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Partial sequencing of the VP2 capsid gene for direct enterovirus genotyping in clinical specimens

Autor: Philippe Berthelot, D. Nasri-Zoghlami, N. Boukhadra, Wafa Ibrahim, Bruno Pozzetto, Shabir Omar, Thomas Bourlet, Sylvie Pillet

Publikováno v: Clinical Microbiology and Infection. 20:O558-O565

Typing of human enterovirus (EV) remains a major goal for diagnostic and epidemiological purposes. Whereas sequencing of the VP1 coding region is the reference standard for EV typing, a method relying on sequencing of the VP2 coding region has been p

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d16d1533375a6179126b1338c9d8507f
https://doi.org/10.1111/1469-0691.12520

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Coxsackievirus B3 replication and persistence in intestinal cells from mice infected orally and in the human CaCo-2 cell line

Autor: Bruno Pozzetto, Olivier Delézay, Rafik Harrath, Mahjoub Aouni, Fatima Douche-Aourik, Thomas Bourlet, Shabir Omar

Publikováno v: Journal of Medical Virology. 74:283-290

Although the transmission of coxsackievirus B3 occurs mainly via the oral route, little is known about the primary replication and persistence of this agent in the intestine. To address this question, BALB/c mice were inoculated by gavage with coxsac

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::76fea17d5cfb727cc5bb4d5dfbc0ddce
https://doi.org/10.1002/jmv.20179

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Detection of enterovirus in human skeletal muscle from patients with chronic inflammatory muscle disease or fibromyalgia and healthy subjects

Autor: Shabir Omar, Rafik Harrath, Léonard Féasson, Thomas Bourlet, Florence Grattard, Fatima Douche-Aourik, Willy Berlier, Christian Denis, Bruno Pozzetto

Publikováno v: Journal of Medical Virology. 71:540-547

Enterovirus RNA has been found previously in specimens of muscle biopsy from patients with idiopathic dilated cardiomyopathy, chronic inflammatory muscle diseases, and fibromyalgia or chronic fatigue syndrome (fibromyalgia/chronic fatigue syndrome).

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::70e99757e3d101e3b8d432f22beecac9
https://doi.org/10.1002/jmv.10531

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Detection of coxsackievirus B3 in intestinal tissue of orally-infected mice by a standardized RT-PCR assay

Autor: Thomas Bourlet, Shabir Omar, Florence Grattard, Bruno Pozzetto

Publikováno v: Clinical and Diagnostic Virology. 8:143-150

Background: Previous studies have reported the role of enteroviruses in chronic diseases, using in-house RT-PCR protocols. A well-standardized PCR assay (Amplicor enterovirus, Produits Roche) designed for the diagnosis of enterovirus meningitis in ce

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a0a2b5efac3d27d5f9a47c3d9b560a9b
https://doi.org/10.1016/s0928-0197(97)00022-6

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Typing of Human Enterovirus by Partial Sequencing of VP2▿

Autor: Mahjoub Aouni, Henia Saoudin, Thomas Bourlet, Bruno Pozzetto, Shabir Omar, Sylvie Pillet, Lamjed Bouslama, Dorsaf Nasri

The sequencing of the VP1 hypervariable region of the human enterovirus (HEV) genome has become the reference test for typing field isolates. This study describes a new strategy for typing HEV at the serotype level that uses a reverse transcription-P

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6f69cd7d66b0c483c06a9efcfe44d40c
https://europepmc.org/articles/PMC1951248/

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Coxsackievirus B3 replication and persistence in intestinal cells from mice infected orally and in the human CaCo-2 cell line

Autor: Rafik, Harrath, Thomas, Bourlet, Olivier, Delézay, Fatima, Douche-Aourik, Shabir, Omar, Mahjoub, Aouni, Bruno, Pozzetto

Publikováno v: Journal of medical virology. 74(2)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::11c4651ad652e250aa69aaf0e6cf0202
https://pubmed.ncbi.nlm.nih.gov/15332278

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Surveillance Network for Herpes Simplex Virus Resistance to Antiviral Drugs: 3-Year Follow-Up

Publikováno v: Journal of Clinical Microbiology
Journal of Clinical Microbiology, American Society for Microbiology, 2004, pp.249

Herpes simplex virus (HSV) infections are very common in the general population and among immunocompromised patients. Acyclovir (ACV) is an effective treatment which is widely used. We deemed it essential to conduct a wide and coordinated survey of t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::13b15c63253b73675aea6dc42da77604
https://europepmc.org/articles/PMC321677/

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Detection of enterovirus in human skeletal muscle from patients with chronic inflammatory muscle disease or fibromyalgia and healthy subjects

Autor: Fatima, Douche-Aourik, Willy, Berlier, Léonard, Féasson, Thomas, Bourlet, Rafik, Harrath, Shabir, Omar, Florence, Grattard, Christian, Denis, Bruno, Pozzetto

Publikováno v: Journal of medical virology. 71(4)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::b314dbc11635c5ef8af23cfb5cfefb58
https://pubmed.ncbi.nlm.nih.gov/14556267

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Comparison of a rapid culture method combining an immunoperoxidase test and a group specific anti-VP1 monoclonal antibody with conventional virus isolation techniques for routine detection of enteroviruses in stools

Autor: Jawhar Gharbi, Mahjoub Aouni, Shabir Omar, Bruno Pozzetto, Thomas Bourlet

Publikováno v: Journal of medical virology. 54(3)

In order to shorten the time required for the detection of enteroviruses in stool specimens, an 18-h immunoperoxidase test combining low-speed centrifugation and the use of a group specific anti-VP1 monoclonal antibody (5-D8/1, Dako) was developed. T

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::289e52b7fa1cacd31ad704aa1856ea92
https://pubmed.ncbi.nlm.nih.gov/9515770

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