La Clair, Tomáš Tyml, Devin Doud, Frederik Schulz, Samuel Piquer-Esteban, Dafne Porcel Sanchis, Andrew Osborn, David Robinson, Katherine B. Louie, Ben P. Bowen, Robert M. Bowers, Janey Lee, Vicente Arnau, Wladimiro Díaz-Villanueva, Ramunas Stepanauskas, Terrence Gosliner, Shailesh V. Date, Trent R. Northen, Jan-Fang Cheng, Michael D. Burkart, Tanja Woyke

Publikováno v: Microbiome, Vol 11, Iss 1, Pp 1-24 (2023)

Abstract Background Nudibranchs comprise a group of > 6000 marine soft-bodied mollusk species known to use secondary metabolites (natural products) for chemical defense. The full diversity of these metabolites and whether symbiotic microbes are respo

Externí odkaz: https://doaj.org/article/d885a6b36ed6498aa727710bc46feac4

A centimeter-long bacterium with DNA compartmentalized in membrane-bound organelles

Autor: Jean-Marie Volland, Silvina Gonzalez-Rizzo, Olivier Gros, Tomáš Tyml, Natalia Ivanova, Frederik Schulz, Danielle Goudeau, Nathalie H Elisabeth, Nandita Nath, Daniel Udwary, Rex R Malmstrom, Chantal Guidi-Rontani, Susanne Bolte-Kluge, Karen M Davies, Maïtena R Jean, Jean-Louis Mansot, Nigel J Mouncey, Esther Angert, Tanja Woyke, Shailesh V Date

Cells of most bacterial species are around 2 µm in length, with some of the largest specimens reaching 750 µm. Using fluorescence, x-ray, and electron microscopy in conjunction with genome sequencing, we characterized Ca. Thiomargarita magnifica, a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::99a8a25579c091c50a67591bade1789f
https://doi.org/10.1101/2022.02.16.480423

A single-cell genome perspective on studying intracellular associations in unicellular eukaryotes

Autor: Shailesh V. Date, Tomáš Tyml, Tanja Woyke

Publikováno v: Philosophical transactions of the Royal Society of London. Series B, Biological sciences, vol 374, iss 1786
Philosophical Transactions of the Royal Society B: Biological Sciences

Single-cell genomics (SCG) methods provide a unique opportunity to analyse whole genome information at the resolution of an individual cell. While SCG has been extensively used to investigate bacterial and archaeal genomes, the technique has been rar

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::080e6f6f192c055be56c5be52c1b0aee
https://escholarship.org/uc/item/8tb1w40d

De Novo Guanine Biosynthesis but Not the Riboswitch-Regulated Purine Salvage Pathway Is Required for Staphylococcus aureus Infection In Vivo

Autor: Baiwei Lin, Mike Reichelt, Anand Kumar Katakam, Min Xu, Ian R. Monk, Man-Wah Tan, Summer Park, Shailesh V. Date, Cary D. Austin, Donghong Yan, Eric M. Kofoed, Till Maurer, Janice Kim

Publikováno v: Journal of bacteriology. 198(14)

De novo guanine biosynthesis is an evolutionarily conserved pathway that creates sufficient nucleotides to support DNA replication, transcription, and translation. Bacteria can also salvage nutrients from the environment to supplement the de novo pat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cab7ae14b3ac28268ef12c0f4927c9d6
https://pubmed.ncbi.nlm.nih.gov/27161118

A Probabilistic Functional Network of Yeast Genes

Autor: Edward M. Marcotte, Insuk Lee, Shailesh V. Date, Alex T. Adai

Publikováno v: Science. 306:1555-1558

A conceptual framework for integrating diverse functional genomics data was developed by reinterpreting experiments to provide numerical likelihoods that genes are functionally linked. This allows direct comparison and integration of different classe

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::55e8bc733c288c3f8d67619bc1890ed2
https://doi.org/10.1126/science.1099511